Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYAKEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLTLHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIKGYDIITLMEY 4LZA Chain:B ((27-195)) -EIKMMIREIPDFPKKGIKFKDITPVLKDAKAFNYSIEMLAKALEGRKFDLIAAPEARGFLFGAPLAYRLGVGFVPVRKPGKLPAETLSYEYELEYGTDSLEIHKDAVLEGQRVVIVDDLLATGGTIYASAKLVESLGGIVDSIIFLTELTFLDGRKKLDGYDIISLIKF

General information: TITO was launched using: RESULT: Template: 4LZA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 843 -145091 -172.11 -858.53 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -172.11 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_4LZA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LZA-query.scw PDB file : Tito_Scwrl_4LZA.pdb: