Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTIMEIINYLFGGISMKQKIAVLGPGSWGTALAQTLAENGH--------DVRIWGNVPEQ-----IDEINKYHTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPIIIHASKGLEQDTH--KRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQK---------AAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMTRGLAEISRLGVAMGA--NPLTFI-GLSGVGDLIVTCTSVHSRNWRAGNLLG-QGHKLEEVLENM--GMVVEGVATTKAAVELAQQLGV--EMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMENEFSLR 4FGW Chain:B ((33-385)) ----------------KPFKVTVIGSGNWGTTIAKVVAENCKGYPEVFAPIVQMWVFEEEINGEKLTEIINTRHQNVKYLPGITLPDNLVANPDLIDSVKDVDIIVFNIPHQFLPRICSQLKGHVDSHVRAISCLKGFEVGAKGVQLLSSYITEELG----IQCGALSGANIATEVAQEHWSETTVAYHIPKDFRGEGKDVDHKVLKALFHRPYFHVSVIEDVAGISICGALKNVVALGCGFVEGLGWGNNASAAIQRVGLGEIIRFGQMFFPESREETYYQESAGVADLITTCAG--GRNVKVARLMATSGKDAWECEKELLNGQSAQGLITCKEVHEWLETCGSVEDFPLFEAVYQIVYNNYPMKNLPDMIEE------------

General information: TITO was launched using: RESULT: Template: 4FGW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 0 0 NaN 0.00 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : NaN 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.74 QMean score : ?

(partial model without unconserved sides chains): PDB file : Tito_4FGW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FGW-query.scw PDB file : Tito_Scwrl_4FGW.pdb: