Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVETAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVHQDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEVADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAVEVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGETLSLQEFAALSNAMSENMQ 3FUX Chain:C ((4-268)) ------LASPQSVRALLERHGLFADKRFGQNFLVSEAHLRRIVEAARP-FTGPVFEVGPGLGALTRALLEAGAEVTAIEKDLRLRPVLEETLS-GLPVRLVFQDALLYPWEE-------VPQGSLLVANLPYHIATPLVTRLLKT-GRFARLVFLVQKEVAERMTARPKTPAYGVLTLRVAHHAVAERLFDLPPGAFFPPPKVWSSLVRLTPTGAL-----DDPGLFRLVEAAFGKRRKTLLNALAAAGY----PKARVEEALRALGLPPRVRAEELDLEAFRRLREGLE----

General information: TITO was launched using: RESULT: Template: 3FUX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1373 -185917 -135.41 -701.57 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -135.41 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_3FUX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FUX-query.scw PDB file : Tito_Scwrl_3FUX.pdb: