Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQFQGSAKMKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYELDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF 2BL8 Chain:A ((4-103)) ------------NAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLE-------PSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF

General information: TITO was launched using: RESULT: Template: 2BL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 291 -51043 -175.41 -548.85 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -175.41 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.693

(partial model without unconserved sides chains): PDB file : Tito_2BL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BL8-query.scw PDB file : Tito_Scwrl_2BL8.pdb: