Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIGTQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPLHAVDFLADYF--KEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCSPVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLKAITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQG-ISRGQTLVD-RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG 1EZR Chain:A ((3-309)) -RKIILDCDPGIDDAVAIFLAHGNPEIELLAITTVVGNQSLEKVTQNARLVADVAGIVGVPVAAGCTKPLVRGVRNASHIHGETGMGNVSYPPEFKTKLDGRHAVQLIIDLIMSHEPKTITLVPTGGLTNIAMAVRLEPRIVDRVKEVVLMGGGYHT-GNASPVAEFNVFIDPEAAHIVF-NESWNVTMVGLDLTHLALATPAVQKRVREVGTKPAAFMLQILDFYTKVYEKEHDTYGKV-HDPCAVAYVIDPTVMTTERVPVDIELNGALTTGMTVADFRYP---RPKNCR--TQVAVKLDFDKFWCLVIDALER------------

General information: TITO was launched using: RESULT: Template: 1EZR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1842 -105744 -57.41 -348.99 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -57.41 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_1EZR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EZR-query.scw PDB file : Tito_Scwrl_1EZR.pdb: