Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDIVLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVISKENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHLSSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTITEARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE 2FML Chain:A ((3-268)) --QFASKAEEKNYYERQASLAEFLTWYHQQELPEYEKPSLTVDMVLLCYNKEADQLKVLLIQRKGHPFRNSWALPGGFVNRNESTEDSVLRETKEETGVVISQENIEQLHSFSRPDRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVHWFNLERHGQHITLSHEDVEITLDLKTAASLGKDTLAFDHSEIIIKAFNRVVDKMEHEPQVLQVLGKDFTITEARKVFAKFLGVDYRSIDHSNFKKAMTQYFEELGE-------PSKIYQLK-----

General information: TITO was launched using: RESULT: Template: 2FML.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1196 -132644 -110.91 -512.14 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -110.91 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_2FML.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FML-query.scw PDB file : Tito_Scwrl_2FML.pdb: