Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQRDLEKANITIL-PFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFIQNYTSIAVTGSHGKTSTTGLLSHVLS--GVRPTSYLIGDGTG-H----G-DPQAEFFSFEACEYRRHFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVK--KAIFAYGDDAYLRKLKANV--PIYYYGVTE-NDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVIAVAY-FEKLDLKEVAEEMLTFPGVKRRFSEKIV----------ADMTVVDDYAHHPAEIKATIDGARQKYPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQG-NVKIEDLGAKIKKGG----EVI-KENNVSPLL---DYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL 4HV4 Chain:B ((19-484)) -MRRVRHIHFVGIGGAGMGGIAEVLANEGYQISGSDLAP-NSVTQHLTALGAQIYFHHRPENVLDASVVVVSTAISADNPEIVAAREARIPVIRRAEMLAELMRYRHGIAVAGTHGKTTTTAMLSSIYAEAGLDP-TFVNGGLVKAAGTHARLGS-SRYLIAEADESDASFLHLQPMVAIVTNIEAD------DFENLKQTFINFLHNLPFYGRAVMCIDDPVVRELLPRVGRHITTYGFSDDADV-QIASYRQEGPQGHFTLRRQDKPLIEVTLNAPGRHNALNAAAAVAVATEEG-IEDEDILRALVGFQGTGRRFDFLGNFPLAPVNGKEGSAMLVDDYGHHPTEVDATIKAARAGWPDKRIVMLFQPHRYTRTRDLYDDFANVLSQVDVLLMLDVYA-AGEPPIPGADSRALCRTIRNRGKLDPILVPDSESAPEMLAQILNGEDLILVQGAGNIGKIARKLAEHKLQ------

General information: TITO was launched using: RESULT: Template: 4HV4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2379 -59212 -24.89 -139.32 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -24.89 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_4HV4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HV4-query.scw PDB file : Tito_Scwrl_4HV4.pdb: