Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNVAPLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALGGGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPAGVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKD--LLEHAVPIITATLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHAEKIGLSPTGSTAALERILQKFHLPITYD-LSHPDELFHAITHDKKARGSQLNIILLEKIGQAKIV-PVPTETFKDYLGGL 3OKF Chain:A ((33-386)) -ITVNLGERSYPISIGAGLFAN-PALLSLS-AKQKVVIVTNHTVAPLYAPAIISLLDHIGCQHALLELPDGEQYKTLETFNTVMSFLLEHNYSRDVVVIALGGGVIGDLVGFAAACYQRGVDFIQIPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQPKAVVIDTDCLTTLPAREFAAGMAEVIKYGIIYDSAFFDWLEAQMEALYALDEQALTYAIARCCQIKAEVVAQDEK--GIRALLNLGHTFGHAIEAHMGYGNWLHGEAVSAGTVMAAKTAQLQGLIDASQFERILAILKKAHLPVRTPENMTFADFMQHMMRDKKVLAGELRLVLPTSIGTSAVVKGVPEAVIAQAIEY-

General information: TITO was launched using: RESULT: Template: 3OKF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1882 -161868 -86.01 -465.14 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -86.01 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.579

(partial model without unconserved sides chains): PDB file : Tito_3OKF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OKF-query.scw PDB file : Tito_Scwrl_3OKF.pdb: