Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSIAEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAEADCLVDRIQQDET-SIRPLALG-KTREEIKALLAQRLSWYEESATHRINTTNQTPEKIIDTIIERIKSI 1E6C Chain:B ((2-170)) TEPIFMVGARGCGMTTVGRELARALGYEFVDTDIFMQHTSGMTVADVVAAEGWPGFRRRESEALQAVATPNRVVATGGGMVLLEQNRQFMRAHGTVVYLFAPAEELALRLQASLQAHQRPTLTGRPIAEEMEAVLREREALYQDVAHYVVDAT-QPPAAIVCELMQTMRL-

General information: TITO was launched using: RESULT: Template: 1E6C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 706 -53489 -75.76 -320.29 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -75.76 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.673

(partial model without unconserved sides chains): PDB file : Tito_1E6C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E6C-query.scw PDB file : Tito_Scwrl_1E6C.pdb: