Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQEGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAAMRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLDGVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGRPITQSTNPYKAYQTIQTEWS 2YYU Chain:B ((4-235)) ----HTPFIVALDFPSKQEVERFLRPF-AGTPLFVKVGMELYYQEGPAIVAFLKEQGHAVFLDLKLHDIPNTVKQAMKGLARVGADLVNVHAAGGRRMMEAAIEGLDAGTPSGRMRPRCIAVTQLTSTDERMLHEELWISRPLVETVAHYAALAKESGLDGVVCSANEAAFIKERCGASFLAVTPGIRFADDAA----RVVTPRKARALGSDYIVIGRSLTRAADPLRTYARLQHEW-

General information: TITO was launched using: RESULT: Template: 2YYU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1186 -154789 -130.51 -678.90 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -130.51 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.642

(partial model without unconserved sides chains): PDB file : Tito_2YYU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YYU-query.scw PDB file : Tito_Scwrl_2YYU.pdb: