Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSIMVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEE-YNELPIIANVAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKVSSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGLSGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPYICPKLIKELPVRMSELGIESLERLIKEVREERER 1EP3 Chain:A ((5-311)) ---NRLSVKLPGLDLKNPIIPASGCFGFGEEYAKYYDLNKLGSIMVKATTLHPRFGNPTPRVAETASGMLNAIGLQNPGLEVIMTEKLPWLNENFPELPIIANVAGSEEADYVAVCAKIGDAANVKAIELNISCPNVKHGGQAFGTDPEVAAALVKACKAVSKVPLYVKLSPNVTDIVPIAKAVEAAGADGLTMINTLMGVRFDLKTRQPILANITGGLSGPAIKPVALKLIHQVAQDVDIPIIGMGGVANAQDVLEMYMAGASAVAVGTANFADPFVCPKIIDKLPELMDQYRIESLESLIQEVKEGKK-

General information: TITO was launched using: RESULT: Template: 1EP3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1861 -92547 -49.73 -302.44 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -49.73 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_1EP3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EP3-query.scw PDB file : Tito_Scwrl_1EP3.pdb: