Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIEAGLVLFFVFVVLLVLGMPIAISIAF------------SSMMTLLLVIPFDVSAFSSAQKMVGSIN---SFSLIA---IPFFVLSGIIMNN-GGIAKKLVDFAMLFVGRVPGALAHTNVLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTAFIMYSLVAGGASISALFMG-GYLV--GALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQSVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGMFMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGGVKAEAMLKPLLPFY----GVILVVLFLITYFPQIVMWLPNMLS- 5ULM Chain:A ((7-390)) --FRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQQVA-------SDMYCLVGRIYKDMFLDS-NFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHSSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLAND------HMRVIQASEKLFKLKTP--------------------AWYLKSIVETILIYKHFVKPVAKQELVDFWMDFLVE--------ATKT--------------------DVTVVRFPVLIL---------------EPTKIYQPSYLSINNEVE-----EKTISIWHVLPDD-KKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITE

General information: TITO was launched using: RESULT: Template: 5ULM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1466 -78290 -53.40 -226.27 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -53.40 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.234

(partial model without unconserved sides chains): PDB file : Tito_5ULM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ULM-query.scw PDB file : Tito_Scwrl_5ULM.pdb: