Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLTHYD-IPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELTAEDDSLSYEEIFRTK 2E0Q Chain:A ((39-138)) ---HLDSKNFDSFLASHEIAVVDFWAEWCAPCLILAPIIEELAEDYPQVGFGKLNSDENPDIAARYGVMSLPTVIFFKDGEPVDEIIGAVPREEIEIRIKNLL---------------

General information: TITO was launched using: RESULT: Template: 2E0Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 416 -48942 -117.65 -494.36 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -117.65 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.721

(partial model without unconserved sides chains): PDB file : Tito_2E0Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E0Q-query.scw PDB file : Tito_Scwrl_2E0Q.pdb: