Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAYYYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVARTFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKITDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDFFAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGTPNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTGVVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDADRLVEAIMATKEFFQHGTI 5J8Q Chain:A ((3-404)) ---ITDIREQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLGTRATDGYEGAREKVRKFINAKSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYMEHHANIIPWQQAVKATGATLKYIPLQEDGTISLEDVRETVTSNTKIVAVSHVSNVLGTVNPIKEMAKIAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHKMCGPTGVGVLYGKKALLENMEPAEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLGAAIDFLEEIGLDEISRHEHKLAAYALERFRQLDGVTVYGPE---ERAGLVTFNLDDVHPHDVATVLDAEGIAVRAGHH-AQPLMKWLDVTATARASFYLYNTEEEIDKLVEALQKTKEYFTN---

General information: TITO was launched using: RESULT: Template: 5J8Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2361 -252466 -106.93 -629.59 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.91 3D Compatibility (PKB) : -106.93 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_5J8Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J8Q-query.scw PDB file : Tito_Scwrl_5J8Q.pdb: