Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIINEMKKGTYGVNGEWKK 2X4K Chain:A ((3-60)) MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIEEMKPNHYGVAGVRK-

General information: TITO was launched using: RESULT: Template: 2X4K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 145 -18986 -130.93 -327.34 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -130.93 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.735

(partial model without unconserved sides chains): PDB file : Tito_2X4K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X4K-query.scw PDB file : Tito_Scwrl_2X4K.pdb: