Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFDELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGN--VQQMVE-ATYHYEKDILDRLISGQRQGIPIIEQQK---RVELLRYMK-EHQ-----IQRYSIFDLPAHLSLANYYENIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY 3V71 Chain:A ((44-145)) -------------------------------------------------------------EDQIRKAVFEKFTESTTLFVGLCHSRN-GNFIVQKLVELATPAEQRELLRQMIDG---GLLAMCKDKFACRVVQLALQKFDHSNVFQLIQELSTFDLAA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3V71.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 287 6295 21.93 75.84 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.45 3D Compatibility (PKB) : 21.93 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.45 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_3V71.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V71-query.scw PDB file : Tito_Scwrl_3V71.pdb: