Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNEYGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNIL 3VR4 Chain:H ((2-103)) -TYKIGVVGDKDSVSPFRLFGFDVQHGTTKTEIRKTIDEMAKNEYGVIYITEQCANLVPETIERYKGQLTPAIILIPSHQGTLGIGLEEIQNSVEKAVGQNIL

General information: TITO was launched using: RESULT: Template: 3VR4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 406 -58417 -143.88 -572.72 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain H : 0.93 3D Compatibility (PKB) : -143.88 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.639

(partial model without unconserved sides chains): PDB file : Tito_3VR4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VR4-query.scw PDB file : Tito_Scwrl_3VR4.pdb: