Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILYISISGNTRAFAKHLAEYAEKM-H-AEDPVNPEVTLKEIH-ENSDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK 4N82 Chain:B ((24-171)) VSLVYISLSGNTESFVRRLTDYLLEQHPSL---EVEKIHIKDLVKERQPFFEMDNPFIAFLPTYLEGGNGVDNGDVEILTTDVGDFIAYGQNASKCLGVIGSGNRNFNNQYCLTAKQYSERFGFPVLADFEMRGMLGDIKKVAGIIEELYH

General information: TITO was launched using: RESULT: Template: 4N82.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 685 -9533 -13.92 -65.74 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -13.92 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_4N82.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N82-query.scw PDB file : Tito_Scwrl_4N82.pdb: