Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERFDVERMIQMKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKENVSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSRYLMVSAVYADEPAKWP----ESMTDYYITKHYADEWL-KRTNLDFVILQPVTLTNDEEVTSIQLTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE 3DQP Chain:A ((2-185)) ------------KIFIVGSTGRVGKSLLKSLSTTDYQIYAGARKV--EQVPQYNNVKAVHFDVDWTPEEMAKQLHGMDAIINVSGSGGKSLLKVDLYGAVKLMQAAEKAEVKRFILLSTIFSLQPEKWIGAGFDALKDYYIAKHFADLYLTKETNLDYTIIQPGALTEEEATGLIDI---NDEVSASNTIGDVADTIKELV------------------------------

General information: TITO was launched using: RESULT: Template: 3DQP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 887 -87447 -98.59 -488.53 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -98.59 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_3DQP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DQP-query.scw PDB file : Tito_Scwrl_3DQP.pdb: