Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MRTLIIISHPTIEES-LNQQFFKEASAGLPATWHHLESSYPDGKIDIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERA------AHVLKGKELGIVLTTGVAEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQQYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQEMKGTSL 3EYW Chain:B ((5-411)) MRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDATRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQAGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTKARAAVYKRTSAMILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPG--FDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQARHYKQRLLEWQEAH-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3EYW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 691 -53335 -77.18 -317.47 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -77.18 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.692

(partial model without unconserved sides chains): PDB file : Tito_3EYW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EYW-query.scw PDB file : Tito_Scwrl_3EYW.pdb: