Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEILIALVPMLAWGSIGLVSGKIGGD--ANQQTLGMTIGAFLFSLVVFFVVS--PVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIAGAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIFSTIGYGVYTIIVNWA-NLDAMSIILPQSIGMILGASFFAFRKV-K-VDQFVW-KNMICGLL-WGLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVILGGILLGYMKTV 5I20 Chain:E ((6-285)) -ATLIGFTAILLWSTLALATS-STGAVPPFLLTALTFTIGGAVGIAAGLARGVGLSVLRQPWPVWVHGIGGLFGYHFFYFSALKLAPPAEAGLVAY-LWPLLIVLFSAFLPGERLRPAHV----AGALMGLAGTVVLLGARAGGFG----FAPEYVPGYLAAAACAVIWSVYSVASRRFARVPTEVVAGFCLATAALSALCHILFEPSVWPVGSEWLAVVALGIGPVGIAFYTWDIGMKRGDVRLLGVLSYAAPVLSTLLLVVAGFAAPSGAL----AIACALIVGGAAVATLLA--

General information: TITO was launched using: RESULT: Template: 5I20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1320 -176616 -133.80 -651.72 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain E : 0.66 3D Compatibility (PKB) : -133.80 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.273

(partial model without unconserved sides chains): PDB file : Tito_5I20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I20-query.scw PDB file : Tito_Scwrl_5I20.pdb: