Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNEKKAVMITKKAGEDMLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVFHPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEETGYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQQAMKDQLICDAKTIYAIQYWELLTKGK------------------------ 1MQE Chain:A ((6-207)) -------------------------FETISSETLHTGAIFALRRDQV-----RIVTREVVEHFGAVAIVAMDDNGNIPMVYQYRHTYGRRLWELPAGLLDV-AGEPPHLTAARELREEVGLQASTWQVLVDLDTAPGFSDESVRVYLATGLREVGRT----------MGWYPIAEAARRVLRGEIVNSIAIAGVLAVHAVTTGFAQPRPLDTEWIDRPTAFAARRAER

General information: TITO was launched using: RESULT: Template: 1MQE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 760 -87179 -114.71 -534.84 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -114.71 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.428

(partial model without unconserved sides chains): PDB file : Tito_1MQE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MQE-query.scw PDB file : Tito_Scwrl_1MQE.pdb: