Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVITLEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFLEYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMTEIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYYEGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKHGYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDLLITEDGNRVLTRSPKELIIL 3Q6D Chain:A ((3-356)) AMEKIERLRSAFDEAGIDGILLTNEHSRRYMANFTGTAGVVLISKKRAQFITDFRYVEQASKQAVGYEIVQHAGLIIDEVAKQVKELGIQKLGFEQDTLTYSSYSAHKEAIDAEFIPTSGLVEKLRLIKTDSEIKILKEAAQIADAAFEHILSFIRPGVSEIEVSNELEFFMRKQGATSSSFDIIVASGLRSALPHGVASEKVIETGDFVTLDFGAYYKGYCSDITRTIAVGEPSDKLKEIYNIVLEAQLRGVNGIKAGLTGREADALTRDYITEKGYGEYFGHSTGHGIGLEIHEAPGLAFRSDTVLEPGMAVTVEPGIYIPGIGGVRIEDDIIVTSEGNEVITKSPKELIIL

General information: TITO was launched using: RESULT: Template: 3Q6D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2085 -209650 -100.55 -592.23 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.90 3D Compatibility (PKB) : -100.55 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.625

(partial model without unconserved sides chains): PDB file : Tito_3Q6D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q6D-query.scw PDB file : Tito_Scwrl_3Q6D.pdb: