Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------MKVEEGQREKLKTEMERLHTYITQLSQTFYD--PDKEKVMVNYPNNS-------EGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILK------YDGKKERFIFKSVREDLPLSAGMDLEVLVEDYFTEEKHWVRTK-----LDYLPQAAGGTQASGWYITEDKELELEGVMARIRKKN------------------------------------ 5JUF Chain:A ((1-299)) MNLKDSIGLRIKTERERQQMSREVLCLDGAELTVRQLIRIEKGESLPSLDRLSYIAKRLGKSMTELLDQDNITIPDEYYEMKNRLIKFPTYRN---PDRIKSKLTLIEEVYEKFFDILPEEELLTLDILENILSFTSWEESPKVEEIYEDLFEQV-KRKRKFSTNDLLVIDYYFFHLYGRKQYDKKLFERIIKRVLNQEIWTDDVYNIVLFNDLMAIAALKIFHNSFSDFLTVVDKALAVIEKSQLYSYKPSVFVLKAKYELLHKENKKEAAENYDKAIVFASVLEDSVLEESIKAGKLADGL

General information: TITO was launched using: RESULT: Template: 5JUF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 547 31101 56.86 196.84 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 56.86 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_5JUF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JUF-query.scw PDB file : Tito_Scwrl_5JUF.pdb: