@TOME V2.3
(Nov 2016)
Ref.
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Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : BRSK2_HUMAN: (2015-10-04 )
MTSTGKDGGAQHAQYVGPYRLEKTLGKGQTGLVKLGVHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSQEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRARLNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFISLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDPPAAQHLSDTTNCMEMMTGRLSKCGSPLSNFFDVIKQLFSDEKNGQAAQAPSTPAKRSAHGPLGDSAAAGPGPGGDAEYPTGKDTAKMGPPTARREQP
Atome Classification :
(24 SA)
.........10........20........30........40........--50...-.-.-----...60........70...-.....80........90........100.......110.......120.......130.......140......---.150.---.---.....160..-.--..-..170...-....180.......190.-----......200.......210.......220.......230-....--.----..--.240-------.......250.......260.......270.......280.......290.......300..--.....310.......320.......330.......340.......350.......360.......370.......380.......390.......400.......410.......420.......430.......440.......450.......460.......470.......480.......490.......500.......510.......520.......530.......540.......550.......560.......570.......580.......590.......600.......610.......620.......630.......640.......650.......660.......670.......680.......690.......700.......710.......720.......730....
(Atome)
(Ident)
(Tito)
(Num)
(Ligand)
(Uniprot)
MTSTGKDGGA
Q
H
A
QYV
G
P
Y
R
L
E
K
T
LGKGQTG
L
V
K
L
G
V
H
C
V
T
C
Q
K
V
A
I
K
I
--
V
N
R
E
K
-
L
-
S
-----
E
S
V
L
M
K
V
E
R
E
I
A
I
L
K
L
IE-HP
H
V
L
K
L
H
D
V
YEN
K
K
Y
L
Y
L
V
L
EHVSG
G
E
L
F
D
Y
L
V
KKGRL
T
P
K
E
A
R
K
F
F
R
Q
I
I
S
A
L
D
F
C
HSH
S
I
C
HRDLKPE
N
L
L
---LDEKN---
N
---
I
R
I
A
DFG
M
A
S
-
L
--
Q
V
-
G
D
S
L
L
ETS-CGSPHYA
C
P
E
V
IRGEKYD-----GRKA
D
V
W
S
C
G
V
I
L
F
A
L
L
VGALPFD
D
D
N
L
R
Q
L
L
E
K
V
K
R
G
--VFHM--P----HF--IPP
D
-------
C
Q
S
L
L
R
G
M
I
E
V
D
A
A
R
R
L
T
L
E
H
I
Q
K
H
I
W
Y
IGGKNEPEPEQPIP
R
K
V
Q
IRSLPSLEDI
D
P
D
V
L
D
--
S
M
H
SLGC
F
R
D
R
N
K
L
L
Q
D
L
L
S
EEENQ
E
K
M
I
Y
F
L
L
LD
R
K
ERYPSQEDEDLPPRNEIDP
P
R
KRVD
S
P
M
L
N
RHGKRRPERKS
M
E
V
L
S
VTDGGSPV
P
A
R
R
A
I
E
M
A
Q
HGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGG
V
P
W
R
A
R
L
N
S
I
K
N
SFLGSPRF
H
R
R
KLQVP
T
P
E
E
M
SNLTPESS
P
E
L
A
K
K
S
WFGNFISLEKE
E
Q
I
F
V
V
IKDK
P
L
S
S
I
K
A
D
I
V
H
A
F
L
SIP
S
L
S
H
S
V
I
S
QTS
F
R
A
E
YKATGG
P
A
V
FQKP
V
K
F
Q
V
D
I
T
YTEGGEAQKEN
G
I
Y
S
V
T
F
T
LLSG
P
S
R
R
F
K
R
V
V
E
T
I
Q
A
Q
L
LSTHDP
P
A
A
Q
H
L
S
D
T
T
N
C
M
E
M
M
T
G
R
L
S
KCGS
P
L
S
N
F
F
D
V
I
K
Q
L
FSDEKNGQAAQAPSTPAKRSAHGPLGDSAAAGPGPGGDAEYPTGKDTAKMGPPTARREQP
24
PsiBlast_CBE
76.35
41%
-94
- C1 -
4CZT
4
CIPKN_ARATH
D:
[10-304]
---------------
V
G
K
Y
E
L
G
R
T
L
G
E
G
T
F
A
K
V
K
F
A
R
N
V
E
N
G
D
N
V
A
I
K
V
--
I
D
K
E
K
V
L
-
K
-----
N
K
M
I
A
Q
I
K
R
E
I
S
T
M
K
L
I
K
-
H
P
N
V
I
R
M
F
E
V
M
A
S
K
T
K
I
Y
F
V
L
E
F
V
T
G
G
E
L
F
D
K
I
S
S
N
G
R
L
K
E
D
E
A
R
K
Y
F
Q
Q
L
I
N
A
V
D
Y
C
H
S
R
G
V
Y
H
R
D
L
K
P
E
N
L
L
---
L
D
A
N
G
---
A
---
L
K
V
S
D
F
G
L
S
A
-
L
PQ
Q
V
R
E
D
G
L
L
H
T
T
-
C
G
T
P
N
Y
V
A
P
E
V
I
N
N
K
G
Y
D
-----
G
A
K
A
D
L
W
S
C
G
V
I
L
F
V
L
M
A
G
Y
L
P
F
E
D
S
N
L
T
S
L
Y
K
K
I
F
K
A
--
E
F
T
C
--
P
----
P
W
--
F
S
A
S
-------
A
K
K
L
I
K
R
I
L
D
P
N
P
A
T
R
I
T
F
A
E
V
I
E
N
E
W
F
K
K
G
Y
K
A
P
K
F
E
N
---------
A
D
V
S
L
D
D
V
D
A
I
F
D
D
--
S
----
N
L
V
V
E
R
R
E
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
25
PsiBlast_CBE
74.88
41%
-104
- C1 -
4CZT
3
CIPKN_ARATH
C:
[10-295]
---------------
V
G
K
Y
E
L
G
R
T
L
G
E
G
T
F
A
K
V
K
F
A
R
N
V
E
N
G
D
N
V
A
I
K
V
--
I
D
K
E
K
V
L
-
K
-----
N
K
M
I
A
Q
I
K
R
E
I
S
T
M
K
L
I
K
-
H
P
N
V
I
R
M
F
E
V
M
A
S
K
T
K
I
Y
F
V
L
E
F
V
T
G
G
E
L
F
D
K
I
S
S
N
G
R
L
K
E
D
E
A
R
K
Y
F
Q
Q
L
I
N
A
V
D
Y
C
H
S
R
G
V
Y
H
R
D
L
K
P
E
N
L
L
---
L
D
A
N
G
---
A
---
L
K
V
S
D
F
G
L
S
A
-
L
PQ
Q
V
R
E
D
G
L
L
H
T
T
-
C
G
T
P
N
Y
V
A
P
E
V
I
N
N
K
G
Y
D
-----
G
A
K
A
D
L
W
S
C
G
V
I
L
F
V
L
M
A
G
Y
L
P
F
E
D
S
N
L
T
S
L
Y
K
K
I
F
K
A
--
E
F
T
C
--
P
----
P
W
--
F
S
A
S
-------
A
K
K
L
I
K
R
I
L
D
P
N
P
A
T
R
I
T
F
A
E
V
I
E
N
E
W
F
K
K
G
Y
K
A
P
K
F
E
N
---------
A
D
V
S
L
D
D
V
D
A
I
F
D
D
--
S
G
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
28
PsiBlast_CBE
74.80
41%
-97
- C1 -
4CZU
3
CIPKN_ARATH
D:
[10-304]
---------------
V
G
K
Y
E
L
G
R
T
L
G
E
G
T
F
A
K
V
K
F
A
R
N
V
E
N
G
D
N
V
A
I
K
V
--
I
D
K
E
K
V
L
-
K
-----
N
K
M
I
A
Q
I
K
R
E
I
S
T
M
K
L
I
K
-
H
P
N
V
I
R
M
F
E
V
M
A
S
K
T
K
I
Y
F
V
L
E
F
V
T
G
G
E
L
F
D
K
I
S
S
N
G
R
L
K
E
D
E
A
R
K
Y
F
Q
Q
L
I
N
A
V
D
Y
C
H
S
R
G
V
Y
H
R
D
L
K
P
E
N
L
L
---
L
D
A
N
G
---
A
---
L
K
V
S
D
F
G
L
S
A
-
L
PQ
Q
V
R
E
D
G
L
L
H
D
T
-
C
G
T
P
N
Y
V
A
P
E
V
I
N
N
K
G
Y
D
-----
G
A
K
A
D
L
W
S
C
G
V
I
L
F
V
L
M
A
G
Y
L
P
F
E
D
S
N
L
T
S
L
Y
K
K
I
F
K
A
--
E
F
T
C
--
P
----
P
W
--
F
S
A
S
-------
A
K
K
L
I
K
R
I
L
D
P
N
P
A
T
R
I
T
F
A
E
V
I
E
N
E
W
F
K
K
G
Y
K
A
P
K
F
E
N
---------
A
D
V
S
L
D
D
V
D
A
I
F
D
D
--
S
----
N
L
V
V
E
R
R
E
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
18
PsiBlast_PDB
74.48
49%
-118
- C1 -
3AQV
1
AAPK2_HUMAN
A:
[10-265]
---------------
I
G
H
Y
V
L
G
D
T
L
G
V
G
T
F
G
K
V
K
I
G
E
H
Q
L
T
G
H
K
V
A
V
K
I
--
L
N
R
Q
K
-
I
R
S
-----
L
D
V
V
G
K
I
K
R
E
I
Q
N
L
K
L
F
R
-
H
P
H
I
I
K
L
Y
Q
V
I
S
T
P
T
D
F
F
M
V
M
E
Y
V
S
G
G
E
L
F
D
Y
I
C
K
H
G
R
V
E
E
M
E
A
R
R
L
F
Q
Q
I
L
S
A
V
D
Y
C
H
R
H
M
V
V
H
R
D
L
K
P
E
N
V
L
---
L
D
A
H
M
---
N
---
A
K
I
A
D
F
G
L
S
N
-
M
--
M
S
-
D
G
E
F
L
-------
P
N
Y
A
A
P
E
V
I
S
G
R
L
Y
A
-----
G
P
E
V
D
I
W
S
C
G
V
I
L
Y
A
L
L
C
G
T
L
P
F
D
D
E
H
V
P
T
L
F
K
K
I
R
G
G
--
V
F
Y
I
--
P
----
E
Y
--
L
N
R
S
-------
V
A
T
L
L
M
H
M
L
Q
V
D
P
L
K
R
A
T
I
K
D
I
R
E
H
E
W
F
K
Q
D
L
P
S
Y
L
F
P
E
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
31
PsiBlast_CBE
74.14
41%
-103
- C1 -
4CZU
3
CIPKN_ARATH
A:
[10-302]
---------------
V
G
K
Y
E
L
G
R
T
L
G
E
G
T
F
A
K
V
K
F
A
R
N
V
E
N
G
D
N
V
A
I
K
V
--
I
D
K
E
K
V
L
-
K
-----
N
K
M
I
A
Q
I
K
R
E
I
S
T
M
K
L
I
K
-
H
P
N
V
I
R
M
F
E
V
M
A
S
K
T
K
I
Y
F
V
L
E
F
V
T
G
G
E
L
F
D
K
I
S
S
N
G
R
L
K
E
D
E
A
R
K
Y
F
Q
Q
L
I
N
A
V
D
Y
C
H
S
R
G
V
Y
H
R
D
L
K
P
E
N
L
L
---
L
D
A
N
G
---
A
---
L
K
V
S
D
F
G
L
S
A
-
L
PQ
Q
V
R
E
D
G
L
L
H
D
T
-
C
G
T
P
N
Y
V
A
P
E
V
I
N
N
K
G
Y
D
-----
G
A
K
A
D
L
W
S
C
G
V
I
L
F
V
L
M
A
G
Y
L
P
F
E
D
S
N
L
T
S
L
Y
K
K
I
F
K
A
--
E
F
T
C
--
P
----
P
W
--
F
S
A
S
-------
A
K
K
L
I
K
R
I
L
D
P
N
P
A
T
R
I
T
F
A
E
V
I
E
N
E
W
F
K
K
G
Y
K
A
P
K
F
E
N
--------------
D
D
V
D
A
I
F
D
D
--
S
G
E
S
K
N
L
V
V
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
27
PsiBlast_CBE
73.94
41%
-94
- C1 -
4CZT
3
CIPKN_ARATH
A:
[10-297]
---------------
V
G
K
Y
E
L
G
R
T
L
G
E
G
T
F
A
K
V
K
F
A
R
N
V
E
N
G
D
N
V
A
I
K
V
--
I
D
K
E
K
V
L
-
K
-----
N
K
M
I
A
Q
I
K
R
E
I
S
T
M
K
L
I
K
-
H
P
N
V
I
R
M
F
E
V
M
A
S
K
T
K
I
Y
F
V
L
E
F
V
T
G
G
E
L
F
D
K
I
S
S
N
G
R
L
K
E
D
E
A
R
K
Y
F
Q
Q
L
I
N
A
V
D
Y
C
H
S
R
G
V
Y
H
R
D
L
K
P
E
N
L
L
---
L
D
A
N
G
---
A
---
L
K
V
S
D
F
G
L
S
A
-
L
PQ
Q
V
R
E
D
G
L
L
H
T
T
-
C
G
T
P
N
Y
V
A
P
E
V
I
N
N
K
G
Y
D
-----
G
A
K
A
D
L
W
S
C
G
V
I
L
F
V
L
M
A
G
Y
L
P
F
E
D
S
N
L
T
S
L
Y
K
K
I
F
K
A
--
E
F
T
C
--
P
----
P
W
--
F
S
A
S
-------
A
K
K
L
I
K
R
I
L
D
P
N
P
A
T
R
I
T
F
A
E
V
I
E
N
E
W
F
K
K
G
Y
K
A
P
K
F
E
N
-----------
V
S
L
D
D
V
D
A
I
F
D
D
--
S
G
E
S
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
29
PsiBlast_CBE
73.86
41%
-104
- C1 -
4CZU
3
CIPKN_ARATH
C:
[10-294]
---------------
V
G
K
Y
E
L
G
R
T
L
G
E
G
T
F
A
K
V
K
F
A
R
N
V
E
N
G
D
N
V
A
I
K
V
--
I
D
K
E
K
V
L
-
K
-----
N
K
M
I
A
Q
I
K
R
E
I
S
T
M
K
L
I
K
-
H
P
N
V
I
R
M
F
E
V
M
A
S
K
T
K
I
Y
F
V
L
E
F
V
T
G
G
E
L
F
D
K
I
S
S
N
G
R
L
K
E
D
E
A
R
K
Y
F
Q
Q
L
I
N
A
V
D
Y
C
H
S
R
G
V
Y
H
R
D
L
K
P
E
N
L
L
---
L
D
A
N
G
---
A
---
L
K
V
S
D
F
G
L
S
A
-
L
PQ
Q
V
R
E
D
G
L
L
H
D
T
-
C
G
T
P
N
Y
V
A
P
E
V
I
N
N
K
G
Y
D
-----
G
A
K
A
D
L
W
S
C
G
V
I
L
F
V
L
M
A
G
Y
L
P
F
E
D
S
N
L
T
S
L
Y
K
K
I
F
K
A
--
E
F
T
C
--
P
----
P
W
--
F
S
A
S
-------
A
K
K
L
I
K
R
I
L
D
P
N
P
A
T
R
I
T
F
A
E
V
I
E
N
E
W
F
K
K
G
Y
K
A
P
K
F
E
N
---------
A
D
V
-----
D
A
I
F
D
D
--
S
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
30
PsiBlast_CBE
73.22
41%
-104
- C1 -
4CZU
3
CIPKN_ARATH
B:
[10-295]
---------------
V
G
K
Y
E
L
G
R
T
L
G
E
G
T
F
A
K
V
K
F
A
R
N
V
E
N
G
D
N
V
A
I
K
V
--
I
D
K
E
K
V
L
-
K
-----
N
K
M
I
A
Q
I
K
R
E
I
S
T
M
K
L
I
K
-
H
P
N
V
I
R
M
F
E
V
M
A
S
K
T
K
I
Y
F
V
L
E
F
V
T
G
G
E
L
F
D
K
I
S
S
N
G
R
L
K
E
D
E
A
R
K
Y
F
Q
Q
L
I
N
A
V
D
Y
C
H
S
R
G
V
Y
H
R
D
L
K
P
E
N
L
L
---
L
D
A
N
G
---
A
---
L
K
V
S
D
F
G
L
S
A
-
L
PQ
Q
V
R
E
D
G
L
L
H
D
T
-
C
G
T
P
N
Y
V
A
P
E
V
I
N
N
K
G
Y
D
-----
G
A
K
A
D
L
W
S
C
G
V
I
L
F
V
L
M
A
G
Y
L
P
F
E
D
S
N
L
T
S
L
Y
K
K
I
F
K
A
--
E
F
T
C
--
P
----
P
W
--
F
S
A
S
-------
A
K
K
L
I
K
R
I
L
D
P
N
P
A
T
R
I
T
F
A
E
V
I
E
N
E
W
F
K
K
G
Y
K
A
P
K
F
E
---------------
D
D
V
D
A
I
F
D
D
--
S
G
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
26
PsiBlast_CBE
72.45
41%
-100
- C1 -
4CZT
2
CIPKN_ARATH
B:
[10-295]
---------------
V
G
K
Y
E
L
G
R
T
L
G
E
G
T
F
A
K
V
K
F
A
R
N
V
E
N
G
D
N
V
A
I
K
V
--
I
D
K
E
K
V
L
-
K
-----
N
K
M
I
A
Q
I
K
R
E
I
S
T
M
K
L
I
K
-
H
P
N
V
I
R
M
F
E
V
M
A
S
K
T
K
I
Y
F
V
L
E
F
V
T
G
G
E
L
F
D
K
I
S
S
N
G
R
L
K
E
D
E
A
R
K
Y
F
Q
Q
L
I
N
A
V
D
Y
C
H
S
R
G
V
Y
H
R
D
L
K
P
E
N
L
L
---
L
D
A
N
G
---
A
---
L
K
V
S
D
F
G
L
S
A
-
L
PQ
Q
V
R
E
D
G
L
L
H
T
T
-
C
G
T
P
N
Y
V
A
P
E
V
I
N
N
K
G
Y
D
-----
G
A
K
A
D
L
W
S
C
G
V
I
L
F
V
L
M
A
G
Y
L
P
F
E
D
S
N
L
T
S
L
Y
K
K
I
F
K
A
--
E
F
T
C
--
P
----
P
W
--
F
S
A
S
-------
A
K
K
L
I
K
R
I
L
D
P
N
P
A
T
R
I
T
F
A
E
V
I
E
N
E
W
F
K
K
G
Y
K
A
P
K
F
E
---------------
D
D
V
D
A
I
F
D
D
--
S
G
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
35
PsiBlast_CBE
72.12
39%
-115
- C1 -
4CQG
1
MELK_MOUSE
A:
[11-306]
------------------
Y
E
L
Y
E
T
I
G
T
G
G
F
A
K
V
K
L
A
C
H
V
L
T
G
E
M
V
A
I
K
I
--
M
D
K
N
A
-
L
-
G
------
S
D
L
P
R
V
K
T
E
I
D
A
L
K
S
L
R
-
H
Q
H
I
C
Q
L
Y
H
V
L
E
T
K
N
K
I
F
M
V
L
E
Y
C
P
G
G
E
L
F
D
Y
I
I
S
Q
D
R
L
S
E
E
E
T
R
V
V
F
R
Q
I
L
S
A
V
A
Y
V
H
S
Q
G
Y
A
H
R
D
L
K
P
E
N
L
L
---
F
D
E
N
H
---
K
---
L
K
L
I
D
F
G
L
C
A
-
K
--------------
C
G
S
L
A
Y
A
A
P
E
L
I
Q
----------
G
S
E
A
D
V
W
S
M
G
I
L
L
Y
V
L
M
C
G
F
L
P
F
D
D
D
N
V
M
A
L
Y
K
K
I
M
R
G
--
K
Y
E
V
--
P
----
K
W
--
L
S
P
S
-------
S
I
L
L
L
Q
Q
M
L
Q
V
D
P
K
K
R
I
S
M
R
N
L
L
N
H
P
W
V
M
Q
D
Y
S
C
P
---------
V
E
W
Q
S
K
T
P
L
T
H
L
D
E
D
C
V
T
E
L
S
V
H
H
----
R
S
S
R
Q
T
M
E
D
L
I
S
S
W
Q
Y
D
H
L
T
A
T
Y
L
L
L
L
A
K
K
A
R
L
E
H
H
H
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
105
PsiBlast_CBE
70.78
37%
-126
- C1 -
3BHH
1
KCC2B_HUMAN
A:
[6-263]
------------------
Y
Q
L
Y
E
D
I
G
----
S
V
V
R
R
C
V
K
L
C
T
G
H
E
Y
A
A
K
I
--
I
N
T
K
K
-
L
-
S
-----
A
R
D
H
Q
K
L
E
R
E
A
R
I
C
R
L
L
K
-
H
S
N
I
V
R
L
H
D
S
I
S
E
E
G
F
H
Y
L
V
F
D
L
V
T
G
G
E
L
F
E
D
I
V
A
R
E
Y
Y
S
E
A
D
A
S
H
C
I
Q
Q
I
L
E
A
V
L
H
C
H
Q
M
G
V
V
H
R
D
L
K
P
E
N
L
L
---
L
A
S
K
C
---
K
G
A
A
V
K
L
A
D
F
G
L
A
I
-
E
--
V
Q
-
G
D
Q
Q
A
W
F
G
F
A
G
T
P
G
Y
L
S
P
E
V
L
R
K
E
A
Y
------
G
K
P
V
D
I
W
A
C
G
V
I
L
Y
I
L
L
V
G
Y
P
P
F
W
D
E
D
Q
H
K
L
Y
Q
Q
I
K
A
G
--
A
Y
D
F
--
P
SPEW
D
T
--
V
T
P
E
-------
A
K
N
L
I
N
Q
M
L
T
I
N
P
A
K
R
I
T
A
H
E
A
L
K
H
P
W
V
C
Q
R
S
T
V
A
S
M
M
H
R
Q
E
T
V
E
C
L
K
K
F
N
A
R
R
K
L
K
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
104
PsiBlast_CBE
70.48
37%
-126
* C1 *
3BHH
1
KCC2B_HUMAN
B:
[6-263]
------------------
Y
Q
L
Y
E
D
I
G
K
---
S
V
V
R
R
C
V
K
L
C
T
G
H
E
Y
A
A
K
I
--
I
N
T
K
K
-
L
-
S
-----
A
R
D
H
Q
K
L
E
R
E
A
R
I
C
R
L
L
K
-
H
S
N
I
V
R
L
H
D
S
I
S
E
E
G
F
H
Y
L
V
F
D
L
V
T
G
G
E
L
F
E
D
I
V
A
R
E
Y
Y
S
E
A
D
A
S
H
C
I
Q
Q
I
L
E
A
V
L
H
C
H
Q
M
G
V
V
H
R
D
L
K
P
E
N
L
L
---
L
A
S
K
C
---
K
GAA
V
K
L
A
D
F
G
L
A
I
-
E
--
V
Q
-
G
D
Q
Q
A
W
F
G
F
A
G
T
P
G
Y
L
S
P
E
V
L
R
K
E
A
Y
------
G
K
P
V
D
I
W
A
C
G
V
I
L
Y
I
L
L
V
G
Y
P
P
F
W
D
E
D
Q
H
K
L
Y
Q
Q
I
K
A
G
--
A
Y
D
F
--
P
SPEW
D
T
--
V
T
P
E
-------
A
K
N
L
I
N
Q
M
L
T
I
N
P
A
K
R
I
T
A
H
E
A
L
K
H
P
W
V
C
Q
R
S
T
V
A
S
M
M
H
R
Q
E
T
V
E
C
L
K
K
F
N
A
R
R
K
L
K
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
62
PsiBlast_CBE
69.27
37%
-122
- C1 -
2VN9
2
KCC2D_HUMAN
A:
[6-263]
------------------
Y
Q
L
F
E
E
L
G
K
G
A
F
S
V
V
R
R
C
M
K
I
P
T
G
Q
E
Y
A
A
K
I
--
I
N
T
K
K
-
L
-
S
-----
A
R
D
H
Q
K
L
E
R
E
A
R
I
C
R
L
L
K
-
H
P
N
I
V
R
L
H
D
S
I
S
E
E
G
F
H
Y
L
V
F
D
L
V
T
G
G
E
L
F
E
D
I
V
A
R
E
Y
Y
S
E
A
D
A
S
H
C
I
Q
Q
I
L
E
S
V
N
H
C
H
L
N
G
I
V
H
R
D
L
K
P
E
N
L
L
---
L
A
S
K
S
---
K
G
A
A
V
K
L
A
D
F
G
L
A
I
-
E
--
V
Q
-
G
D
Q
Q
A
W
F
G
F
A
G
T
P
G
Y
L
S
P
E
V
L
R
K
D
P
Y
------
G
K
P
V
D
M
W
A
C
G
V
I
L
Y
I
L
L
V
G
Y
P
P
F
W
D
E
D
Q
H
R
L
Y
Q
Q
I
K
A
G
--
A
Y
D
F
--
P
SPEW
D
T
--
V
T
P
E
-------
A
K
D
L
I
N
K
M
L
T
I
N
P
A
K
R
I
T
A
S
E
A
L
K
H
P
W
I
C
Q
R
S
T
V
A
S
M
M
H
R
Q
E
T
V
D
C
L
K
K
F
N
A
R
R
K
L
K
G
A
I
L
T
--
T
M
L
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
113
PsiBlast_CBE
67.57
35%
-123
- C1 -
2Y7J
1
PHKG2_HUMAN
C:
[87-363]
---------
A
A
A
K
E
F
Y
Q
K
Y
D
P
K
D
V
I
G
R
G
V
S
S
V
V
R
R
C
V
H
R
A
T
G
H
E
F
A
V
K
I
ME
V
T
A
E
R
-
L
-
S
PEQL-
E
E
V
R
E
A
T
R
R
E
T
H
I
L
R
Q
V
A
G
H
P
H
I
I
T
L
I
D
S
Y
E
S
S
S
F
M
F
L
V
F
D
L
M
R
K
G
E
L
F
D
Y
L
T
E
K
V
A
L
S
E
K
E
T
R
S
I
M
R
S
L
L
E
A
V
S
F
L
H
A
N
N
I
V
H
R
D
L
K
P
E
N
I
L
---
L
D
D
N
M
---
Q
---
I
R
L
S
D
F
G
F
S
C
H
L
--
E
P
-
G
E
K
L
R
E
L
--
C
G
T
P
G
Y
L
A
P
E
I
L
K
C
S
M
D
E
THPGY
G
K
E
V
D
L
W
A
C
G
V
I
L
F
T
L
L
A
G
S
P
P
F
W
H
R
R
Q
I
L
M
L
R
M
I
M
E
G
--
Q
Y
---------
Q
F
--
S
S
P
E
WDDRSST
V
K
D
L
I
S
R
L
L
Q
V
D
P
E
A
R
L
T
A
E
Q
A
L
Q
H
P
F
F
E
R
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
77
PsiBlast_CBE
66.76
38%
-130
- C1 -
4FG7
1
KCC1A_HUMAN
A:
[20-277]
------------------
Y
D
F
R
D
V
L
G
T
G
A
F
S
E
V
I
L
A
E
D
K
R
T
Q
K
L
V
A
I
K
C
--
I
A
-----------------
S
M
E
N
E
I
A
V
L
H
K
I
K
-
H
P
N
I
V
A
L
D
D
I
Y
E
S
G
G
H
L
Y
L
I
M
Q
L
V
S
G
G
E
L
F
D
R
I
V
E
K
G
F
Y
T
E
R
D
A
S
R
L
I
F
Q
V
L
D
A
V
K
Y
L
H
D
L
G
I
V
H
R
D
L
K
P
E
N
L
L
YYS
L
D
E
D
S
---
K
---
I
M
I
S
D
F
G
L
S
K
-
M
--
E
D
-
P
G
---------
T
P
G
Y
V
A
P
E
V
L
A
Q
K
--------
S
K
A
V
D
C
W
S
I
G
V
I
A
Y
I
L
L
C
G
Y
P
P
F
Y
D
E
N
D
A
K
L
F
E
Q
I
L
K
A
--
E
Y
E
F
DS
P
----
Y
W
DD
I
S
D
S
-------
A
K
D
F
I
R
H
L
M
E
K
D
P
E
K
R
F
T
C
E
Q
A
L
Q
H
P
W
I
A
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
32
PsiBlast_CBE
66.09
42%
-112
- C1 -
4BL1
1
MELK_HUMAN
A:
[12-275]
------------------
Y
E
L
H
E
T
I
G
T
G
G
F
A
K
V
K
L
A
C
H
I
L
T
G
E
M
V
A
I
K
I
--
M
D
K
N
T
-
L
----------
L
P
R
I
K
T
E
I
E
A
L
K
N
L
R
-
H
Q
H
I
C
Q
L
Y
H
V
L
E
T
A
N
K
I
F
M
V
L
E
Y
C
P
G
G
E
L
F
D
Y
I
I
S
Q
D
R
L
S
E
E
E
T
R
V
V
F
R
Q
I
V
S
A
V
A
Y
V
H
S
Q
G
Y
A
H
R
D
L
K
P
E
N
L
L
---
F
D
E
Y
H
---
K
---
L
K
L
I
D
F
G
L
C
A
-
K
----------------
S
L
A
Y
A
A
P
E
L
I
Q
G
K
--------
G
S
E
A
D
V
W
S
M
G
I
L
L
Y
V
L
M
C
G
F
L
P
F
D
D
D
N
V
M
A
L
Y
K
K
I
M
R
G
--
K
Y
D
V
--
P
----
K
W
--
L
S
P
S
-------
S
I
L
L
L
Q
Q
M
L
Q
V
D
P
K
K
R
I
S
M
K
N
L
L
N
H
P
W
I
M
Q
D
Y
N
Y
P
V
E
W
Q
S
K
N
P
F
I
H
L
D
D
D
C
V
T
E
L
S
V
H
H
R
N
N
--
R
Q
T
M
E
D
L
I
S
L
W
Q
Y
D
H
L
T
A
T
Y
L
L
L
L
A
K
K
A
R
G
K
P
V
R
L
R
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
112
PsiBlast_CBE
65.53
35%
-126
- C1 -
2Y7J
1
PHKG2_HUMAN
D:
[87-363]
---------
A
A
A
K
E
F
Y
Q
K
Y
D
P
K
D
V
I
G
R
G
V
S
S
V
V
R
R
C
V
H
R
A
T
G
H
E
F
A
V
K
I
ME
V
---
R
-
L
-
S
PEQL-
E
E
V
R
E
A
T
R
R
E
T
H
I
L
R
Q
V
A
G
H
P
H
I
I
T
L
I
D
S
Y
E
S
S
S
F
M
F
L
V
F
D
L
M
R
K
G
E
L
F
D
Y
L
T
E
K
V
A
L
S
E
K
E
T
R
S
I
M
R
S
L
L
E
A
V
S
F
L
H
A
N
N
I
V
H
R
D
L
K
P
E
N
I
L
---
L
D
D
N
M
---
Q
---
I
R
L
S
D
F
G
F
S
C
H
L
--
E
P
-
G
E
K
L
R
E
L
--
C
G
T
P
G
Y
L
A
P
E
I
L
K
C
S
M
D
E
THPGY
G
K
E
V
D
L
W
A
C
G
V
I
L
F
T
L
L
A
G
S
P
P
F
W
H
R
R
Q
I
L
M
L
R
M
I
M
E
G
--
Q
Y
---------
Q
F
--
S
S
P
E
WDDRSST
V
K
D
L
I
S
R
L
L
Q
V
D
P
E
A
R
L
T
A
E
Q
A
L
Q
H
P
F
F
E
R
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
76
PsiBlast_CBE
64.91
38%
-134
- C1 -
4FG9
1
KCC1A_HUMAN
A:
[20-278]
------------------
Y
D
F
R
D
V
L
G
T
G
A
F
S
E
V
I
L
A
E
D
K
R
T
Q
K
L
V
A
I
K
C
--
I
A
K
E
-----------------
E
N
E
I
A
V
L
H
K
I
K
-
H
P
N
I
V
A
L
D
D
I
Y
E
S
G
G
H
L
Y
L
I
M
Q
L
V
S
G
G
E
L
F
D
R
I
V
E
K
G
F
Y
T
E
R
D
A
S
R
L
I
F
Q
V
L
D
A
V
K
Y
L
H
D
L
G
I
V
H
R
D
L
K
P
E
N
L
L
YYS
L
D
E
D
S
---
K
---
I
M
I
S
D
F
-----------------------
P
G
Y
V
A
P
E
V
L
A
Q
K
P
Y
------
S
K
A
V
D
C
W
S
I
G
V
I
A
Y
I
L
L
C
G
Y
P
P
F
Y
D
E
N
D
A
K
L
F
E
Q
I
L
K
A
--
E
Y
E
F
DS
P
----
Y
W
DD
I
S
D
S
-------
A
K
D
F
I
R
H
L
M
E
K
D
P
E
K
R
F
T
C
E
Q
A
L
Q
H
P
W
I
A
G
D
T
A
L
D
K
N
I
H
Q
S
V
S
E
Q
I
K
K
N
F
A
K
S
K
W
K
Q
A
F
N
A
T
--
A
V
V
R
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
103
PsiBlast_CBE
62.44
36%
-115
- C1 -
3MA6
1
?
A:
[29-290]
-------------
I
F
S
D
R
Y
K
G
Q
R
V
L
G
K
G
S
F
G
E
V
I
L
C
K
D
K
I
T
G
Q
E
C
A
V
K
V
--
I
S
K
R
Q
-
V
-
K
QKTD
K
E
S
L
L
----
R
E
V
Q
L
L
K
Q
L
D
-
H
P
N
I
M
K
L
Y
E
F
F
E
D
K
G
Y
F
Y
L
V
G
E
V
Y
T
G
G
E
L
F
D
E
I
I
S
R
K
R
F
S
E
V
D
A
A
R
I
I
R
Q
V
L
S
G
I
T
Y
M
H
K
N
K
I
V
H
R
D
L
K
P
E
N
L
L
---
L
E
S
K
S
KD
A
N
---
I
R
I
I
D
F
G
L
S
T
-
H
--
F
E
--
S
K
K
M
K
D
K
-
I
G
T
A
Y
Y
I
A
P
E
V
L
H
G
-
T
Y
D
------
E
K
C
D
V
W
S
T
G
V
I
L
Y
I
L
L
S
G
C
P
P
F
N
G
A
N
E
Y
D
I
L
K
K
V
E
K
G
KY
T
F
E
L
--
P
----
Q
W
K
K
V
S
E
S
-------
A
K
D
L
I
R
K
M
L
T
Y
V
P
S
M
R
I
S
A
R
D
A
L
D
H
E
W
I
Q
T
Y
T
K
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
75
PsiBlast_CBE
62.28
38%
-128
- C1 -
4FG9
1
KCC1A_HUMAN
B:
[20-278]
------------------
Y
D
F
R
D
V
L
G
T
G
A
F
S
E
V
I
L
A
E
D
K
R
T
Q
K
L
V
A
I
K
C
--
I
A
K
--------------------
E
I
A
V
L
H
K
I
K
-
H
P
N
I
V
A
L
D
D
I
Y
E
S
G
G
H
L
Y
L
I
M
Q
L
V
S
G
G
E
L
F
D
R
I
V
E
K
G
F
Y
T
E
R
D
A
S
R
L
I
F
Q
V
L
D
A
V
K
Y
L
H
D
L
G
I
V
H
R
D
L
K
P
E
N
L
L
YYS
L
D
E
D
S
---
K
---
I
M
I
S
D
F
--------------
V
L
S
T
A
-
C
G
T
P
G
Y
V
A
P
E
V
L
A
Q
K
P
Y
------
S
K
A
V
D
C
W
S
I
G
V
I
A
Y
I
L
L
C
G
Y
P
P
F
Y
D
E
N
D
A
K
L
F
E
Q
I
L
K
A
--
E
Y
E
F
DS
P
----
Y
W
DD
I
S
D
S
-------
A
K
D
F
I
R
H
L
M
E
K
D
P
E
K
R
F
T
C
E
Q
A
L
Q
H
P
W
I
A
G
D
T
A
L
D
K
N
I
H
Q
S
V
S
E
Q
I
K
K
N
F
A
K
S
K
W
K
Q
A
F
N
A
T
--
A
V
V
R
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
74
PsiBlast_CBE
61.73
39%
-134
- C1 -
4FG8
1
KCC1A_HUMAN
A:
[20-278]
------------------
Y
D
F
R
D
V
L
G
T
G
A
F
S
E
V
I
L
A
E
D
K
R
T
Q
K
L
V
A
I
K
C
--
I
A
K
E
------------------
N
E
I
A
V
L
H
K
I
K
-
H
P
N
I
V
A
L
D
D
I
Y
E
S
G
G
H
L
Y
L
I
M
Q
L
V
S
G
G
E
L
F
D
R
I
V
E
K
G
F
Y
T
E
R
D
A
S
R
L
I
F
Q
V
L
D
A
V
K
Y
L
H
D
L
G
I
V
H
R
D
L
K
P
E
N
L
L
YYS
L
D
E
D
S
---
K
---
I
M
I
S
D
F
----------------------
T
P
G
Y
V
A
P
E
V
L
A
Q
K
P
Y
------
S
K
A
V
D
C
W
S
I
G
V
I
A
Y
I
L
L
C
G
Y
P
P
F
Y
D
E
N
D
A
K
L
F
E
Q
I
L
K
A
EY
E
F
D
S
--
P
----
Y
W
DD
I
S
D
S
-------
A
K
D
F
I
R
H
L
M
E
K
D
P
E
K
R
F
T
C
E
Q
A
L
Q
H
P
W
I
A
G
D
T
A
L
D
K
N
I
H
Q
S
V
S
E
Q
I
K
K
N
F
A
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
73
PsiBlast_CBE
61.10
39%
-124
- C1 -
4FG8
1
KCC1A_HUMAN
B:
[20-278]
------------------
Y
D
F
R
D
V
L
G
T
G
A
F
S
E
V
I
L
A
E
D
K
R
T
Q
K
L
V
A
I
K
C
--
I
A
K
-------------------
N
E
I
A
V
L
H
K
I
K
-
H
P
N
I
V
A
L
D
D
I
Y
E
S
G
G
H
L
Y
L
I
M
Q
L
V
S
G
G
E
L
F
D
R
I
V
E
K
G
F
Y
T
E
R
D
A
S
R
L
I
F
Q
V
L
D
A
V
K
Y
L
H
D
L
G
I
V
H
R
D
L
K
P
E
N
L
L
YYS
L
D
E
D
S
---
K
---
I
M
I
S
D
F
G
-----------
G
S
V
L
S
T
A
-
C
G
T
P
G
Y
V
A
P
E
V
L
A
Q
K
P
Y
------
S
K
A
V
D
C
W
S
I
G
V
I
A
Y
I
L
L
C
G
Y
P
P
F
Y
D
E
N
D
A
K
L
F
E
Q
I
L
K
A
EY
E
F
D
S
--
P
----
Y
W
DD
I
S
D
S
-------
A
K
D
F
I
R
H
L
M
E
K
D
P
E
K
R
F
T
C
E
Q
A
L
Q
H
P
W
I
A
G
D
T
A
L
D
K
N
I
H
Q
S
V
S
E
Q
I
K
K
N
F
A
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
110
PsiBlast_CBE
59.90
34%
-103
- C1 -
2WEI
1
?
A:
[9-279]
---
S
G
R
E
N
L
Y
F
Q
G
T
F
A
E
R
Y
N
I
V
C
M
L
G
K
G
S
F
G
E
V
L
K
C
K
D
R
I
T
Q
Q
E
Y
A
V
K
V
--
I
N
K
A
S
-
A
-
K
-----
N
K
D
T
S
T
I
L
R
E
V
E
L
L
K
K
L
D
-
H
P
N
I
M
K
L
F
E
I
L
E
D
S
S
S
F
Y
I
V
G
E
L
Y
T
G
G
E
L
F
D
E
I
I
K
R
K
R
F
S
E
H
D
A
A
R
I
I
K
Q
V
F
S
G
I
T
Y
M
H
K
H
N
I
V
H
R
D
L
K
P
E
N
I
L
---
L
E
S
K
E
KD
C
D
---
I
K
I
I
D
F
G
L
S
T
-
C
--
F
Q
-
Q
N
T
K
M
K
D
R
-
I
G
T
A
Y
Y
I
A
P
E
V
L
R
G
-
T
Y
D
------
E
K
C
D
V
W
S
A
G
V
I
L
Y
I
L
L
S
G
T
P
P
F
Y
G
K
N
E
Y
D
I
L
K
R
V
E
T
G
KY
A
F
D
L
--
P
----
Q
W
RT
I
S
D
D
-------
A
K
D
L
I
R
K
M
L
T
F
H
P
S
L
R
I
T
A
T
Q
C
L
E
H
P
W
I
Q
K
Y
S
S
E
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
102
PsiBlast_CBE
58.43
36%
-114
- C1 -
3MA6
1
?
B:
[29-290]
-------------
I
F
S
D
R
Y
K
G
Q
R
V
L
G
K
G
S
F
G
E
V
I
L
C
K
D
K
I
T
G
Q
E
C
A
V
K
V
--
I
S
K
R
Q
-
V
-
K
QTDK-
E
S
L
L
----
R
E
V
Q
L
L
K
Q
L
D
-
H
P
N
I
M
K
L
Y
E
F
F
E
D
K
G
Y
F
Y
L
V
G
E
V
Y
T
G
G
E
L
F
D
E
I
I
S
R
K
R
F
S
E
V
D
A
A
R
I
I
R
Q
V
L
S
G
I
T
Y
M
H
K
N
K
I
V
H
R
D
L
K
P
E
N
L
L
---
L
E
S
K
S
KD
A
N
---
I
R
I
I
D
F
G
L
S
T
-
H
--
F
E
-
A
S
K
K
M
K
D
K
-
I
G
T
A
Y
Y
I
A
P
E
V
L
H
G
-
T
Y
D
------
E
K
C
D
V
W
S
T
G
V
I
L
Y
I
L
L
S
G
C
P
P
F
N
G
A
N
E
Y
D
I
L
K
K
V
E
K
G
KY
T
F
E
L
--
P
----
Q
W
KK
V
S
E
S
-------
A
K
D
L
I
R
K
M
L
T
Y
V
P
S
M
R
I
S
A
R
D
A
L
D
H
E
W
I
Q
T
Y
T
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------