@TOME V2.3
(Nov 2016)
Ref.
-
-
Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : bsu35000: (2016-07-29 )
MAKVRTKDVMEQFNLELISGEEGINRPITMSDLSRPGIEIAGYFTYYPRERVQLLGKTELSFFEQLPEEEKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEKNGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETALELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRSNKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMNFRLKRMGLNAAEQFTNKLADVIEDGEQEE
Atome Classification :
(20 SA)
.........10........20..-......30........40........50........60........70........80........90........100.......110.......120.......130.....-..140.......150.......160.......--170.......180.......190.......200.......210-.......220.......230.......-240.-......250.......260.......270.......-280.....-..290.......300.......310
(Atome)
(Ident)
(Tito)
(Num)
(Ligand)
(Uniprot)
MAKV
R
T
K
D
V
M
E
QFN
L
E
L
ISGEEG-INRPI
T
M
S
DLSRPG
I
E
I
A
G
Y
F
TYYP
R
E
R
V
Q
L
LG
K
T
E
L
S
F
F
E
QL
P
E
E
E
K
K
Q
R
M
D
S
L
C
TDV
T
P
A
I
I
LSRDMPI
P
Q
E
L
IDASEKNGVPVLRS
P
L
K
T
T
R
L
S
S
R
L
T
N
F
L
E
S
RLA
P
T
T
A
I
-
H
G
V
L
V
D
I
YG
V
G
V
L
I
TGKSGVGK
S
E
T
A
L
E
L
V
K
R--GHRL
V
A
D
D
C
V
E
I
R
QEDQDTLVGN
A
P
E
L
I
E
H
L
L
E
I
RGL
G
I
I
N
V
M
-
T
L
FGAGAVRSN
K
R
I
T
I
V
M
N
L
E
L
W
E
QGK-QYDR-LGL
E
E
E
T
M
K
I
I
DTE
I
T
K
L
T
I
PVRPGR
N
L
A
V
I
I
E
V
AA-M
N
F
R
L
K
RM-GL
N
A
A
E
Q
F
T
N
K
L
A
D
V
I
EDGEQEE
40
Fugue
92.31
63%
-114
- C4 -
1KO7
4
HPRK_STAXY
A:
[1-298]
--
M
L
T
T
K
S
L
V
E
R
F
E
L
E
M
I
A
G
E
A
G
-
L
N
K
Q
I
K
N
T
D
I
S
R
P
G
L
E
M
A
G
Y
F
S
H
Y
A
S
D
R
I
Q
L
L
G
T
T
E
L
S
F
Y
N
L
L
P
D
E
E
R
K
G
R
M
R
K
L
C
R
P
E
T
P
A
I
I
V
T
R
D
L
E
P
P
E
E
L
I
E
A
A
K
E
H
E
T
P
L
I
T
S
K
I
A
T
T
Q
L
M
S
R
L
T
T
F
L
E
H
E
L
A
R
T
T
S
L
-
H
G
V
L
V
D
V
Y
G
V
G
V
L
I
T
G
D
S
G
I
G
K
S
E
T
A
L
E
L
I
K
R
--
G
H
R
L
V
A
D
D
N
V
E
I
R
E
I
S
K
D
E
L
I
G
R
A
P
K
L
I
E
H
L
L
E
I
R
G
L
G
I
I
N
V
M
-
T
L
F
G
A
G
S
I
L
T
E
K
R
L
R
L
N
I
H
L
E
---------------
N
E
E
T
L
R
I
L
D
T
E
I
T
K
K
T
I
P
V
R
P
G
R
N
V
A
V
I
I
E
V
A
A
-
M
N
Y
R
L
N
I
M
-
G
I
N
T
A
E
E
F
N
D
R
L
N
----------
1
PsiBlast_PDB
91.55
63%
-115
- C4 -
1KO7
4
HPRK_STAXY
A:
[4-297]
-----
T
K
S
L
V
E
R
F
E
L
E
M
I
A
G
E
A
G
-
L
N
K
Q
I
K
N
T
D
I
S
R
P
G
L
E
M
A
G
Y
F
S
H
Y
A
S
D
R
I
Q
L
L
G
T
T
E
L
S
F
Y
N
L
L
P
D
E
E
R
K
G
R
M
R
K
L
C
R
P
E
T
P
A
I
I
V
T
R
D
L
E
P
P
E
E
L
I
E
A
A
K
E
H
E
T
P
L
I
T
S
K
I
A
T
T
Q
L
M
S
R
L
T
T
F
L
E
H
E
L
A
R
T
T
S
L
-
H
G
V
L
V
D
V
Y
G
V
G
V
L
I
T
G
D
S
G
I
G
K
S
E
T
A
L
E
L
I
K
R
--
G
H
R
L
V
A
D
D
N
V
E
I
R
E
I
S
K
D
E
L
I
G
R
A
P
K
L
I
E
H
L
L
E
I
R
G
L
G
I
I
N
V
M
-
T
L
F
G
A
G
S
I
L
T
E
K
R
L
R
L
N
I
H
L
E
---------------
N
E
E
T
L
R
I
L
D
T
E
I
T
K
K
T
I
P
V
R
P
G
R
N
V
A
V
I
I
E
V
A
A
-
M
N
Y
R
L
N
I
M
-
G
I
N
T
A
E
E
F
N
D
R
L
-----------
14
PsiBlast_CBE
90.71
63%
-119
- C4 -
1KO7
4
HPRK_STAXY
B:
[4-297]
-----
T
K
S
L
V
E
R
F
E
L
E
M
I
A
G
E
A
G
-
L
N
K
Q
I
K
N
T
D
I
S
R
P
G
L
E
M
A
G
Y
F
S
H
Y
A
S
D
R
I
Q
L
L
G
T
T
E
L
S
F
Y
N
L
L
P
D
E
E
R
K
G
R
M
R
K
L
C
R
P
E
T
P
A
I
I
V
T
R
D
L
E
P
P
E
E
L
I
E
A
A
K
E
H
E
T
P
L
I
T
S
K
I
A
T
T
Q
L
M
S
R
L
T
T
F
L
E
H
E
L
A
R
T
T
S
L
-
H
G
V
L
V
D
V
Y
G
V
G
V
L
I
T
G
D
S
G
I
G
K
S
E
T
A
L
E
L
I
K
R
--
G
H
R
L
V
A
D
D
N
V
E
I
R
E
I
S
K
D
E
L
I
G
R
A
P
K
L
I
E
H
L
L
E
I
R
G
L
G
I
I
N
V
M
-
T
L
F
G
A
G
S
I
L
T
E
K
R
L
R
L
N
I
H
L
E
---------------
N
E
E
T
L
R
I
L
D
T
E
I
T
K
K
T
I
P
V
R
P
G
R
N
V
A
V
I
I
E
V
A
A
-
M
N
Y
R
L
N
I
M
-
G
I
N
T
A
E
E
F
N
D
R
L
-----------
20
HHSearch
79.69
61%
9
- C4 -
1KO7
4
HPRK_STAXY
A:
[1-298]
--
M
L
T
T
K
S
L
V
E
R
F
E
L
E
M
I
A
G
E
-
A
G
L
N
K
Q
I
K
N
T
D
I
S
R
P
G
L
E
M
A
G
Y
F
S
H
Y
A
S
D
R
I
Q
L
L
G
T
T
E
L
S
F
Y
N
L
L
P
D
E
E
R
K
G
R
M
R
K
L
C
R
P
E
T
P
A
I
I
V
T
R
D
L
E
P
P
E
E
L
I
E
A
A
K
E
H
E
T
P
L
I
T
S
K
I
A
T
T
Q
L
M
S
R
L
T
T
F
L
E
H
E
L
A
R
T
T
S
L
-
H
G
V
L
V
D
V
Y
G
V
G
V
L
I
T
G
D
S
G
I
G
K
S
E
T
A
L
E
L
I
K
R
--
G
H
R
L
V
A
D
D
N
V
E
I
R
E
I
S
K
D
E
L
I
G
R
A
P
K
L
I
E
H
L
L
E
I
R
G
L
G
I
I
N
V
M
-
T
L
F
G
A
G
S
I
L
T
E
K
R
L
R
L
N
I
H
L
E
---------------
N
E
E
T
L
R
I
L
D
T
E
I
T
K
K
T
I
P
V
R
P
G
R
N
V
A
V
I
I
E
V
A
A
-
M
N
Y
R
L
N
I
M
-
G
I
N
T
A
E
E
F
N
D
R
L
N
----------
18
PsiBlast_CBE
72.32
59%
-156
- C4 -
1KKL
1
HPRK_LACCA
B:
[21-195]
-------------------------------------------------------------------------------------------------------------------------------------
E
R
R
S
M
-
H
G
V
L
V
D
I
Y
G
L
G
V
L
I
T
G
D
S
G
V
G
K
S
E
T
A
L
E
L
V
Q
R
--
G
H
R
L
I
A
D
D
R
V
D
V
Y
Q
Q
D
E
Q
T
I
V
G
A
A
P
P
I
L
S
H
L
L
E
I
R
G
L
G
I
I
D
V
M
-
N
L
F
G
A
G
A
V
R
E
D
T
T
I
S
L
I
V
H
L
E
N
W
T
P
-----------
G
E
Q
T
Q
L
I
F
D
V
P
V
P
K
I
T
V
P
V
K
V
G
R
N
L
A
I
I
I
E
V
A
A
-
M
N
F
R
A
K
S
M
-
G
Y
D
A
T
K
T
F
E
K
N
L
N
H
L
I
E
H
N
E
---
41
Fugue
72.01
72%
-135
- C4 -
1KO7
2
HPRK_STAXY
A:
[131-298]
-------------------------------------------------------------------------------------------------------------------------------------
R
T
T
S
L
-
H
G
V
L
V
D
V
Y
G
V
G
V
L
I
T
G
D
S
G
I
G
K
S
E
T
A
L
E
L
I
K
R
--
G
H
R
L
V
A
D
D
N
V
E
I
R
E
I
S
K
D
E
L
I
G
R
A
P
K
L
I
E
H
L
L
E
I
R
G
L
G
I
I
N
V
M
-
T
L
F
G
A
G
S
I
L
T
E
K
R
L
R
L
N
I
H
L
E
-------------
N
--
E
E
T
L
R
I
L
D
T
E
I
T
K
K
T
I
P
V
R
P
G
R
N
V
A
V
I
I
E
V
A
A
-
M
N
Y
R
L
N
I
M
-
G
I
N
T
A
E
E
F
N
D
R
L
N
----------
2
PsiBlast_PDB
71.91
59%
-151
- C4 -
1KKL
1
HPRK_LACCA
A:
[21-196]
-------------------------------------------------------------------------------------------------------------------------------------
E
R
R
S
M
-
H
G
V
L
V
D
I
Y
G
L
G
V
L
I
T
G
D
S
G
V
G
K
S
E
T
A
L
E
L
V
Q
R
--
G
H
R
L
I
A
D
D
R
V
D
V
Y
Q
Q
D
E
Q
T
I
V
G
A
A
P
P
I
L
S
H
L
L
E
I
R
G
L
G
I
I
D
V
M
-
N
L
F
G
A
G
A
V
R
E
D
T
T
I
S
L
I
V
H
L
E
N
W
T
P
D
-----------
E
Q
T
Q
L
I
F
D
V
P
V
P
K
I
T
V
P
V
K
V
G
R
N
L
A
I
I
I
E
V
A
A
-
M
N
F
R
A
K
S
M
-
G
Y
D
A
T
K
T
F
E
K
N
L
N
H
L
I
E
H
N
E
E
--
17
PsiBlast_CBE
71.39
59%
-153
- C4 -
1KKL
1
HPRK_LACCA
C:
[20-196]
------------------------------------------------------------------------------------------------------------------------------------
A
E
R
R
S
M
-
H
G
V
L
V
D
I
Y
G
L
G
V
L
I
T
G
D
S
G
V
G
K
S
E
T
A
L
E
L
V
Q
R
--
G
H
R
L
I
A
D
D
R
V
D
V
Y
Q
Q
D
E
Q
T
I
V
G
A
A
P
P
I
L
S
H
L
L
E
I
R
G
L
G
I
I
D
V
M
-
N
L
F
G
A
G
A
V
R
E
D
T
T
I
S
L
I
V
H
L
E
N
W
T
P
D
-----------
E
Q
T
Q
L
I
F
D
V
P
V
P
K
I
T
V
P
V
K
V
G
R
N
L
A
I
I
I
E
V
A
A
-
M
N
F
R
A
K
S
M
-
G
Y
D
A
T
K
T
F
E
K
N
L
N
H
L
I
E
H
N
E
E
--
21
HHSearch
68.67
59%
-149
- C4 -
2QMH
-
HPRK_LACCA
A:
[135-308]
-------------------------------------------------------------------------------------------------------------------------------------
E
R
R
S
M
-
H
G
V
L
V
D
I
Y
G
L
G
V
L
I
T
G
D
S
G
V
G
K
S
E
T
A
L
E
L
V
Q
R
--
G
H
R
L
I
A
D
D
R
V
D
V
Y
Q
Q
D
E
Q
T
I
V
G
A
A
P
P
I
L
S
H
L
L
E
I
R
G
L
G
I
I
D
V
M
-
N
L
F
G
A
G
A
V
R
E
D
T
T
I
S
L
I
V
H
L
E
---------------
G
E
Q
T
Q
L
I
F
D
V
P
V
P
K
I
T
V
P
F
K
V
G
R
N
L
A
I
I
I
E
V
A
A
-
M
N
F
R
A
K
S
M
-
G
Y
D
A
T
K
T
F
E
K
N
L
N
H
L
I
E
H
N
----
4
PsiBlast_PDB
68.48
58%
-149
- C4 -
2QMH
-
HPRK_LACCA
A:
[135-308]
-------------------------------------------------------------------------------------------------------------------------------------
E
R
R
S
M
-
H
G
V
L
V
D
I
Y
G
L
G
V
L
I
T
G
D
S
G
V
G
K
S
E
T
A
L
E
L
V
Q
R
--
G
H
R
L
I
A
D
D
R
V
D
V
Y
Q
Q
D
E
Q
T
I
V
G
A
A
P
P
I
L
S
H
L
L
E
I
R
G
L
G
I
I
D
V
M
-
N
L
F
G
A
G
A
V
R
E
D
T
T
I
S
L
I
V
H
L
E
---------------
G
E
Q
T
Q
L
I
F
D
V
P
V
P
K
I
T
V
P
F
K
V
G
R
N
L
A
I
I
I
E
V
A
A
-
M
N
F
R
A
K
S
M
-
G
Y
D
A
T
K
T
F
E
K
N
L
N
H
L
I
E
H
N
----
15
PsiBlast_CBE
67.92
59%
-142
- C4 -
1KKM
2
HPRK_LACCA
C:
[22-196]
--------------------------------------------------------------------------------------------------------------------------------------
R
R
S
M
-
H
G
V
L
V
D
I
Y
G
L
G
V
L
I
T
G
D
S
G
V
G
K
S
E
T
A
L
E
L
V
Q
R
--
G
H
R
L
I
A
D
D
R
V
D
V
Y
Q
Q
D
E
Q
T
I
V
G
A
A
P
P
I
L
S
H
L
L
E
I
R
G
L
G
I
I
D
V
M
-
N
L
F
G
A
G
A
V
R
E
D
T
T
I
S
L
I
V
H
L
E
N
W
T
P
D
K
-
T
F
D
R
-
L
G
S
G
E
Q
T
Q
L
I
F
D
V
P
V
P
K
I
T
V
P
V
K
V
G
R
N
L
A
I
I
I
E
V
A
A
-
M
N
F
R
A
K
S
M
-
G
Y
D
A
T
K
T
F
E
K
N
L
N
H
L
I
E
H
N
E
E
--
3
PsiBlast_PDB
67.55
59%
-137
- C4 -
1KKM
2
HPRK_LACCA
A:
[21-196]
-------------------------------------------------------------------------------------------------------------------------------------
E
R
R
S
M
-
H
G
V
L
V
D
I
Y
G
L
G
V
L
I
T
G
D
S
G
V
G
K
S
E
T
A
L
E
L
V
Q
R
--
G
H
R
L
I
A
D
D
R
V
D
V
Y
Q
Q
D
E
Q
T
I
V
G
A
A
P
P
I
L
S
H
L
L
E
I
R
G
L
G
I
I
D
V
M
-
N
L
F
G
A
G
A
V
R
E
D
T
T
I
S
L
I
V
H
L
E
N
W
T
P
D
K
-
T
F
D
R
-
L
G
S
G
E
Q
T
Q
L
I
F
D
V
P
V
P
K
I
T
V
P
V
K
V
G
R
N
L
A
I
I
I
E
V
A
A
-
M
N
F
R
A
K
S
M
-
G
Y
D
A
T
K
T
F
E
K
N
L
N
H
L
I
E
H
N
E
E
--
16
PsiBlast_CBE
66.78
59%
-137
- C4 -
1KKM
3
HPRK_LACCA
B:
[21-197]
-------------------------------------------------------------------------------------------------------------------------------------
E
R
R
S
M
-
H
G
V
L
V
D
I
Y
G
L
G
V
L
I
T
G
D
S
G
V
G
K
S
E
T
A
L
E
L
V
Q
R
--
G
H
R
L
I
A
D
D
R
V
D
V
Y
Q
Q
D
E
Q
T
I
V
G
A
A
P
P
I
L
S
H
L
L
E
I
R
G
L
G
I
I
D
V
M
-
N
L
F
G
A
G
A
V
R
E
D
T
T
I
S
L
I
V
H
L
E
N
W
T
P
D
K
-
T
F
D
R
-
L
G
S
G
E
Q
T
Q
L
I
F
D
V
P
V
P
K
I
T
V
P
V
K
V
G
R
N
L
A
I
I
I
E
V
A
A
-
M
N
F
R
A
K
S
M
-
G
Y
D
A
T
K
T
F
E
K
N
L
N
H
L
I
E
H
N
E
E
T
-
5
PsiBlast_PDB
65.23
57%
-149
- C4 -
1JB1
1
HPRK_LACCA
A:
[21-193]
-------------------------------------------------------------------------------------------------------------------------------------
E
R
R
S
M
-
H
G
V
L
V
D
I
Y
G
L
G
V
L
I
T
G
D
S
G
V
G
K
S
E
T
A
L
E
L
V
Q
R
--
G
H
R
L
I
A
D
D
R
V
D
V
Y
Q
Q
D
E
Q
T
I
V
G
A
A
P
P
I
L
S
H
L
L
E
I
R
G
L
G
I
I
D
V
M
-
N
L
F
G
A
G
A
V
R
E
D
T
T
I
S
L
I
V
H
L
E
N
W
T
P
D
--------------
Q
L
I
F
D
V
P
V
P
K
I
T
V
P
V
K
V
G
R
N
L
A
I
I
I
E
V
A
A
-
M
N
F
R
A
K
S
M
-
G
Y
D
A
T
K
T
F
E
K
N
L
N
H
L
I
E
H
-----
22
HHSearch
57.38
33%
-110
- C4 -
3TQF
1
?
A:
[4-168]
--------------------------------------------------------------------------------------------------------------------------------------
K
Q
T
W
-
H
A
N
F
L
V
I
D
K
M
G
V
L
I
T
G
E
A
N
I
G
K
S
E
L
S
L
A
L
I
D
R
--
G
H
Q
L
V
C
D
D
V
I
D
L
K
Q
E
N
-
N
Q
L
I
G
S
C
P
S
V
A
N
G
Y
I
L
I
T
G
I
G
I
I
D
V
P
-
K
L
F
G
L
D
A
V
V
N
Q
H
E
V
H
L
S
I
S
L
V
K
P
E
K
M
P
L
L
D
D
P
-
L
N
P
L
Y
R
T
E
I
I
L
G
I
N
V
P
K
I
L
F
P
I
H
P
G
R
N
L
P
L
L
I
E
T
L
V
-
R
N
H
R
L
K
M
E
-
G
Y
D
S
S
H
H
F
H
E
H
------------
6
PsiBlast_PDB
57.32
36%
-110
- C4 -
3TQF
1
?
A:
[8-165]
-------------------------------------------------------------------------------------------------------------------------------------------
H
A
N
F
L
V
I
D
K
M
G
V
L
I
T
G
E
A
N
I
G
K
S
E
L
S
L
A
L
I
D
R
--
G
H
Q
L
V
C
D
D
V
I
D
L
K
Q
E
N
-
N
Q
L
I
G
S
C
P
S
V
A
N
G
Y
I
L
I
T
G
I
G
I
I
D
V
P
-
K
L
F
G
L
D
A
V
V
N
Q
H
E
V
H
L
S
I
S
L
V
K
P
E
K
M
P
-
L
L
D
D
P
L
N
P
L
Y
R
T
E
I
I
L
G
I
N
V
P
K
I
L
F
P
I
H
P
G
R
N
L
P
L
L
I
E
T
L
V
-
R
N
H
R
L
K
M
E
-
G
Y
D
S
S
H
H
F
---------------
42
Fugue
51.39
57%
-34
- C4 -
1JB1
1
HPRK_LACCA
A:
[21-193]
--------------------------------------------------------------------------------------------------------------------------------------
E
R
R
S
M
H
G
V
L
V
D
I
Y
G
L
G
V
L
I
T
G
D
S
G
V
G
K
S
E
T
A
L
E
L
V
Q
R
--
G
H
R
L
I
A
D
D
R
V
D
V
Y
Q
Q
D
E
Q
T
I
V
G
A
A
P
P
I
L
S
H
L
L
E
I
R
G
L
G
I
I
D
V
-M
N
L
F
G
A
G
A
V
R
E
D
T
T
I
S
L
I
V
H
L
E
N
W
T
P
D
Q
--------------
L
I
F
D
V
P
V
P
K
I
T
V
P
V
K
V
G
R
N
L
A
I
I
I
E
V
A
A
M
N
--
F
R
A
K
S
M
G
Y
D
A
T
K
T
F
E
K
N
L
N
H
L
I
E
H
-----
39
HHSearch
38.80
33%
-126
- C4 -
2BDT
1
?
A:
[3-41]
----------------------------------------------------------------------------------------------------------------------------------------------------
K
L
Y
I
I
T
G
P
A
G
V
G
K
S
T
T
C
K
R
L
A
A
Q
LD
N
S
A
Y
I
E
G
D
I
I
----------------------------------------------------------------------------------------------------------------------------------------
35
HHSearch
37.17
24%
-126
- C4 -
1E6C
4
AROL_DICCH
A:
[3-37]
----------------------------------------------------------------------------------------------------------------------------------------------------
E
P
I
F
M
V
G
A
R
G
C
G
M
T
T
V
G
R
E
L
A
R
A
L
-
G
Y
E
F
V
D
T
D
I
F
M
---------------------------------------------------------------------------------------------------------------------------------------
38
HHSearch
35.16
24%
-107
- C4 -
3TRF
1
AROK_COXBU
A:
[5-38]
---------------------------------------------------------------------------------------------------------------------------------------------------
L
T
N
I
Y
L
I
G
L
M
G
A
G
K
T
S
V
G
S
Q
L
A
K
L
T
-
K
R
I
L
Y
D
S
D
K
-----------------------------------------------------------------------------------------------------------------------------------------