Query sequence : PF00069_CDK19_: (2014-08-15 ) MDYDFKAKLAAERERVEDLFEYEGCKVGRGTYGHVYKARRKDGKDEKEYALKQIEGTGISMSACREIALLRELKHPNVIALQKVFLSHSDRKVWLLFDYAEHDLWHIIKFHRASKANKKPMQLPRSMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPFHHDQLDRIFSVMGFPADKDWEDIRKMPEYPTLQKDFRRTTYANSSLIKYMEKHKVKPDSKVFLLLQKLLTMDPTKRITSEQALQDPYFQEDPLPTLDVFAGCQIPYPKREFLNEDDPEEKGDKNQQQQQNQHQQPTAPPQQAAAPPQAPPPQQNSTQTNGTAGGAGAGVGGTGAGLQHSQDSSLNQVPPNKKPRLGPSGANSGGPVMPSDYQHSSSRLNYQSSVQGSSQSQSTLGYSSSSQQSSQYHPSHQAHRY

Atome Classification :

	(23 SA)										.........10........20........30........40........50...----....---.----60---.........70........80.......----.---90---.........100.......110.......120.......130.......140.......150.......160.......170.......180.-......190.......200.......210.......220.......-----------230......---.240....-...250.......260.......270....-...280....--.--..290........300..--.....310.......320.......330.....--..340.......350.......360.......370.......380.......390.......400.......410.......420.......430.......440.......450.......460.......470.......480.......490.......500
			(Atome)	(Ident)	(Tito)	(Num)		(Ligand)	(Uniprot)		MDYDFKAKLAAERERVEDLFEYEGCKVGRGTYGHVYKARRKDGKDEKEYALKQI----EGTG---I----S----MSACREIALLRELKHPNVIALQKVFLSH----S---D----RKVWLLFDYAEHDLWHIIKFHRASKANKKPMQLPRSMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSP-LKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLT-----------SEPIFHCRQ---EDIKTSNP-FHHDQLDRIFSVMGFPADKDWEDIRKMPEY-PTLQKDFRRT--T--YAN-SSLIKYMEKHKVKP--DSKVFLLLQKLLTMDPTKRITSEQALQDPYFQE--DPLPTLDVFAGCQIPYPKREFLNEDDPEEKGDKNQQQQQNQHQQPTAPPQQAAAPPQAPPPQQNSTQTNGTAGGAGAGVGGTGAGLQHSQDSSLNQVPPNKKPRLGPSGANSGGPVMPSDYQHSSSRLNYQSSVQGSSQSQSTLGYSSSSQQSSQYHPSHQAHRY
2	PsiBlast_PDB		96.86	87%	-124	- C1 -	4F6S	6	CDK8_HUMAN	A:[3-361]	MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQI----EGTG---I----S----MSACREIALLRELKHPNVISLQKVFLSH----A---D----RKVWLLFDYAEHDLWHIIKFHRAS--------LPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFA------------------VTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLT-----------SEPIFHCR----------NP-YHHDQLDRIFNVMGFPADKDWEDIKKMPEH-STLMKDFRRN--T--YTN-CSLIKYMEKHKVKP--DSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLE--DPLPTSDVFAGCQIPYPKREFL-----------------------------------------------------------------------------------------------------------------------------------------------
9	PsiBlast_PDB		96.77	87%	-123	- C1 -	4F7S	8	CDK8_HUMAN	A:[3-361]	MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQI----EGTG---I----S----MSACREIALLRELKHPNVISLQKVFLSH----A---D----RKVWLLFDYAEHDLWHIIKFHRASK------QLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSP-LKP---------TFWYRAPELLLGARHYTKAIDIWAIGCIFAELLT-----------SEPIFHCR--------TSNP-YHHDQLDRIFNVMGFPADKDWEDIKKMPEH-STLMKDFRRN--T--YTN-CSLIKYMEKHKVKP--DSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLE--DPLPTSDVFAGCQIPYPKREFL-----------------------------------------------------------------------------------------------------------------------------------------------
8	PsiBlast_PDB		96.43	87%	-125	- C1 -	4F7N	2	CDK8_HUMAN	A:[3-363]	MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQI----EGTG---I----S----MSACREIALLRELKHPNVISLQKVFLSH----A---D----RKVWLLFDYAEHDLWHIIKFHRAS------VQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGF------------------VVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLT-----------SEPIFHCRQ---E-----NP-YHHDQLDRIFNVMGFPADKDWEDIKKMPEH-STLMKDFRRN--T--YTN-CSLIKYMEKHKVKP--DSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLE--DPLPTSDVFAGCQIPYPKREFLTE---------------------------------------------------------------------------------------------------------------------------------------------
4	PsiBlast_PDB		96.43	87%	-121	- C1 -	4F6W	10	CDK8_HUMAN	A:[3-361]	MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQI----EGTG---I----S----MSACREIALLRELKHPNVISLQKVFLSH----A---D----RKVWLLFDYAEHDLWHIIKFHRASK-----VQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFA-------------------TFWYRAPELLLGARHYTKAIDIWAIGCIFAELLT-----------SEPIFHCRQ---------NP-YHHDQLDRIFNVMGFPADKDWEDIKKMPEH-STLMKDFRRN--T--YTN-CSLIKYMEKHKVKP--DSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLE--DPLPTSDVFAGCQIPYPKREFL-----------------------------------------------------------------------------------------------------------------------------------------------
31	HHSearch		96.34	91%	-122	- C1 -	4F6U	10	CDK8_HUMAN	A:[3-361]	MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQI----EGTG---I----S----MSACREIALLRELKHPNVISLQKVFLSH----A---D----RKVWLLFDYAEHDLWHIIKFHRASK-----VQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGF------------------VVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLT-----------SEPIFHCRQ---E-----NP-YHHDQLDRIFNVMGFPADKDWEDIKKMPEH-STLMKDFRRN--T--YTN-CSLIKYMEKHKVKP--DSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLE--DPLPTSDVFAGCQIPYPKREFL-----------------------------------------------------------------------------------------------------------------------------------------------
5	PsiBlast_PDB		96.20	87%	-126	- C1 -	4F70	1	CDK8_HUMAN	A:[3-361]	MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQI----EGTG---I----S----MSACREIALLRELKHPNVISLQKVFLSH----A---D----RKVWLLFDYAEHDLWHIIKFHRASKA------LPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADM---------------------VTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLT-----------SEPIFHCR----------NP-YHHDQLDRIFNVMGFPADKDWEDIKKMPEH-STLMKDFRRN--T--YTN-CSLIKYMEKHKVKP--DSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLE--DPLPTSDVFAGCQIPYPKREFL-----------------------------------------------------------------------------------------------------------------------------------------------
7	PsiBlast_PDB		95.12	87%	-122	- C1 -	4F7L	1	CDK8_HUMAN	A:[3-361]	MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQI----EGTG---I----S----MSACREIALLRELKHPNVISLQKVFLSH----A---D----RKVWLLFDYAEHDLWHIIKFHRAS-------QLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMG---------------------TFWYRAPELLLGARHYTKAIDIWAIGCIFAELLT-----------SEPIFHCRQ---------NP-YHHDQLDRIFNVMGFPADKDWEDIKKMPEH-STLMKDFRRN--T--YTN-CSLIKYMEKHKVKP--DSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLE--DPLPTSDVFAGCQIPYPKREFL-----------------------------------------------------------------------------------------------------------------------------------------------
1	PsiBlast_PDB		95.00	87%	-130	- C1 -	3RGF	6	CDK8_HUMAN	A:[3-355]	MDYDFKVKLSSERERVEDLFEYEGCKVG-----HVYKAKRKDGKDDKDYALKQI----EGTG---I----S----MSACREIALLRELKHPNVISLQKVFLSH----A---D----RKVWLLFDYAEHDLWHIIKFHRASK-------LPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFAR-----------------VTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLT-----------SEPIFHCRQ---------NP-YHHDQLDRIFNVMGFPADKDWEDIKKMPEH-STLMKDFRRN--T--YTN-CSLIKYMEKHKVKP--DSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLE--DPLPTSDVFAGCQIPY-----------------------------------------------------------------------------------------------------------------------------------------------------
6	PsiBlast_PDB		93.87	87%	-126	- C1 -	4F7J	3	CDK8_HUMAN	A:[3-361]	MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQI----EGTG---I----S----MSACREIALLRELKHPNVISLQKVFLSH----A---D----RKVWLLFDYAEHDLWHIIKFHR---------QLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGF-------------------VTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLT-----------SEPIFHCRQ---------NP-YHHDQLDRIFNVMGFPADKDWEDIKKMPEH-STLMKDFRRN--T--YTN-CSLIKYMEKHKVKP--DSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLE--DPLPTSDVFAGCQIPYPKREFL-----------------------------------------------------------------------------------------------------------------------------------------------
34	HHSearch		64.36	36%	-114	- C1 -	3N9X	2	?	A:[31-384]	-----------------------KHLIGRGSYGYVYLAYDKN--TEKNVAIKKV----NRMF---E----DLIDCKRILREITILNRLKSDYIIRLYDLIIPD----D---LLKF-DELYIVLEIADSDLKKLFKT---------PIFLTEEHIKTILYNLLLGENFIHESGIIHRDLKPANCLLNQ----DCSVKVCDFGLARTI-------------HVVTRWYRAPELILLQENYTKSIDIWSTGCIFAELLNMLQSHINDPTNRFPLFPGSSCFP------HEKSNRDQLNIIFNIIGTPTEDDLKNINKP-EV-IKYIKLFPH------RKP-INLKQKYP----SI--SDDGINLLESMLKFNPNKRITIDQALDHPYLKD--VRKKKLE---TKKIILP----------------------------------------------------------------------------------------------------------------------------------------------------
17	PsiBlast_PDB		63.54	40%	-103	- C1 -	3PY0	2	CDK2_HUMAN	A:[17-304]	-------------------------KIGEGTYGVVYKARNKLTGEV--VALKKIR----TEG---V----P----STAIREISLLKELNHPNIVKLLDVI--H----T---E----NKLYLVFEFLHQDL---KKFMDASALTGIPLPL----IKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGA----IKLADFGLARAFGVP-VRTYTH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-----------RRALFPGDS---EI----------DQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQ-----------------DFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL---------------------------------------------------------------------------------------------------------------------------------------------------------------
20	PsiBlast_PDB		63.24	40%	-104	- C1 -	3QQF	2	CDK2_HUMAN	A:[17-304]	-------------------------KIGEGTYGVVYKARNKLTGEV--VALKKIR----TEG---V----P----STAIREISLLKELNHPNIVKLLDVI--H----T---E----NKLYLVFEFLHQDL---KKFMDASALTGIPLPL----IKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGA----IKLADFGLARAFGVP-VRTYTH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-----------RRALFPGDS---EI----------DQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQ-----------------DFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL---------------------------------------------------------------------------------------------------------------------------------------------------------------
12	PsiBlast_PDB		63.05	40%	-102	- C1 -	3PXF	7	CDK2_HUMAN	A:[17-304]	-------------------------KIGEGTYGVVYKARNKLTGEV--VALKKIRLDTETEG---V----P----STAIREISLLKELNHPNIVKLLDVI--H----T---E----NKLYLVFEFLHQDL---KKFMDASALTGIPLPL----IKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGA----IKLADFGLARAFGVP-VRTYTH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-----------RRALFPGDS---EI----------DQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQ-----------------DFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL---------------------------------------------------------------------------------------------------------------------------------------------------------------
19	PsiBlast_PDB		62.99	40%	-103	- C1 -	3QL8	2	CDK2_HUMAN	A:[17-304]	-------------------------KIGEGTYGVVYKARNKLTGEV--VALKKIR----TEG---V----P----STAIREISLLKELNHPNIVKLLDVI--H----T---E----NKLYLVFEFLHQDL---KKFMDASALTGIPLPL----IKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGA----IKLADFGLARAFGVP-VRTYTH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-----------RRALFPGDS---EI----------DQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQ-----------------DFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL---------------------------------------------------------------------------------------------------------------------------------------------------------------
16	PsiBlast_PDB		62.91	40%	-101	- C1 -	3PXZ	3	CDK2_HUMAN	A:[17-304]	-------------------------KIGEGTYGVVYKARNKLTGEV--VALKKIRLDTETEG---V----P----STAIREISLLKELNHPNIVKLLDVI--H----T---E----NKLYLVFEFLHQDL---KKFMDASALTGIPLPL----IKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGA----IKLADFGLARAFGVP-VRTYTH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-----------RRALFPGDS---EI----------DQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQ-----------------DFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL---------------------------------------------------------------------------------------------------------------------------------------------------------------
15	PsiBlast_PDB		62.67	40%	-100	- C1 -	3PXY	1	CDK2_HUMAN	A:[17-304]	-------------------------KIGEGTYGVVYKARNKLTGEV--VALKKIR----TEG---V----P----STAIREISLLKELNHPNIVKLLDVI--H----T---E----NKLYLVFEFLHQDL---KKFMDASALTGIPLPL----IKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGA----IKLADFGLARAFGVP-VRTYTH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-----------RRALFPGDS---EI----------DQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQ-----------------DFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL---------------------------------------------------------------------------------------------------------------------------------------------------------------
11	PsiBlast_PDB		62.33	40%	-110	- C1 -	2IW8	1	CDK2_HUMAN	A:[13-300]	-------------------------KIGEGTYGVVYKARNKLTGEV--VALKKIRLDTETEG---V----P----STAIREISLLKELNHPNIVKLLDVI--H----T---E----NKLYLVFEHVDQDL---KKFMDASALTGIPLPL----IKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGA----IKLADFGLARAFGVP-VRTYTH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-----------RRALFPGDS---EI----------DQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQ-----------------DFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL---------------------------------------------------------------------------------------------------------------------------------------------------------------
13	PsiBlast_PDB		60.23	40%	-98	- C1 -	3PXQ	4	CDK2_HUMAN	A:[17-304]	-------------------------KIGEGTYGVVYKARNKLTGEV--VALKKIR----TEG---V----P----STAIREISLLKELNHPNIVKLLDVI--H----T---E----NKLYLVFEFLHQDL---KKFMDASALTGIPLPL----IKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGA----IKLADFGLARAFGVP-VRTYTH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-----------RRALFPGDS---EI----------DQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQ-----------------DFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL---------------------------------------------------------------------------------------------------------------------------------------------------------------
50	HHSearch		59.80	42%	-113	- C1 -	4AU8	2	CDK5_HUMAN	A:[11-293]	-----------------------LEKIGEGTYGTVFKAKNRE--THEIVALKRV----------------P----SSALREICLLKELKHKNIVRLHDVLH----------------KLTLVFEFCDQDLKKYFDSC-----N---GDLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINR----NGELKLADFGLARAFGIP-VR-----SAEVVTLWYRPPDVLFGAKLYSTSIDMWSAGCIFAELANA----------GRPLFPGND---V----------DDQLKRIFRLLGTPTEEQWPSMTKLPDY-KPYPM----------YPATTSLV----NVVPKL--NATGRDLLQNLLKCNPVQRISAEEALQHPYFSD--F--------------------------------------------------------------------------------------------------------------------------------------------------------------------
18	PsiBlast_PDB		59.58	40%	-93	- C1 -	3PY1	4	CDK2_HUMAN	A:[17-304]	-------------------------KIGEGTYGVVYKARNKLTGEV--VALKKIRLDTETEG---V----P----STAIREISLLKELNHPNIVKLLDVI--H----T---E----NKLYLVFEFLHQDL---KKFMDASALTGIPLPL----IKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGA----IKLADFGLARAFGVP-VRTYTH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-----------RRALFPGDS---EI----------DQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQ-----------------DFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL---------------------------------------------------------------------------------------------------------------------------------------------------------------
38	HHSearch		58.81	32%	-113	- C1 -	4IC7	1	MK07_HUMAN	A:[60-356]	-----------------------IETIGNGAYGVVSSARRRL--TGQQVAIKKI----PNAF---DVVTNA----KRTLRELKILKHFKHDNIIAIKDILRPTVPYGE---F----KSVYVVLDLMESDLHQIIHS-------S--QPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVN----ENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLA-----------RRQLFPGKN-------------YVHQLQLIMMVLGTPSPAVIQAVGAE------RVRAYIQSLPP--RQP-VPWETVY----PGA--DRQALSLLGRMLRFEPSARISAAAALRHPFLAK--YHDPD----------------------------------------------------------------------------------------------------------------------------------------------------------------
48	HHSearch		53.81	35%	-97	- C1 -	3QYZ	13	MK01_RAT	A:[32-326]	-----------------------LSYIGEGAYGMVCSAYDNL--NKVRVAIKKI----SPFEHQTY----C----QRTLREIKILLRFRHENIIGINDIIRAP----TIEQM----KDVYIVQDLMETDLYKLLKTQ----------HLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNT----TCDLKICDFGLARVADPD-HDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLS-----------NRPIFPGKH-------------YLDQLNHILGILGSPSQEDLNCIINLKA------RNYLLSLPH--KNK-VPWNRLF----PNA--DSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQ--YYDPSDE--------------------------------------------------------------------------------------------------------------------------------------------------------------
47	HHSearch		50.14	35%	-116	- C1 -	2FST	3	MK14_HUMAN	X:[34-323]	-----------------------LSPVG----GSVCAAFDTK--TGLRVAVKKL----SRPF---Q----SIIHAKRTYRELRLLKHMKHENVIGLLDVFTPA----R---SLEEFNDVYLVTHLMGADLN-----Q----------KLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNE----DCELKILD----------------------ATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLT-----------GRTLFPGTD-------------HIDQLKLILRLVGTPGAELLKKISS------ESARNYIQS--LTQMPK-MNFANVF----IGA--NPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQ--YHDPDD---------------------------------------------------------------------------------------------------------------------------------------------------------------