@TOME V2.3
(Nov 2016)
Ref.
-
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Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : PF00069_NEK1_H: (2014-08-18 )
MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLV
Atome Classification :
(21 SA)
.........10........20........30........40.-.......50........60........70.--..-------.....80........90....--...----.100.......110.......120..-......130.......140.......150.......160.......170.....-..180.......190.......200.....-..210.....--..220-.......230.......240.....-----..250.......260.......270.......280.......290.......300.......310.......320.......330.......340.......350.......360.......370.......380.......390.......400.......410.......420.......430.......440.......450.......460.......470.......480.......490.......500.......510.......520.......530.......540.......550.......560.......570.......580.......590.......600.......610.......620.......630.......640.......650.......660.......670.......680.......690.......700.......710.......720.......730.......740.......750.......760.......770.......780.......790.......800.......810.......820.......830.......840.......850.......860.......870.......880.......890.......900.......910.......920.......930.......940.......950.......960.......970.......980.......990.......
(Atome)
(Ident)
(Tito)
(Num)
(Ligand)
(Uniprot)
MEK
Y
V
R
L
Q
KIGEGSFG
K
A
I
L
V
K
STEDGR
Q
Y
V
I
K
EI
N
I
S
RMS
S
-
K
E
R
E
E
S
R
R
E
V
A
V
L
ANMKHP
N
I
V
Q
Y
R
E
S
F
EE--NG-------
S
L
Y
I
V
M
D
YCEG
G
D
L
F
K
R
INAQ--KG
V
----
L
FQE
D
Q
I
L
D
W
F
V
Q
I
C
L
A
L
K
H
VH
D
-
R
K
-
I
L
H
R
DIKSQ
N
I
F
LTKDG
T
V
Q
L
G
D
F
G
I
A
R
V
L
N
S
T
V
E
L
A
R
T
CIGTPYYL
S
P
E
I
CEN-KPYNNK
S
D
I
W
A
L
G
C
V
L
Y
E
L
C
T
L
K
H
A
F
EAG
S
-
M
K
N
L
V
L
K
I
IS--GSFP
P
-
V
S
L
H
YSY
D
L
R
S
L
V
S
Q
L
F
KRNPRDR
P
-----
S
V
N
S
I
L
E
K
G
F
I
A
K
R
I
E
K
F
L
S
P
Q
L
I
A
E
E
F
C
L
K
T
F
S
K
FGSQPIPAKRPASGQN
S
I
S
VM
P
A
Q
K
ITK
P
A
A
KYGIP
L
A
Y
K
KYGDKKLHEKKP
L
Q
K
H
K
Q
A
HQ
T
P
E
KRVN
T
G
E
E
R
R
K
I
S
E
E
A
A
R
K
R
R
L
E
F
I
E
K
E
K
K
Q
K
D
Q
I
I
S
L
M
K
A
E
Q
M
K
R
Q
E
K
E
R
L
E
R
I
N
R
A
R
E
Q
G
W
R
N
V
L
SAGGSGEVKA
P
F
LGSGGTIAPSSFSSRGQ
Y
E
H
Y
H
A
I
F
D
Q
M
Q
Q
Q
RAED
N
E
A
K
W
K
R
E
I
YGRGLPERGILPGVRPGFPYGAAGHHHFP
D
A
D
D
I
R
K
T
L
K
R
L
K
A
V
S
K
QANANRQK
G
Q
L
A
V
E
R
A
K
Q
V
E
E
F
L
Q
R
K
R
E
A
M
Q
N
K
ARAEG
H
M
V
Y
L
A
R
L
R
Q
I
R
L
QNF
N
E
R
Q
Q
I
K
A
K
LRGEKKEANHSEGQEG
S
E
E
A
D
M
R
R
K
K
I
E
S
L
K
A
HA
N
A
R
A
A
V
L
K
E
Q
L
E
R
K
R
K
E
A
Y
E
R
E
K
K
V
W
E
E
H
L
V
AKGVKSSDVSPPLGQHETGGS
P
S
K
Q
Q
M
R
S
V
I
S
V
T
S
A
L
K
EVGVDSSLTD
T
R
E
T
S
E
E
M
Q
K
TNNA
I
S
S
K
R
E
I
L
R
R
L
N
E
NLKAQEDEKGKQNLSD
T
F
E
I
N
VHE
D
A
K
E
H
E
K
E
KSVSS
D
R
K
K
WEAG
G
Q
L
V
I
P
L
D
E
L
T
LDTSFSTTERHTVG
E
V
I
K
LGPNGSPRRAWGKSP
T
D
S
V
L
K
I
L
G
E
A
E
L
Q
L
Q
T
E
L
LENTTIRSEISPEGEKYKPLITGE
K
K
V
Q
C
I
SHEI
N
P
S
A
IVDSPVETKSPEFSEASP
Q
M
S
L
K
LEGNLEEPD
D
L
E
T
E
I
LQEPSGTNKDESL
P
C
T
I
T
D
V
WISEEKETKETQSA
D
R
I
T
I
QENEVSED
G
V
S
S
T
V
D
Q
L
S
D
I
H
IEPGTNDSQHSKCDVDKSVQP
E
P
F
F
H
K
V
V
H
S
EHLNLV
21
PsiBlast_CBE
93.81
99%
-111
- C1 -
4B9D
2
NEK1_HUMAN
B:
[23-306]
M
E
K
Y
V
R
L
Q
K
I
G
-----
K
A
I
L
V
K
S
T
E
D
G
R
Q
Y
V
I
K
E
I
N
I
S
R
M
S
S
-
K
E
R
E
E
S
R
R
E
V
A
V
L
A
N
M
K
H
P
N
I
V
Q
Y
R
E
S
F
E
E
--
N
G
-------
S
L
Y
I
V
M
D
Y
C
E
G
G
D
L
F
K
R
I
N
A
Q
--
K
G
V
----
L
F
Q
E
D
Q
I
L
D
W
F
V
Q
I
C
L
A
L
K
H
V
H
D
-
R
K
-
I
L
H
R
D
I
K
S
Q
N
I
F
L
T
K
D
G
T
V
Q
L
G
D
F
G
I
A
R
V
L
N
S
T
V
E
L
A
R
A
C
I
G
T
P
Y
Y
L
S
P
E
I
C
E
N
-
K
P
Y
N
N
K
S
D
I
W
A
L
G
C
V
L
Y
E
L
C
T
L
K
H
A
F
E
A
G
S
-
M
K
N
L
V
L
K
I
I
S
--
G
S
F
P
P
-
V
S
L
H
Y
S
Y
D
L
R
S
L
V
S
Q
L
F
K
R
N
P
R
D
R
P
-----
S
V
N
S
I
L
E
K
G
F
I
A
K
R
I
E
K
F
L
S
P
Q
L
I
A
E
E
F
C
L
K
T
F
S
K
F
G
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
32
HHSearch
92.67
100%
-112
- C1 -
4B9D
1
NEK1_HUMAN
A:
[23-306]
M
E
K
Y
V
R
L
Q
K
I
------
K
A
I
L
V
K
S
T
E
D
G
R
Q
Y
V
I
K
E
I
N
I
S
R
M
S
S
-
K
E
R
E
E
S
R
R
E
V
A
V
L
A
N
M
K
H
P
N
I
V
Q
Y
R
E
S
F
E
E
--
N
G
-------
S
L
Y
I
V
M
D
Y
C
E
G
G
D
L
F
K
R
I
N
A
Q
--
K
G
V
----
L
F
Q
E
D
Q
I
L
D
W
F
V
Q
I
C
L
A
L
K
H
V
H
D
-
R
K
-
I
L
H
R
D
I
K
S
Q
N
I
F
L
T
K
D
G
T
V
Q
L
G
D
F
G
I
A
R
V
L
N
S
T
V
E
L
A
R
A
C
I
G
T
P
Y
Y
L
S
P
E
I
C
E
N
-
K
P
Y
N
N
K
S
D
I
W
A
L
G
C
V
L
Y
E
L
C
T
L
K
H
A
F
E
A
G
S
-
M
K
N
L
V
L
K
I
I
S
--
G
S
F
P
P
-
V
S
L
H
Y
S
Y
D
L
R
S
L
V
S
Q
L
F
K
R
N
P
R
D
R
P
-----
S
V
N
S
I
L
E
K
G
F
I
A
K
R
I
E
K
F
L
S
P
Q
L
I
A
E
E
F
C
L
K
T
F
S
K
F
G
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
33
HHSearch
61.00
43%
-95
- C1 -
2W5A
5
NEK2_HUMAN
A:
[5-272]
A
E
D
Y
E
V
L
Y
T
I
G
T
G
S
Y
G
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
K
G
T
K
E
R
Q
----
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
--
L
H
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
G
L
A
R
I
L
---
T
S
F
A
K
T
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
-
M
-
Y
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
L
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
18
PsiBlast_PDB
60.47
42%
-102
- C1 -
2JAV
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
G
S
Y
G
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
-----------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
RQ
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
D-----
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
G
L
A
R
I
L
-----------
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
-
M
S
Y
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
11
PsiBlast_PDB
58.02
42%
-99
- C1 -
2XKD
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
G
---
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
RQ
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
D-----
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
--------------
T
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
-
M
S
Y
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
12
PsiBlast_PDB
57.65
42%
-97
- C1 -
2XKF
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
G
---
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
RQ
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
----
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
---------------
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
--
S
Y
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
19
PsiBlast_PDB
57.59
42%
-94
- C1 -
2W5B
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
G
S
Y
G
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
RQ
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
--
L
H
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
G
L
A
R
I
L
N
H
D
T
S
F
A
K
A
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
-
M
S
Y
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
14
PsiBlast_PDB
57.54
42%
-96
- C1 -
2XNM
10
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
G
S
Y
G
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
R
Q
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
-----
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
G
L
A
R
I
L
---------
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
--
S
Y
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
16
PsiBlast_PDB
56.35
42%
-93
- C1 -
2XNO
13
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
G
S
Y
G
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
R
Q
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
----
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
---------------
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
----
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
7
PsiBlast_PDB
55.90
42%
-94
- C1 -
2XK6
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
-----
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
RQ
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
----
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
-----------------
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
----
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
13
PsiBlast_PDB
55.71
42%
-102
- C1 -
2XKE
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
G
---
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
RQ
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
D-----
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
------------------
T
P
Y
Y
M
S
P
E
Q
M
N
-----
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
6
PsiBlast_PDB
53.73
42%
-94
- C1 -
2XK4
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
--
Y
G
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
R
Q
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
----
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
G
L
-------------
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
-
M
--
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
17
PsiBlast_PDB
53.31
42%
-91
- C1 -
2XNP
6
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
G
-
Y
G
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
R
Q
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
---
H
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
G
L
-------------
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
----
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
4
PsiBlast_PDB
53.28
42%
-97
- C1 -
2WQO
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
G
---
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
RQ
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
-------
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
------------------
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
----
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
8
PsiBlast_PDB
52.56
42%
-94
- C1 -
2XK7
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
G
---
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
R
Q
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
----
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
----------------
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
-----
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
5
PsiBlast_PDB
52.49
42%
-93
- C1 -
2XK3
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
----
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
R
Q
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
----
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
G
---------------
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
----
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
15
PsiBlast_PDB
50.96
42%
-90
- C1 -
2XNN
6
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
--
Y
G
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
R
Q
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
----
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
G
--------------
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
---
Y
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
9
PsiBlast_PDB
50.93
42%
-96
- C1 -
2XK8
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
----
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
RQ
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
----
R
D
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
F
---------------
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
-----
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
10
PsiBlast_PDB
48.51
42%
-91
- C1 -
2XKC
1
NEK2_HUMAN
A:
[6-271]
-
E
D
Y
E
V
L
Y
T
I
G
T
--
Y
G
R
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
K
E
L
D
Y
G
S
M
T
E
-
A
E
K
Q
M
L
V
S
E
V
N
L
L
R
E
L
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
RT
N
T
-------
T
L
Y
I
V
M
E
Y
C
E
G
G
D
L
A
S
V
I
T
----
K
G
T
K
E
RQ
Y
L
D
E
E
F
V
L
R
V
M
T
Q
L
T
L
A
L
K
E
C
H
R
-
R
S
------
L
K
P
A
N
V
F
L
D
G
K
Q
N
V
K
L
G
D
------------------
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
----
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
L
C
A
L
M
P
P
F
T
A
F
S
-
Q
K
E
L
A
G
K
I
R
E
--
G
K
F
R
R
-
I
P
Y
R
Y
S
D
E
L
N
E
I
I
T
R
M
L
N
L
K
D
Y
H
R
P
-----
S
V
E
E
I
L
E
N
P
L
I
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
39
HHSearch
48.38
31%
-85
- C1 -
4GV1
5
AKT1_HUMAN
A:
[7-269]
M
N
E
F
E
Y
L
K
L
L
G
K
G
T
F
G
K
V
I
L
V
K
E
K
A
T
G
R
Y
Y
A
M
K
I
L
K
K
E
V
I
V
A
K
D
E
V
A
H
T
L
T
E
N
R
V
L
Q
N
S
R
H
P
F
L
T
A
L
K
Y
S
F
Q
T
--
H
D
-------
R
L
C
F
V
M
E
Y
A
N
G
G
E
L
F
F
H
L
S
R
E
--
R
------
V
F
S
E
D
R
A
R
F
Y
G
A
E
I
V
S
A
L
D
Y
L
H
S
E
K
N
-
V
V
Y
R
D
L
K
L
E
N
L
M
L
D
K
D
G
H
I
K
I
T
D
F
G
L
C
K
E
G
I
K
D
G
A
T
M
K
T
F
C
G
T
P
E
Y
L
A
P
E
V
L
E
D
-
N
D
Y
G
R
A
V
D
W
W
G
L
G
V
V
M
Y
E
M
M
C
G
R
L
P
F
Y
N
Q
D
-
H
E
K
L
F
E
L
I
L
M
--
E
E
I
-
R
-
F
P
R
T
L
G
P
E
A
K
S
L
L
S
G
L
L
K
K
D
P
K
Q
R
L
GGGS
E
D
A
K
E
I
M
Q
H
R
F
F
A
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
43
HHSearch
38.42
30%
-86
- C1 -
1ZY4
1
GCN2_YEAST
A:
[6-288]
-
S
D
F
E
E
I
A
V
L
G
Q
G
A
F
G
Q
V
V
K
A
R
N
A
L
D
S
R
Y
Y
A
I
K
K
I
R
H
---
T
E
-
E
K
L
S
T
I
L
S
E
V
M
L
L
A
S
L
N
H
Q
Y
V
V
R
Y
Y
A
A
W
L
E
--
R
R
N
F
VK
K
K
S
T
L
F
I
Q
M
E
Y
C
E
N
G
T
L
Y
D
L
I
H
S
---
E
N
L
----
N
Q
Q
R
D
E
Y
W
R
L
F
R
Q
I
L
E
A
L
S
Y
I
H
S
-
Q
G
-
I
I
H
R
D
L
K
P
M
N
I
F
I
D
E
S
R
N
V
K
I
G
D
F
G
L
A
K
N
V
H
R
-----------
A
M
Y
V
A
T
E
V
L
D
G
T
G
H
Y
N
E
K
I
D
M
Y
S
L
G
I
I
F
F
E
M
I
Y
---
P
F
S
T
G
M
E
R
V
N
I
L
K
K
L
R
S
VS
I
E
F
P
P
D
F
D
D
N
K
M
K
V
E
K
K
I
I
R
L
L
I
D
H
D
P
N
K
R
P
-----
G
A
R
T
L
L
N
S
G
W
L
P
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------