@TOME V2.3
(Nov 2016)
Ref.
-
-
Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : Lmo0290: (2016-03-14 )
MDWRKTQMIFIVTLLILNVFLAVIFFNKQLSDDPDTLGNETLEERLKADNISHPDLSTKPTSGSIFTTERAAFTTKDVSDLTGQAITLKDNNKQIYSVLQTPVKAGKKGSNPEFQKFMESDVYRGESYQFWNYDKDARTLTFNQLINNNMVLFDEAGQITFYLDQDDRVISYEQTWMSKQDDLKDKTNLMSATDALEAVYQHGELKQDSDVVSATFGYYTTVQLPSGNVYFPVWCFEVKHSGETNYVLVNAKDEQVINQSENKSTTEPGAELSSRQKAK
Atome Classification :
(20 SA)
-----------------------------------------------------.....-....10---------..-----------.....-----..20.-----.......30-----..--------.......----40.....-...50.....-------...----.60-------......-..70....-.....80......-.-.-----90.-----......------.100.....--------------------..110.......120----.......-----130..---.....140-......-.---150------.------...------...160.......170.-.......180------------.--....-...190.-.....-.200---------------------..----.....210....--------...220......-----------.230.......240--.---..-----..-------....250.......260-.........270.......---------------------------------
(Atome)
(Ident)
(Tito)
(Num)
(Ligand)
(Uniprot)
-----------------------------------------------------MDW
R
K
-
T
Q
M
I
F
----------
I
V
-----------
T
L
L
I
L
-----
N
V
F
L
A
-----
V
I
F
FNKQL------SD--------DPDTL
G
N
----
E
T
L
E
E
R
L
-KADN-ISHPD-------LST----KPT-------SG
S
I
F
T
-TER-AAFT-TK
D
V
S
DLTG
Q
A
I
T
-L-K-----DN
N
-----
K
Q
I
Y
S
V
------
L
Q
T
P
V
KAGK--------------------KGS
N
P
E
F
Q
K
F
M
E
S
DV----YRGE
S
Y
Q
-----
F
WNYD---KDA
R
T
L
T
F
-
N
Q
LINN-
N
---
M
V
L
------
F
------DEA------G
Q
I
T
F
Y
LDQDD
R
V
I
S
-
Y
-
E
Q
T
WMSKQD-------------D--L
K
D
K
-
T
N
L
M
S
A
T
-
D
A
L
E
A
-
V
Y
QH---------------------GE----LKQDSD
V
V
S
A
T
F--------G
Y
Y
T
T
V
Q
LPSG
N
-----------
V
Y
F
P
V
W
C
F
E
V
KH----S---GE-----T
N
-------
Y
V
L
V
NAK
D
E
Q
V
I
NQS---ENKSTTEPGAELSSRQKAK---------------------------------
6
HHSearch
97.32
26%
-69
- C5 -
2O3O
1
YYCI_BACSU
A:
[14-252]
-----------------------------------------------------------------------------------------------------------------------------------------
K
----
N
D
V
E
H
D
M
-
K
A
D
H
-
I
T
Y
E
G
-------
L
N
K
----
E
A
T
-------
E
G
Y
R
I
T
-
A
N
Q
-
K
S
F
S
-
K
E
E
I
E
A
L
K
D
Q
K
P
L
-
M
-
D
MPSDD
H
K
V
-----
T
S
L
K
M
K
------
F
A
N
P
I
A
L
S
K
--------------------
K
D
I
E
D
D
A
Q
A
L
V
S
S
K
I
----
Q
D
G
E
K
Y
K
-----
L
W
K
V
D
---
K
S
K
K
E
I
I
F
-
F
Q
T
Y
E
G
-
H
---
Y
I
Y
------
Q
KTDNPS
N
M
I
------
G
Q
V
V
L
H
L
N
G
K
N
E
V
V
S
-
Y
-
D
Q
T
T
L
E
T
F
K
-------------
Q
--
I
-
Q
K
-
E
S
L
I
T
E
M
-
D
A
V
E
L
-
L
Y
Y
Q
---------------------
N
Q
----
L
K
E
Y
S
T
V
K
S
C
K
F
--------
G
Y
V
A
Q
Y
P
L
T
S
T
Q
-----------
V
L
A
P
V
W
R
I
T
V
E
Y
--------
E
K
EKKT
V
Q
E
-------
Y
F
T
V
N
A
L
E
S
T
I
L
D
T
--------------------------------------------------------
1
PsiBlast_PDB
94.56
26%
-74
- C5 -
2O3O
1
YYCI_BACSU
A:
[17-251]
------------------------------------------------------------------------------------------------------------------------------------------------
V
E
H
D
M
-
K
A
D
H
-
I
T
Y
E
G
-------
L
N
K
----
E
A
T
-------
E
G
Y
R
I
T
-
A
N
Q
-
K
S
F
S
-
K
E
E
I
E
A
L
K
D
Q
K
-
P
-
L
-
M
-----
D
M
P
-----
S
D
D
H
K
V
T
S
L
K
M
K
F
A
N
P
I
A
L
S
K
--------------------
K
D
I
E
D
D
A
Q
A
L
V
S
S
K
I
----
Q
D
G
E
K
Y
K
-----
L
W
K
V
D
---
K
S
K
K
E
I
I
F
-
F
Q
T
Y
E
G
-
H
---
Y
I
Y
QKTDNP
S
------
N
M
I
------
G
Q
V
V
L
H
L
N
G
K
N
E
V
V
S
-
Y
-
D
Q
T
T
L
E
T
F
K
-------------
Q
--
I
Q
-
K
-
E
S
L
I
T
E
M
-
D
A
V
E
L
-
L
Y
Y
Q
---------------------
N
Q
----
L
K
E
Y
S
T
V
K
S
C
K
F
--------
G
Y
V
A
Q
Y
P
L
T
S
T
Q
-----------
V
L
A
P
V
W
R
I
T
V
E
Y
----
E
---
K
--------EKKT
V
Q
E
Y
F
T
V
N
A
L
E
S
T
I
L
D
---------------------------------------------------------
16
Fugue
92.95
25%
-57
- C5 -
2O3O
1
YYCI_BACSU
A:
[8-252]
------------------------------------------------------------------------------------------------------------------------------------
K
E
Y
E
V
I
----
K
N
D
V
E
H
D
M
K
A
D
H
-
I
T
Y
E
G
-------
L
N
K
----
E
A
T
-------
E
G
Y
R
I
T
-
A
N
Q
-
K
S
F
S
-
K
E
E
I
E
A
L
K
D
Q
K
P
L
M
D
M
P
-----
S
D
D
-----
H
K
V
T
S
L
K
M
K
---
F
A
N
P
I
A
L
S
K
--------------------
K
D
I
E
D
D
A
Q
A
L
V
S
S
K
I
----
Q
D
G
E
K
Y
K
-----
L
W
K
V
D
---
K
S
K
K
E
I
I
F
-
F
Q
T
Y
E
G
-
H
---
Y
I
Y
------
Q
------
K
T
D
NPSNMI
G
Q
V
V
L
H
L
N
G
K
N
E
V
V
S
-
Y
-
D
Q
T
T
L
E
T
F
K
-------------
Q
--
I
Q
K
E
-
S
L
--
I
T
E
M
D
A
V
E
L
-
L
Y
Y
Q
---------------------
N
Q
----
L
K
E
Y
S
T
V
K
S
C
K
F
--------
G
Y
V
A
Q
Y
P
L
T
S
T
Q
-----------
V
L
A
P
V
W
R
I
T
V
E
Y
E
KEK
K
---
T
V
-----
Q
E
-------
Y
F
T
V
N
A
L
E
S
T
I
L
D
T
--------------------------------------------------------
17
Fugue
70.62
19%
-9
* C6 *
3FHC
-
NU214_HUMAN
A:
[9-405]
---------------------------------------------I
P
E
R
E
M
KD
F
Q
F
R
A
-
L
K
K
V
R
----------
I
F
DSPEELPKER
S
S
L
L
A
V
SNKYG
L
V
F
A
G
G
AS
G
L
Q
I
F
P
T
K
N
L
L
I
Q
N
K
P
G
D
--------
D
P
N
K
I
V
D
----
K
V
Q
G
L
L
V
-
P
M
K
F
P
I
H
H
L
A
-------
L
S
C
DN--
L
T
L
-------
S
A
C
M
M
S
-
S
E
Y
G
S
I
I
A
-
F
F
D
V
R
T
F
S
N
E
A
Q
K
-
R
-
P
-----
F
A
Y
-----
H
K
L
L
K
D
------
A
G
G
M
V
I
D
M
K
W
-------------------
N
P
T
V
P
S
M
V
A
V
C
L
A
D
--------
G
S
I
A
-----
V
L
Q
V
T
---
E
T
V
K
V
C
A
T
L
P
S
T
V
A
V
-
T
---
S
V
C
------
W
------
S
P
K
------
G
K
Q
L
A
V
G
K
Q
N
G
T
V
V
Q
-
Y
-
L
P
T
L
Q
E
K
K
V
I
P
CPPFYESD
H
P
V
R
--
V
L
D
V
-
L
W
I
G
T
Y
V
-
F
A
I
V
Y
-
A
A
A
D
---------------------
G
T
----
L
E
T
S
P
D
V
V
M
A
L
L
P
KKEEK
H
P
E
I
F
V
N
F
M
E
P
C
Y
G
SCTERQ
H
H
Y
Y
L
S
Y
I
E
E
W
D
L
V
L
A
A
----
S
---
A
A
-----
S
T
-------
E
V
S
I
L
A
R
Q
S
D
Q
I
N
W
E
---
S
W
L
L
E
D
S
S
R
A
E
L
P
V
T
D
K
S
D
D
S
L
P
M
G
V
V
V
D
YTNA
P
V
L
M
L
L
S
TDG
V
L
C
P
F
Y
M
I
N
8
HHSearch
57.36
10%
-57
- C5 -
3NQZ
-
?
A:
[38-176]
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
N
E
L
V
-----
V
L
K
E
F
TSN
N
G
E
V
T
R
R
Y
-
Q
Q
T
Y
Q
G
-
I
---
P
-
V
------
I
------
G
D
T
--------
V
S
L
T
F
N
-
N
G
M
L
K
K
-
A
-
H
G
A
A
V
Y
N
I
D
-------------
E
D
-
L
S
D
V
-
S
A
K
L
T
K
K
-
D
A
I
L
K
-
G
S
K
T
---------------------
G
I
A
A
K
S
V
G
L
K
K
H
N
E
Q
S
R
L
--------
A
I
W
V
D
----
D
Q
N
-----------
K
A
H
L
V
Y
E
V
S
Y
V
T
----
Y
G
K
S
P
S
-----
R
P
-------
Y
L
I
I
D
A
N
T
G
E
V
L
L
S
Y
---
D
---------------------------------------------------
18
Fugue
55.32
17%
5
- C7 -
4RUW
3
?
A:
[53-438]
----------------------------------------------------R
R
D
L
R
V
M
T
W
N
I
H
TAVAPDAPGV
V
D
-----------
E
P
R
I
A
-----
A
V
I
R
A
E
APD-
V
V
V
L
N
E
V
H
------
R
D
--------
A
P
G
P
G
S
H
GD
Q
P
A
R
L
A
E
L
L
-
A
A
D
G
-
Y
V
H
T
W
-------
F
G
L
TEVD
L
P
H
-------
E
G
A
V
L
P
G
S
S
N
-
G
N
V
V
M
S
R
H
P
F
V
G
G
G
V
V
V
P
-
L
-
P
-----
N
E
N
YEPG
G
K
L
R
R
S
L
------
L
T
V
T
V
D
V
P
G
L
G
D
V
V
V
H
A
T
H
L
S
T
PGS
A
V
L
V
E
D
Q
K
E
Q
L
R
I
V
L
D
H
V
D
----
A
R
V
P
S
V
L
A
G
DLN
I
W
T
T
D
---
V
P
T
Q
P
Y
S
Q
-
N
---
N
L
M
Q
---
S
W
I
------
A
------
E
D
H
------
L
A
D
T
W
R
Q
V
N
D
P
G
A
G
P
-
T
M
T
A
S
Y
G
R
P
E
S
-------------
P
--
H
P
D
R
R
I
D
Y
V
F
A
T
-
P
A
F
D
V
-
V
A
G
H
VSLVDRFASD
H
L
G
V
V
M
D
L
R
L
G
G
A
----
P
V
A
A
R
T
V
L
A
G
E
D
--------
G
L
D
G
W
A
Q
L
T
A
S
R
-----------
P
G
-
R
L
R
L
S
V
C
K
N
----
R
---
G
Q
-----
A
D
DDG
T
A
V
-
R
A
V
L
R
N
R
A
G
V
A
L
R
T
V
---
T
D
S
G
T
S
R
D
R
C
T
V
E
T
W
R
G
A
L
P
PG
A
R
L
E
A
C
L
V
G
ADGTIL
A
S
R
T
E
T
L--------
9
HHSearch
48.82
11%
-64
- C5 -
2GU3
-
YPMB_BACSU
A:
[70-133]
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
E
A
-
K
E
G
I
S
E
D
-
K
A
A
K
I
-
I
K
D
E
---------------------
G
-------
L
V
S
K
Q
K
E
V
H
L
--------
A
R
E
G
--------------------
N
V
L
L
W
E
V
T
Y
L
D
----
K
E--
G
Q
-----
Y
S
-------
L
S
Y
V
D
F
T
T
G
K
I
L
K
N
--------------------------------------------------------
25
Fugue
46.71
16%
-27
- C4 -
1DRM
1
A:
[13-131]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
I
P
D
A
M
I
V
------
I
------
D
G
H
------
G
I
I
Q
L
F
S
T
A
A
E
R
L
F
G
-
W
-
S
E
L
E
A
I
G
Q
N
------------------
V
N
--
I
L
M
P
E
P
-
D
R
S
R
H
-
D
S
Y
I
---------------------
S
R
----
Y
R
T
T
S
D
P
H
I
I
G
I
--------
G
R
I
V
T
G
K
R
R
D
G
T
-----------
T
F
P
M
H
L
S
I
G
E
M
Q
----
S
---
G
G
-----
E
P
-------
Y
F
T
G
F
V
R
D
L
T
E
H
Q
Q
T
---
Q
A
R
L
Q
E
L
Q
--------------------------------------------
22
Fugue
44.08
13%
6
- C7 -
4FFL
8
?
A:
[1-363]
M
K
T
I
C
L
VGGK
L
Q
G
F
E
A
A
Y
L
S
K
K
A
G
M
K
V
V
L
V
D
KNPQALIRNYAD
E
F
Y
C
F
D
VIKE
P
E
K
L
L
-
E
L
S
K
R
----------
V
D
-----------
A
V
L
P
V
-----
N
E
N
L
A
-----
C
I
E
F
L
N
S
I
------
K
E
KFSCPVLF
D
F
E
A
Y
R
I
S
R
D
K
K
K
S
K
D
Y
F
-
K
S
I
G
-
V
P
T
P
Q
DRPSKPP
Y
F
V
----
K
P
P
CESSSVG
A
R
I
I
Y
G
-
L
E
P
-
D
T
L
V
-
E
E
Y
V
E
G
E
V
V
S
L
E
V
-
V
-
G
-----
D
G
S
-----
H
F
A
V
-
V
------
K
E
T
L
V
H
I
D
E
--------------------
T
Y
D
C
H
M
V
T
P
L
P
A
N
P
L
----
F
R
Q
I
S
H
D
-----
L
A
A
N
L
---
P
L
K
G
I
M
D
V
-
E
A
I
F
G
P
-
K
---
G
L
R
------
V
------
I
E
I
DARFP-
S
Q
T
P
T
V
V
Y
Y
S
S
G
I
N
L
-
I
-
E
L
L
F
R
A
F
T
D
-------------
G
--
V
E
E
I
-
E
N
K
Y
C
I
Y
-
E
H
L
M
F
-
G
-
E
N
---------------------
G
V
----
L
I
P
V
G
E
Q
V
L
S
M
G
--------
S
D
Y
G
K
F
Y
E
E
P
G
----------------
I
E
I
F
L
C
K
G
----
E
---
Y
P
-----
V
F
-------
T
M
V
F
W
G
K
D
R
E
E
T
G
A
K
---
R
C
K
G
L
S
V
L
K
E
R
F
G
A
V
L
------------------------------------
3
PsiBlast_PDB
42.36
22%
-8
* C8 *
4LR4
5
?
A:
[121-226]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
D
E
----
N
Q
G
H
Y
Y
Q
-----
C
V
V
S
D
---
E
D
C
N
N
I
T
F
-
M
L
E
S
N
V
-
N
---
V
F
I
------
F
------
D
N
D
------
I
N
L
I
Y
R
S
S
D
E
A
G
V
T
S
-
Y
-
F
D
R
Y
Y
S
T
T
K
-------------
T
--
I
A
G
T
-
S
N
K
V
I
S
L
-
G
L
I
D
G
N
Y
Y
I
V
---------------------
F
K
----
V
K
D
A
T
A
T
T
G
Y
H
Y
--------
G
Y
Y
A
G
Q
P
L
P
------------------------------------------------------------------------------------------------------------------------
10
HHSearch
36.86
16%
-1
- C5 -
2KGY
-
?
A:
[32-95]
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
D
E
-
V
T
G
P
D
A
D
-
R
A
R
A
A
-
A
V
Q
A
---------------------
V
-------
P
G
G
T
A
G
E
V
E
T
--------
E
T
G
-------
E
G
-------------
A
A
A
Y
G
V
L
V
T
R
----
P
D--
G
T
-----
R
V
-------
E
V
H
L
D
R
-
D
F
R
V
L
D
T
E
-------------------------------------------------------
23
Fugue
34.88
21%
-69
* C7 *
4TNJ
150
PSBT_THEEB
T:
[1-32]
-------------------------------------------------------------
M
E
T
----------
I
T
-----------
Y
V
F
I
F
-----
A
C
I
I
A
-----
L
F
F
F
A
I
F
F
------
R
E
--------
P
P
R
I
T
K
K
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2
PsiBlast_PDB
33.83
32%
58
- C3 -
3P0S
-
?
A:
[149-204]
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
T
T
S
S
V
A
N
A
N
-----------
A
Q
Y
P
I
G
C
F
H
F
D
E
----
A
---
Y
E
-----
T
N
-------
Y
G
I
N
N
V
A
D
--
I
I
N
K
A
L
GT
E
W
K
N
A
T
R
P
T
A
A
V
T
T
--------------------------------------
21
Fugue
31.93
17%
12
- C7 -
2KCL
-
?
A:
[1-99]
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
E
D
P
E
D
P
F
T
R
Y
A
L
A
Q
E
----
H
L
K
H
D
-----------
N
---
A
S
R
A
L
A
L
F
-
E
E
L
V
E
T
-
D
PDY
V
G
T
------
Y
------
Y
H
L
------
G
K
L
Y
E
R
L
D
R
T
D
D
A
I
D
T
Y
-
A
Q
G
I
E
V
A
R
E
-------------
E
GT
Q
K
D
L
-
S
E
L
Q
D
A
K
-
L
K
A
E
G
-
L
E
H
H
---------------------
H
H
----
H
H
---------------------------------------------------------------------------------------------------------------------------------------------------
15
HHSearch
31.78
8%
52
- C5 -
2JXW
-
A:
[3-27]
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
S
K
G
R
W
V
E
G
I
T
S
----
E
---
G
-------
Y
-------
H
Y
Y
Y
D
L
I
S
G
A
S
-----------------------------------------------------------
5
PsiBlast_PDB
29.51
19%
29
- C5 -
4PKX
-
?
A:
[25-168]
-------------------------------------------------------------------------------------------------------------------------------------------------------------
Y
P
P
-------
L
Y
E
----
R
E
A
-------
Q
----
D
-
N
A
E
-
L
G
M
I
-
K
P
D
Q
L
A
S
L
N
Q
A
L
T
-
D
-
S
-----
Y
T
T
-----
R
D
T
N
S
I
------
L
R
S
--------------------------
R
M
S
V
S
Y
L
K
G
Y
T
Y
E
D
I
LIVR
F
R
P
E
S
E
I
-----
Y
W
P
I
S
---
Q
D
S
R
N
A
M
I
-
D
K
L
S
-
N
-
T
---
S
V
N
------
F
------
E
V
S
------
L
E
F
T
R
P
-----
A
A
L
K
-
H
-
S
K
S
W
L
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
4
PsiBlast_PDB
26.46
34%
125
- C5 -
4NS4
-
?
A:
[29-75]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
N
T
-
T
Q
K
-
I
I
Q
Y
-
E
R
S
K
S
D
L
T
T
Q
S
F
T
-
L
-
S
-----
S
G
D
-----
K
I
V
Y
A
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
20
Fugue
26.17
31%
148
- C6 -
2B5K
1
PPM1_LIMPO
A:
[1-19]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
R
R
W
C
F
R
V
C
Y
----
R
---
G
R
-----
F
C
-------
Y
R
K
C
R
-----------------------------------------------------------------
11
HHSearch
25.91
24%
99
- C8 -
1E0N
-
A:
[7-27]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
P
G
W
E
I
I
H
E
N
--------
G
-------
R
-------
P
L
Y
Y
N
A
E
Q
K
T
------------------------------------------------------------
13
HHSearch
23.08
50%
379
- C2 -
2D10
-
NHRF1_HUMAN
E:
[16-25]
-----------------------------------------------------
M
D
W
S
K
-
K
N
E
L
F
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------