@TOME V2.3
(Nov 2016)
Ref.
-
-
Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : gbs2054: (2015-12-12 )
MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAAQILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVVKREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFESVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEISAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSLYWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVYDIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELESLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGTLKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQSSNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENYHYVRPKFNDQHQIKIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQMGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFDELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLERDGEVTFLHKIESGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSPSQEAVTADLNEELDSEKQQGQLSLFEEPSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKKLL
Atome Classification :
(26 SA)
.........10........20........30........40........50........60........70........80........90........100.......110....-...120..-.....130.......140.......150.......160.......170.......180....-.---.-------------.190.....---..200-----...------.....210.....-..220.......230.......240.......250.......260.......270.......280.......290.......300.......310.......320.......330.......340.......350.......360.......370........380.......390.......400.......410.......420.......430.......440.......450.......460.......470.......480.......490.......500.......510.......520.......530.......540.......550.......560.......570.......580.......590.......600.......610.......620.......630.......640.......650.......660.......670.......680.......690.......700.......710.......720.......730.......740.......750.......760.......770.......780.......790.......800.......810.......820.......830.......840.......850......
(Atome)
(Ident)
(Tito)
(Num)
(Ligand)
(Uniprot)
MAKPTI
S
P
G
M
Q
Q
Y
L
D
I
K
ENYP
D
A
F
L
L
FRM
G
D
F
Y
E
L
F
Y
D
DAVK
A
A
Q
I
L
E
I
S
L
TSRNKNAEKPIPMAGVPY
H
S
A
Q
Q
Y
I
D
V
L
V
ELG
Y
K
V
A
I
A
E
Q
ME
D
P
K
K
A
V
G
V
V
K
R
E
V
V
QVVTPG
T
V
V-ESTKPDSA-N
N
F
L
V
A
IDSQDQ
Q
T
F
G
L
A
Y
M
DVSTGEF
Q
A
T
L
L
T
D
F
E
S
V
R
S
E
I
L
NLK
A
R
E
I
V
V
G
Y
Q
LTDEKN
H
-
L
---
L
-------------
T
K
Q
M
N
L
L
L
S---YEDE------RLN------
D
I
H
L
I
D
E
QLTD
L
E
-
I
S
A
A
E
K
L
L
Q
Y
V
H
R
T
Q
K
R
E
L
S
H
L
Q
K
V
V
H
Y
E
I
K
D
Y
L
Q
M
S
YATKN
S
L
D
L
L
E
NARTSKKHG
S
L
Y
W
L
LDET
K
T
A
M
G
T
R
M
L
R
T
W
IDRP
L
V
S
M
N
R
I
K
E
R
Q
D
I
I
Q
V
F
L
D
Y
F
F
ER
N
D
L
T
E
S
L
K
G
V
Y
D
I
E
R
L
A
S
R
VSFGKA
N
P
K
D
L
L
Q
L
G
Q
T
L
S
QI
P
R
I
K
M
I
L
-
Q
SFNQP
E
L
D
I
I
V
N
KIDT
M
P
E
L
E
S
L
I
N
T
AIAPEA
Q
A
TITEG
N
I
I
K
S
G
F
D
K
Q
L
D
N
Y
R
T
V
M
R
EGTG
W
I
A
D
I
E
A
K
E
R
AASG
I
G
T
L
K
I
D
YNKKD
G
Y
Y
F
H
VTNSNLSL
V
P
E
H
F
F
R
K
ATLKNSERYGT
A
E
L
A
K
I
E
G
E
M
L
E
A
R
EQSSNLE
Y
D
I
F
M
R
V
R
A
Q
V
E
S
Y
I
K
R
L
Q
E
L
A
K
T
I
A
T
V
D
V
L
Q
S
L
A
V
V
A
EN
Y
H
Y
VRPKFNDQ
H
Q
I
K
IKNG
R
H
A
T
V
E
K
V
M
G
V
Q
E
YIPN
S
I
Y
FDSQT
D
I
Q
L
I
TGPNMS
G
K
S
T
Y
M
R
Q
L
A
L
T
V
I
M
A
Q
MGGFVSADEVD
L
P
V
F
D
A
I
F
T
RIGAADDLISGQ
S
T
F
M
V
E
M
M
E
A
N
Q
A
V
K
RASD
K
S
L
I
L
FDELGRGTATY
D
G
M
A
L
A
Q
S
I
I
E
Y
I
H
D
R
V
R
A
K
T
M
F
A
T
H
Y
H
E
L
T
D
L
S
E
Q
L
T
R
L
V
N
V
H
V
A
T
LERDG
E
V
T
F
L
H
KIESGPADKSYG
I
H
V
A
K
IAGL
P
I
D
L
L
D
R
A
T
D
I
L
S
Q
L
E
A
D
A
V
Q
L
I
VSPSQ
E
A
V
T
A
D
L
N
E
E
L
D
S
E
K
QQGQ
L
S
L
FEEPSN
A
G
R
V
I
E
E
L
E
A
IDIMNL
T
P
M
Q
A
M
N
A
I
F
D
L
K
K
LL
44
HHSearch
97.30
37%
-86
- C3 -
1WB9
4
MUTS_ECOLI
A:
[7-798]
--
F
D
A
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
T
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
-
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
-
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
G
RRG
L
R
R
R
P
LWEFE
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
---
T
R
D
--------
L
V
------
G
---
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
-
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
30
PsiBlast_CBE
96.26
36%
-84
- C3 -
1WB9
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
T
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
8
PsiBlast_PDB
96.15
36%
-83
- C3 -
3K0S
3
MUTS_ECOLI
A:
[10-797]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
------------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
N
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
7
PsiBlast_PDB
95.97
36%
-83
- C3 -
1WBD
4
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
Q
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
47
HHSearch
95.80
38%
-79
- C3 -
1EWQ
6
MUTS_THEAQ
A:
[9-764]
-
G
P
G
P
L
P
P
L
L
Q
Q
Y
V
E
L
R
D
Q
Y
P
D
Y
L
L
L
F
Q
V
G
D
F
Y
E
C
F
G
E
D
A
E
R
L
A
R
A
L
G
L
V
L
T
H
K
T
S
K
-
D
F
T
T
P
M
A
G
I
P
L
R
A
F
E
A
Y
A
E
R
L
L
K
M
G
F
R
L
A
V
A
D
Q
V
E
P
A
E
E
A
E
G
L
V
R
R
E
V
T
Q
L
L
T
P
G
T
L
L
-
Q
E
S
L
L
P
R
E
-
A
N
Y
L
A
A
I
A
T
G
--
D
G
W
G
L
A
F
L
D
V
S
T
G
E
F
K
G
T
V
L
K
S
K
S
A
L
Y
D
E
L
F
R
H
R
P
A
E
V
L
L
A
P
E
L
L
E
--
N
G
-
A
---
F
-------------
L
D
E
F
R
K
R
F
P
---
V
M
L
S
-------
E
A
------
P
F
E
--
P
E
G
E
G
P
L
A
L
R
R
A
R
G
A
L
L
A
Y
A
Q
R
T
Q
G
G
A
-
L
S
L
Q
P
F
R
F
Y
D
P
G
A
F
M
R
L
P
E
A
T
L
R
A
L
E
V
F
E
P
L
R
---
G
Q
D
T
L
F
S
V
L
D
E
T
R
T
A
P
G
R
R
L
L
Q
S
W
L
R
H
P
L
L
D
R
G
P
L
E
A
R
L
D
R
V
E
G
F
V
R
E
G
A
L
R
E
G
V
R
R
L
L
Y
R
L
A
D
L
E
R
L
A
T
R
L
E
L
G
R
A
S
P
K
D
L
G
A
L
R
R
S
L
Q
I
L
P
E
L
R
A
L
L
-
G
E
E
V
---------
G
L
P
D
L
S
P
L
K
E
E
L
E
A
A
L
V
E
D
P
P
L
K
V
S
E
G
G
L
I
R
E
G
Y
D
P
D
L
D
A
L
R
A
A
H
R
E
G
V
A
Y
F
L
E
L
E
E
R
E
R
E
R
T
G
I
P
T
L
K
V
G
Y
N
A
V
F
G
Y
Y
L
E
V
T
R
P
Y
Y
E
R
V
P
K
E
Y
R
P
V
Q
T
L
K
D
R
Q
R
Y
T
L
P
E
M
K
E
K
E
R
E
V
Y
R
L
E
A
L
I
R
R
R
E
E
E
V
F
L
E
V
R
E
R
A
K
R
Q
A
E
A
L
R
E
A
A
R
I
L
A
E
L
D
V
Y
A
A
L
A
E
V
A
V
R
Y
G
Y
V
R
P
R
F
G
D
--
R
L
Q
I
R
A
G
R
H
P
V
V
E
R
R
---
T
E
F
V
P
N
D
L
E
M
A
--
H
E
L
V
L
I
T
G
P
N
M
A
G
K
S
T
F
L
R
Q
T
A
L
I
A
L
L
A
Q
V
G
S
F
V
P
A
E
E
A
H
L
P
L
F
D
G
I
Y
T
R
I
G
A
------
G
K
S
T
F
M
V
E
M
E
E
V
A
L
I
L
K
E
A
T
E
N
S
L
V
L
L
D
E
V
G
R
G
T
S
S
L
D
G
V
A
I
A
T
A
V
A
E
A
L
H
E
-
R
R
A
Y
T
L
F
A
T
H
Y
F
E
L
T
A
L
G
--
L
P
R
L
K
N
L
H
V
A
A
R
E
E
A
G
G
L
V
F
Y
H
Q
V
L
P
G
P
A
S
K
S
Y
G
V
E
V
A
A
M
A
G
L
P
K
E
V
V
A
R
A
R
A
L
L
Q
A
M
A
A
R
----------------------------------------------------------------------
14
PsiBlast_PDB
95.69
35%
-81
- C3 -
1E3M
5
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
11
PsiBlast_PDB
95.68
37%
-88
- C3 -
1NNE
7
MUTS_THEAQ
A:
[13-764]
-----
L
P
P
L
L
Q
Q
Y
V
E
L
R
D
Q
Y
P
D
Y
L
L
L
F
Q
V
G
D
F
Y
E
C
F
G
E
D
A
E
R
L
A
R
A
L
G
L
V
L
T
H
K
T
S
K
-
D
F
T
T
P
M
A
G
I
P
L
R
A
F
E
A
Y
A
E
R
L
L
K
M
G
F
R
L
A
V
A
D
Q
V
E
P
A
E
E
A
E
G
L
V
R
R
E
V
T
Q
L
L
T
P
G
T
L
L
-
Q
E
S
L
L
P
R
E
-
A
N
Y
L
A
A
I
A
T
G
D
--
G
W
G
L
A
F
L
D
V
S
T
G
E
F
K
G
T
V
L
K
S
K
S
A
L
Y
D
E
L
F
R
H
R
P
A
E
V
L
L
A
P
E
L
L
E
N
G
A
F
-
L
D
E
-
F
-------------
R
K
R
F
P
V
M
L
S
E
A
P
F
E
P
E
------
G
E
G
------
P
L
A
L
R
----------
R
A
R
G
A
L
L
A
Y
A
Q
R
T
Q
G
G
A
L
S
-
L
Q
P
F
R
F
Y
D
P
G
A
F
M
R
L
P
E
A
T
L
R
A
L
E
V
F
E
P
L
R
G
Q
D
---
T
L
F
S
V
L
D
E
T
R
T
A
P
G
R
R
L
L
Q
S
W
L
R
H
P
L
L
D
R
G
P
L
E
A
R
L
D
R
V
E
G
F
V
R
E
G
A
L
R
E
G
V
R
R
L
L
Y
R
L
A
D
L
E
R
L
A
T
R
L
E
L
G
R
A
S
P
K
D
L
G
A
L
R
R
S
L
Q
I
L
P
E
L
R
A
L
L
G
E
E
V
G
L
P
D
L
S
P
L
K
E
E
L
E
A
----------
A
L
V
E
D
P
P
L
K
V
S
E
G
G
L
I
R
E
G
Y
D
P
D
L
D
A
L
R
A
A
H
R
E
G
V
A
Y
F
L
E
L
E
E
R
E
R
E
R
T
G
I
P
T
L
K
V
G
Y
N
A
V
F
G
Y
Y
L
E
V
T
R
P
Y
Y
E
R
V
P
K
E
Y
R
P
V
Q
T
L
K
D
R
Q
R
Y
T
L
P
E
M
K
E
K
E
R
E
V
Y
R
L
E
A
L
I
R
R
R
E
E
E
V
F
L
E
V
R
E
R
A
K
R
Q
A
E
A
L
R
E
A
A
R
I
L
A
E
L
D
V
Y
A
A
L
A
E
V
A
V
R
Y
G
Y
V
R
P
R
F
G
D
R
--
L
Q
I
R
A
G
R
H
P
V
V
E
R
R
T
---
E
F
V
P
N
D
L
--
E
M
A
H
E
L
V
L
I
T
G
P
N
M
A
G
K
S
T
F
L
R
Q
T
A
L
I
A
L
L
A
Q
V
G
S
F
V
P
A
E
E
A
H
L
P
L
F
D
G
I
Y
T
R
I
G
A
------
G
K
S
T
F
M
V
E
M
E
E
V
A
L
I
L
K
E
A
T
E
N
S
L
V
L
L
D
E
V
G
R
G
T
S
S
L
D
G
V
A
I
A
T
A
V
A
E
A
L
H
E
R
-
R
A
Y
T
L
F
A
T
H
Y
F
E
L
T
A
L
G
--
L
P
R
L
K
N
L
H
V
A
A
R
E
E
A
G
G
L
V
F
Y
H
Q
V
L
P
G
P
A
S
K
S
Y
G
V
E
V
A
A
M
A
G
L
P
K
E
V
V
A
R
A
R
A
L
L
Q
A
M
A
A
R
----------------------------------------------------------------------
23
PsiBlast_CBE
95.65
36%
-83
- C3 -
1W7A
4
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
A
A
D
D
--
S
G
R
S
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
3
PsiBlast_PDB
95.46
36%
-83
- C3 -
1OH7
4
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
2
PsiBlast_PDB
95.46
36%
-82
- C3 -
1OH6
4
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
9
PsiBlast_PDB
95.42
36%
-83
- C3 -
1WB9
4
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
T
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
4
PsiBlast_PDB
95.37
36%
-79
- C3 -
1OH8
4
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
28
PsiBlast_CBE
95.33
36%
-84
- C3 -
1WBD
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
Q
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
5
PsiBlast_PDB
95.32
36%
-84
- C3 -
2WTU
5
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
A
A
D
--------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
29
PsiBlast_CBE
95.23
36%
-83
- C3 -
3K0S
2
MUTS_ECOLI
B:
[17-797]
-------------
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
-----------
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
---------
S
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
N
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
1
PsiBlast_PDB
95.20
36%
-84
- C3 -
1OH5
4
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
6
PsiBlast_PDB
95.05
36%
-82
- C3 -
3ZLJ
2
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
------------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
---------
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
32
PsiBlast_CBE
94.80
37%
-83
- C3 -
1NNE
8
MUTS_THEAQ
B:
[13-764]
-----
L
P
P
L
L
Q
Q
Y
V
E
L
R
D
Q
Y
P
D
Y
L
L
L
F
Q
V
G
D
F
Y
E
C
F
G
E
D
A
E
R
L
A
R
A
L
G
L
V
L
T
H
K
T
S
K
-
D
F
T
T
P
M
A
G
I
P
L
R
A
F
E
A
Y
A
E
R
L
L
K
M
G
F
R
L
A
V
A
D
Q
V
E
P
------
L
V
R
R
E
V
T
Q
L
L
T
P
G
T
L
L
-
Q
E
S
L
L
P
R
E
-
A
N
Y
L
A
A
I
A
T
G
D
--
G
W
G
L
A
F
L
D
V
S
T
G
E
F
K
G
T
V
L
K
S
K
S
A
L
Y
D
E
L
F
R
H
R
P
A
E
V
L
L
A
P
E
L
L
E
N
G
A
F
-
L
D
E
-
F
-------------
R
K
R
F
P
V
M
L
S
E
A
P
F
E
P
E
------
G
E
G
------
P
L
A
L
R
----------
R
A
R
G
A
L
L
A
Y
A
Q
R
T
Q
G
G
A
L
S
-
L
Q
P
F
R
F
Y
D
P
G
A
F
M
R
L
P
E
A
T
L
R
A
L
E
V
F
E
P
L
R
G
Q
D
---
T
L
F
S
V
L
D
E
T
R
T
A
P
G
R
R
L
L
Q
S
W
L
R
H
P
L
L
D
R
G
P
L
E
A
R
L
D
R
V
E
G
F
V
R
E
G
A
L
R
E
G
V
R
R
L
L
Y
R
L
A
D
L
E
R
L
A
T
R
L
E
L
G
R
A
S
P
K
D
L
G
A
L
R
R
S
L
Q
I
L
P
E
L
R
A
L
L
G
E
E
V
G
L
P
D
L
S
P
L
K
E
E
L
E
A
----------
A
L
V
E
D
P
P
L
K
V
S
E
G
G
L
I
R
E
G
Y
D
P
D
L
D
A
L
R
A
A
H
R
E
G
V
A
Y
F
L
E
L
E
E
R
E
R
E
R
T
G
I
P
T
L
K
V
G
Y
N
A
V
F
G
Y
Y
L
E
V
T
R
P
Y
Y
E
R
V
P
K
E
Y
R
P
V
Q
T
L
K
D
R
Q
R
Y
T
L
P
E
M
K
E
K
E
R
E
V
Y
R
L
E
A
L
I
R
R
R
E
E
E
V
F
L
E
V
R
E
R
A
K
R
Q
A
E
A
L
R
E
A
A
R
I
L
A
E
L
D
V
Y
A
A
L
A
E
V
A
V
R
Y
G
Y
V
R
P
R
F
G
D
R
--
L
Q
I
R
A
G
R
H
P
V
V
E
R
R
T
---
E
F
V
P
N
D
L
--
E
M
A
H
E
L
V
L
I
T
G
P
N
M
A
G
K
S
T
F
L
R
Q
T
A
L
I
A
L
L
A
Q
V
G
S
F
V
P
A
E
E
A
H
L
P
L
F
D
G
I
Y
T
R
I
G
A
S
D
D
L
A
G
G
K
S
T
F
M
V
E
M
E
E
V
A
L
I
L
K
E
A
T
E
N
S
L
V
L
L
D
E
V
G
R
G
T
S
S
L
D
G
V
A
I
A
T
A
V
A
E
A
L
H
E
R
-
R
A
Y
T
L
F
A
T
H
Y
F
E
L
T
A
L
G
--
L
P
R
L
K
N
L
H
V
A
A
R
E
E
A
G
G
L
V
F
Y
H
Q
V
L
P
G
P
A
S
K
S
Y
G
V
E
V
A
A
M
A
G
L
P
K
E
V
V
A
R
A
R
A
L
L
Q
A
M
A
A
R
----------------------------------------------------------------------
24
PsiBlast_CBE
94.67
36%
-85
- C3 -
1OH8
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
25
PsiBlast_CBE
94.64
36%
-85
- C3 -
1OH7
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
10
PsiBlast_PDB
94.64
36%
-85
- C3 -
1WBB
4
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
A
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
26
PsiBlast_CBE
94.45
36%
-84
- C3 -
1OH6
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
31
PsiBlast_CBE
94.43
36%
-85
- C3 -
1WBB
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
A
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
27
PsiBlast_CBE
93.76
36%
-86
- C3 -
1OH5
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
21
PsiBlast_CBE
93.05
36%
-83
- C3 -
3ZLJ
2
MUTS_ECOLI
B:
[26-798]
---------------------
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
--------------------
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
------------
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
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Q
L
P
E
L
R
A
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-
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T
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A
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V
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A
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R
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K
M
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A
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L
R
D
L
L
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A
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D
T
P
P
V
L
V
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D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
A
A
D
D
L
A
S
G
R
S
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------
22
PsiBlast_CBE
90.86
36%
-81
- C3 -
2WTU
4
MUTS_ECOLI
B:
[36-798]
-------------------------------
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
------------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
---------------
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
-
D
N
L
L
A
A
I
-
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
-
E
---
G
-------------
R
R
G
L
R
R
R
P
L
---
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
GTR
D
L
V
G
F
G
V
E
N
A
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
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Q
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A
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A
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Y
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R
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G
L
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V
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Y
Y
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L
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K
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A
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L
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L
L
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L
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V
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V
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N
-
E
P
F
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A
N
P
L
N
L
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R
R
M
L
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T
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P
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M
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A
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A
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L
A
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A
L
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A
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F
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L
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L
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M
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A
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V
H
L
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A
L
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G
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A
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A
A
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S
Y
G
L
A
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A
A
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K
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I
K
R
A
R
Q
K
L
R
E
L
E
S
I
S
---------------------------------------------------------------------