@TOME V2.3
(Nov 2016)
Ref.
-
-
Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : bsu00180: (2016-06-01 )
MTQDELYMKEAIKEAKKAEEKGEVPIGAVLVINGEIIARAHNLRETEQRSIAHAEMLVIDEACKALGTWRLEGATLYVTLEPCPMCAGAVVLSRVEKVVFGAFDPKGGCSGTLMNLLQEERFNHQAEVVSGVLEEECGGMLSAFFRELRKKKKAARKNLSE
Atome Classification :
(22 SA)
.........10.......--.20-..-..--.....30........40........50........60........70........80..------......90........100.......110.......120-.......130.......140.......150.......160------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
(Atome)
(Ident)
(Tito)
(Num)
(Ligand)
(Uniprot)
M
T
Q
D
E
L
Y
M
K
E
A
I
K
E
A
K
K
A--EE--KG-EV--P
I
G
A
V
L
V
IN
G
E
I
I
A
RAHNL
R
E
T
E
Q
R
S
I
A
H
A
E
M
L
V
I
D
E
A
C
K
A
LGT
W
R
LEG
A
T
L
Y
V
T
LEPC------PM
C
A
G
A
V
V
L
S
R
V
E
K
V
V
F
GAFDPKGG
C
S
G
T
L
M
N
L
L
Q
E
E
RF-N
H
Q
A
E
V
V
S
G
V
L
E
E
EC
G
G
M
L
S
A
F
F
R
E
L
R
K
K
K
K
A
A
RKNLSE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
23
PsiBlast_CBE
96.12
61%
-110
- C5 -
2B3J
5
TADA_STAAM
B:
[4-154]
M
T
N
D
I
Y
F
M
T
L
A
I
E
E
A
K
K
A
--
A
Q
--
L
G
-
E
V
--
P
I
G
A
I
I
T
K
D
D
E
V
I
A
R
A
H
N
L
R
E
T
L
Q
Q
P
T
A
H
A
E
H
I
A
I
E
R
A
A
K
V
L
G
S
W
R
L
E
G
C
T
L
Y
V
T
L
E
P
C
------
V
M
C
A
G
T
I
V
M
S
R
I
P
R
V
V
Y
G
A
D
D
P
K
G
G
C
S
G
S
L
M
N
L
L
Q
Q
S
N
F
-
N
H
R
A
I
V
D
K
G
V
L
K
E
A
C
S
T
L
L
T
T
F
F
K
N
L
R
A
N
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
75
HHSearch
94.65
61%
-108
- C5 -
2B3J
5
TADA_STAAM
A:
[4-154]
M
T
N
D
I
Y
F
M
T
L
A
I
E
E
A
K
K
A
--
A
Q
--
L
G
-
E
V
--
P
I
G
A
I
I
T
K
D
D
E
V
I
A
R
A
H
N
L
R
E
T
L
Q
Q
P
T
A
H
A
E
H
I
A
I
E
R
A
A
K
V
L
G
S
W
R
L
E
G
C
T
L
Y
V
T
L
E
P
C
------
V
M
C
A
G
T
I
V
M
S
R
I
P
R
V
V
Y
G
A
D
D
P
K
G
G
C
S
G
S
L
M
N
L
L
Q
Q
S
N
F
-
N
H
R
A
I
V
D
K
G
V
L
K
E
A
C
S
T
L
L
T
T
F
F
K
N
L
R
A
N
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
21
PsiBlast_CBE
94.65
61%
-105
- C5 -
2B3J
4
TADA_STAAM
D:
[6-155]
--
N
D
I
Y
F
M
T
L
A
I
E
E
A
K
K
A
--
A
Q
--
L
G
-
E
V
--
P
I
G
A
I
I
T
K
D
D
E
V
I
A
R
A
H
N
L
R
E
T
L
Q
Q
P
T
A
H
A
E
H
I
A
I
E
R
A
A
K
V
L
G
S
W
R
L
E
G
C
T
L
Y
V
T
L
E
P
C
------
V
M
C
A
G
T
I
V
M
S
R
I
P
R
V
V
Y
G
A
D
D
P
K
G
G
C
S
G
S
L
M
N
L
L
Q
Q
S
N
F
-
N
H
R
A
I
V
D
K
G
V
L
K
E
A
C
S
T
L
L
T
T
F
F
K
N
L
R
A
N
K
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
1
PsiBlast_PDB
94.65
61%
-108
- C5 -
2B3J
5
TADA_STAAM
A:
[4-154]
M
T
N
D
I
Y
F
M
T
L
A
I
E
E
A
K
K
A
--
A
Q
--
L
G
-
E
V
--
P
I
G
A
I
I
T
K
D
D
E
V
I
A
R
A
H
N
L
R
E
T
L
Q
Q
P
T
A
H
A
E
H
I
A
I
E
R
A
A
K
V
L
G
S
W
R
L
E
G
C
T
L
Y
V
T
L
E
P
C
------
V
M
C
A
G
T
I
V
M
S
R
I
P
R
V
V
Y
G
A
D
D
P
K
G
G
C
S
G
S
L
M
N
L
L
Q
Q
S
N
F
-
N
H
R
A
I
V
D
K
G
V
L
K
E
A
C
S
T
L
L
T
T
F
F
K
N
L
R
A
N
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
22
PsiBlast_CBE
94.62
61%
-111
- C5 -
2B3J
5
TADA_STAAM
C:
[4-155]
M
T
N
D
I
Y
F
M
T
L
A
I
E
E
A
K
K
A
--
A
Q
--
L
G
-
E
V
--
P
I
G
A
I
I
T
K
D
D
E
V
I
A
R
A
H
N
L
R
E
T
L
Q
Q
P
T
A
H
A
E
H
I
A
I
E
R
A
A
K
V
L
G
S
W
R
L
E
G
C
T
L
Y
V
T
L
E
P
C
------
V
M
C
A
G
T
I
V
M
S
R
I
P
R
V
V
Y
G
A
D
D
P
K
G
G
C
S
G
S
L
M
N
L
L
Q
Q
S
N
F
-
N
H
R
A
I
V
D
K
G
V
L
K
E
A
C
S
T
L
L
T
T
F
F
K
N
L
R
A
N
K
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2
PsiBlast_PDB
87.80
45%
-104
- C5 -
2NX8
2
TADA_STRP6
A:
[19-173]
------
F
M
Q
E
A
L
K
E
S
E
K
S
--
L
Q
--
K
A
-
E
I
--
P
I
G
C
V
I
V
K
D
G
E
I
I
G
R
G
H
N
A
R
E
E
S
N
Q
A
I
M
H
A
E
M
M
A
I
N
E
A
N
A
H
E
G
N
W
R
L
L
D
T
T
L
F
V
T
I
E
P
C
------
V
M
C
S
G
A
I
G
L
A
R
I
P
H
V
I
Y
G
A
S
N
Q
K
F
G
G
V
D
S
L
Y
Q
I
L
T
D
E
R
L
-
N
H
R
V
Q
V
E
R
G
L
L
A
A
D
C
A
N
I
M
Q
T
F
F
R
Q
G
R
E
R
K
K
I
A
K
H
L
I
K
E
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
73
HHSearch
87.40
45%
-102
- C5 -
2NX8
2
TADA_STRP6
A:
[14-167]
-
E
E
Q
T
Y
F
M
Q
E
A
L
K
E
S
E
K
S
--
L
Q
--
K
A
-
E
I
--
P
I
G
C
V
I
V
K
D
G
E
I
I
G
R
G
H
N
A
R
E
E
S
N
Q
A
I
M
H
A
E
M
M
A
I
N
E
A
N
A
H
E
G
N
W
R
L
L
D
T
T
L
F
V
T
I
E
P
C
------
V
M
C
S
G
A
I
G
L
A
R
I
P
H
V
I
Y
G
A
S
N
Q
K
F
G
G
V
D
S
L
Y
Q
I
L
T
D
E
R
L
-
N
H
R
V
Q
V
E
R
G
L
L
A
A
D
C
A
N
I
M
Q
T
F
F
R
Q
G
R
E
R
K
K
I
A
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
27
PsiBlast_CBE
87.16
44%
-113
- C5 -
1Z3A
1
TADA_ECOLI
B:
[10-158]
----
E
Y
W
M
R
H
A
L
T
L
A
K
R
A
--
W
D
--
E
R
-
E
V
--
P
V
G
A
V
L
V
H
N
N
R
V
I
G
E
G
W
N
R
P
I
G
R
H
D
P
T
A
H
A
E
I
M
A
L
R
Q
G
G
L
V
M
Q
N
Y
R
L
I
D
A
T
L
Y
V
T
L
E
P
C
------
V
M
C
A
G
A
M
I
H
S
R
I
G
R
V
V
F
G
A
R
D
A
K
T
G
A
A
G
S
L
M
D
V
L
H
H
P
G
M
-
N
H
R
V
E
I
T
E
G
I
L
A
D
E
C
A
A
L
L
S
D
F
F
R
M
R
R
Q
E
I
K
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
72
HHSearch
87.13
43%
-106
* C5 *
1Z3A
1
TADA_ECOLI
A:
[7-158]
-
F
S
H
E
Y
W
M
R
H
A
L
T
L
A
K
R
A
--
W
D
--
E
R
-
E
V
--
P
V
G
A
V
L
V
H
N
N
R
V
I
G
E
G
W
N
R
P
I
G
R
H
D
P
T
A
H
A
E
I
M
A
L
R
Q
G
G
L
V
M
Q
N
Y
R
L
I
D
A
T
L
Y
V
T
L
E
P
C
------
V
M
C
A
G
A
M
I
H
S
R
I
G
R
V
V
F
G
A
R
D
A
K
T
G
A
A
G
S
L
M
D
V
L
H
H
P
G
M
-
N
H
R
V
E
I
T
E
G
I
L
A
D
E
C
A
A
L
L
S
D
F
F
R
M
R
R
Q
E
I
K
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
24
PsiBlast_CBE
87.13
52%
-114
- C5 -
1WWR
1
TADA_AQUAE
D:
[24-167]
----
E
Y
F
L
K
V
A
L
R
E
A
K
R
A
--
F
E
--
K
G
-
E
V
--
P
V
G
A
I
I
V
K
E
G
E
I
I
S
K
A
H
N
S
V
E
E
L
K
D
P
T
A
H
A
E
M
L
A
I
K
E
A
C
R
R
L
N
T
K
Y
L
E
G
C
E
L
Y
V
T
L
E
P
C
------
I
M
C
S
Y
A
L
V
L
S
R
I
E
K
V
I
F
S
A
L
D
K
K
H
G
G
V
V
S
V
F
N
I
L
D
E
P
T
L
-
N
H
R
V
K
W
E
Y
Y
P
L
-
E
E
A
S
E
L
L
S
E
F
F
K
K
L
R
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
76
HHSearch
87.06
51%
-107
- C5 -
1WWR
1
TADA_AQUAE
A:
[21-168]
-
M
G
K
E
Y
F
L
K
V
A
L
R
E
A
K
R
A
--
F
E
--
K
G
-
E
V
--
P
V
G
A
I
I
V
K
E
G
E
I
I
S
K
A
H
N
S
V
E
E
L
K
D
P
T
A
H
A
E
M
L
A
I
K
E
A
C
R
R
L
N
T
K
Y
L
E
G
C
E
L
Y
V
T
L
E
P
C
------
I
M
C
S
Y
A
L
V
L
S
R
I
E
K
V
I
F
S
A
L
D
K
K
H
G
G
V
V
S
V
F
N
I
L
D
E
P
T
L
-
N
H
R
V
K
W
E
Y
Y
P
L
-
E
E
A
S
E
L
L
S
E
F
F
K
K
L
R
N
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
25
PsiBlast_CBE
86.42
52%
-109
- C5 -
1WWR
1
TADA_AQUAE
C:
[24-167]
----
E
Y
F
L
K
V
A
L
R
E
A
K
R
A
--
F
E
--
K
G
-
E
V
--
P
V
G
A
I
I
V
K
E
G
E
I
I
S
K
A
H
N
S
V
E
E
L
K
D
P
T
A
H
A
E
M
L
A
I
K
E
A
C
R
R
L
N
T
K
Y
L
E
G
C
E
L
Y
V
T
L
E
P
C
------
I
M
C
S
Y
A
L
V
L
S
R
I
E
K
V
I
F
S
A
L
D
K
K
H
G
G
V
V
S
V
F
N
I
L
D
E
P
T
L
-
N
H
R
V
K
W
E
Y
Y
P
L
-
E
E
A
S
E
L
L
S
E
F
F
K
K
L
R
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
26
PsiBlast_CBE
86.36
52%
-114
- C5 -
1WWR
1
TADA_AQUAE
B:
[24-167]
----
E
Y
F
L
K
V
A
L
R
E
A
K
R
A
--
F
E
--
K
G
-
E
V
--
P
V
G
A
I
I
V
K
E
G
E
I
I
S
K
A
H
N
S
V
E
E
L
K
D
P
T
A
H
A
E
M
L
A
I
K
E
A
C
R
R
L
N
T
K
Y
L
E
G
C
E
L
Y
V
T
L
E
P
C
------
I
M
C
S
Y
A
L
V
L
S
R
I
E
K
V
I
F
S
A
L
D
K
K
H
G
G
V
V
S
V
F
N
I
L
D
E
P
T
L
-
N
H
R
V
K
W
E
Y
Y
P
L
-
E
E
A
S
E
L
L
S
E
F
F
K
K
L
R
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
4
PsiBlast_PDB
86.33
44%
-110
- C5 -
1Z3A
1
TADA_ECOLI
A:
[10-158]
----
E
Y
W
M
R
H
A
L
T
L
A
K
R
A
--
W
D
--
E
R
-
E
V
--
P
V
G
A
V
L
V
H
N
N
R
V
I
G
E
G
W
N
R
P
I
G
R
H
D
P
T
A
H
A
E
I
M
A
L
R
Q
G
G
L
V
M
Q
N
Y
R
L
I
D
A
T
L
Y
V
T
L
E
P
C
------
V
M
C
A
G
A
M
I
H
S
R
I
G
R
V
V
F
G
A
R
D
A
K
T
G
A
A
G
S
L
M
D
V
L
H
H
P
G
M
-
N
H
R
V
E
I
T
E
G
I
L
A
D
E
C
A
A
L
L
S
D
F
F
R
M
R
R
Q
E
I
K
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
5
PsiBlast_PDB
86.19
45%
-108
- C5 -
3OCQ
1
?
A:
[20-162]
----
E
Y
W
M
R
H
A
L
T
L
A
K
R
A
--
W
D
--
E
R
-
E
V
--
P
V
G
A
V
L
V
H
N
H
R
V
I
G
E
G
W
N
R
P
I
G
R
H
D
P
T
A
H
A
E
I
M
A
L
R
Q
G
G
L
V
L
Q
N
Y
R
L
L
D
T
T
L
Y
V
T
L
E
P
C
------
V
M
C
A
G
A
M
V
H
S
R
I
G
R
V
V
F
G
A
R
D
A
K
T
G
A
A
G
S
L
I
D
V
L
H
H
P
G
M
-
N
H
R
V
E
I
I
E
G
V
L
R
D
E
C
A
T
L
L
S
D
F
F
R
M
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
3
PsiBlast_PDB
86.11
52%
-111
- C5 -
1WWR
1
TADA_AQUAE
A:
[24-167]
----
E
Y
F
L
K
V
A
L
R
E
A
K
R
A
--
F
E
--
K
G
-
E
V
--
P
V
G
A
I
I
V
K
E
G
E
I
I
S
K
A
H
N
S
V
E
E
L
K
D
P
T
A
H
A
E
M
L
A
I
K
E
A
C
R
R
L
N
T
K
Y
L
E
G
C
E
L
Y
V
T
L
E
P
C
------
I
M
C
S
Y
A
L
V
L
S
R
I
E
K
V
I
F
S
A
L
D
K
K
H
G
G
V
V
S
V
F
N
I
L
D
E
P
T
L
-
N
H
R
V
K
W
E
Y
Y
P
L
-
E
E
A
S
E
L
L
S
E
F
F
K
K
L
R
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
28
PsiBlast_CBE
82.21
44%
-104
- C5 -
2A8N
1
TADA_AGRFC
B:
[4-141]
---
R
T
H
F
M
E
L
A
L
V
E
A
R
S
A
--
G
E
--
R
D
-
E
V
--
P
I
G
A
V
L
V
L
D
G
R
V
I
A
R
S
G
N
R
T
R
E
L
N
D
V
T
A
H
A
E
I
A
V
I
R
M
A
C
E
A
L
G
Q
E
R
L
P
G
A
D
L
Y
V
T
L
E
P
C
------
T
M
C
A
A
A
I
S
F
A
R
I
R
R
L
Y
Y
G
A
Q
D
P
K
G
G
A
V
E
S
G
V
R
F
F
S
Q
P
T
C
-
H
H
A
P
D
V
Y
S
G
L
A
E
S
E
S
A
E
I
L
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
87
HHSearch
81.52
40%
-120
- C5 -
2HXV
4
?
A:
[12-158]
-
H
M
Y
E
T
F
M
K
R
A
I
E
L
A
K
K
G
--
L
G
RV
N
P
-
N
P
--
P
V
G
A
V
V
V
K
D
G
R
I
I
A
E
G
F
H
P
Y
----
F
G
G
P
H
A
E
R
M
A
I
E
S
A
R
K
K
--
G
E
D
L
R
G
A
T
L
I
V
T
L
E
P
C
DHHGKT
P
P
C
T
D
L
I
I
E
S
G
I
K
T
V
V
I
G
T
R
D
P
N
P
V
S
-
G
N
G
V
E
K
F
R
-----
N
H
G
I
E
V
I
E
G
V
L
E
E
E
V
K
K
L
C
E
F
F
I
T
Y
V
T
K
K
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
13
PsiBlast_PDB
79.65
36%
-112
- C5 -
2HXV
5
?
A:
[15-159]
----
E
T
F
M
K
R
A
I
E
L
A
K
K
G
LG
R
V
--
N
P
-
N
P
--
P
V
G
A
V
V
V
K
D
G
R
I
I
A
E
G
F
H
----
P
Y
F
G
G
P
H
A
E
R
M
A
I
E
S
A
R
K
K
--
G
E
D
L
R
G
A
T
L
I
V
T
L
E
P
C
DHHGKT
P
P
C
T
D
L
I
I
E
S
G
I
K
T
V
V
I
G
T
R
D
P
N
P
-
V
S
G
N
G
V
-----
E
K
F
R
N
H
G
I
E
V
I
E
G
V
L
E
E
E
V
K
K
L
C
E
F
F
I
T
Y
V
T
K
K
R
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
94
Fugue
78.93
31%
-110
- C5 -
2B3Z
1
RIBD_BACSU
A:
[13-371]
--
M
E
E
Y
Y
M
K
L
A
L
D
L
A
K
Q
G
--
E
G
--
Q
T
-
E
S
N
P
L
V
G
A
V
V
V
K
D
G
Q
I
V
G
M
G
A
----
H
L
K
Y
G
E
A
H
A
E
V
H
A
I
H
M
A
G
A
H
A
-----
E
G
A
D
I
Y
V
T
L
E
P
C
SHYGKT
P
P
C
A
E
L
I
I
N
S
G
I
K
R
V
F
V
A
M
R
D
P
N
P
L
V
A
G
R
G
I
S
M
M
K
E
A
G
I
-----
E
V
R
E
G
I
L
A
D
Q
A
E
R
L
N
E
K
F
L
H
F
M
R
T
G
L
P
Y
V
T
L
K
A
A
A
S
LDGKIATSTGDSKWITS
E
A
A
R
Q
D
A
Q
Q
Y
R
K
T
H
Q
S
I
L
V
G
V
G
T
V
K
A
DNPSLTCRLPNVTKQ
P
V
R
V
I
L
D
TVLSIPEDAKVICDQI
A
P
T
W
I
F
T
T
ARAD
E
E
K
K
K
R
L
S
A
F
G
V
N
I
F
T
L
ETERIQ
I
P
D
V
L
K
I
L
A
E
E
GIMSVYVEGG
S
A
V
H
G
S
F
V
K
E
GCFQ
E
I
I
F
Y
F
A
P
K
L
I
G
G
THAPSL
I
S
G
EGFQSMKD
V
P
L
L
Q
FT
D
I
T
Q
I
GR
D
I
K
L
T
A
K
P
T
32
PsiBlast_CBE
69.47
40%
-123
- C5 -
1WKQ
3
GUAD_BACSU
B:
[12-110]
----
E
T
F
L
K
R
A
V
T
L
A
C
E
G
--
V
N
--
A
G
I
G
G
--
P
F
G
A
V
I
V
K
D
G
A
I
I
A
E
G
Q
N
N
V
T
T
S
N
D
P
T
A
H
A
E
V
T
A
I
R
K
A
C
K
V
L
G
A
Y
Q
L
D
D
C
I
L
Y
T
S
C
E
P
C
------
P
M
C
L
G
A
I
Y
W
A
R
P
K
A
V
F
Y
A
A
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
8
PsiBlast_PDB
68.86
40%
-119
- C5 -
1WKQ
3
GUAD_BACSU
A:
[12-110]
----
E
T
F
L
K
R
A
V
T
L
A
C
E
G
--
V
N
--
A
G
I
G
G
--
P
F
G
A
V
I
V
K
D
G
A
I
I
A
E
G
Q
N
N
V
T
T
S
N
D
P
T
A
H
A
E
V
T
A
I
R
K
A
C
K
V
L
G
A
Y
Q
L
D
D
C
I
L
Y
T
S
C
E
P
C
------
P
M
C
L
G
A
I
Y
W
A
R
P
K
A
V
F
Y
A
A
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------