@TOME V2.3
(Nov 2016)
Ref.
-
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Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : bsu04890: (2016-06-08 )
MFNINLLNQARLESDLDSLGCLDIAEELIEKMMEDVFYSDVAQRIIVQTLAVINNANAKLQATFNILEDEDMETDERSVEFEGISYYE
Atome Classification :
(20 SA)
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------.......-----..10---.....-.--------..-20.....-----...30.....-...40-----.......-------------.-50..-......60...--.....70--.-..-......-80.......-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
(Atome)
(Ident)
(Tito)
(Num)
(Ligand)
(Uniprot)
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------M
F
N
I
N
L
L
-----
N
Q
A
R
---
L
E
S
D
L
-
D
--------SL-
G
C
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D
I
A
E
-----
E
L
I
E
K
M
M
E
D
V
-
F
Y
S
D
V
-----
A
Q
R
I
I
V
Q
-------------
T
-
L
A
V
I-NNAN
A
K
L
Q
A
T
F
--NILEDE---D-ME-TDER
S
V
-
E
FEGISYYE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
16
Fugue
74.88
16%
-194
- C5 -
4IWS
-
FDC_PSEAE
A:
[23-515]
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------M
N
R
S
A
L
D
F
R
H
F
V
D
H
L
R
R
Q
GD
L
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V
HTEVDANL
E
I
G
A
I
T
R
R
V
Y
E
R
RA
P
A
P
L
F
HNIRDSLPG
A
R
V
L
G
APAGLRADRARAH
S
R
L
A
L
H
F
GLPEHSG
P
R
D
I
V
A
M
L
R
A
A
M
R
A
E
P
I
APRRLERGPVQE
N
V
W
L
GEQVDLTRFPVPLLHEQDGGRYFGTY
G
F
H
V
V
Q
T
PDGS
W
D
S
W
S
V
G
R
L
M
L
V
D
RN
T
L
A
G
P
T
I
PTQ
H
I
G
I
I
R
E
Q
W
R
R
L
GK
P
T
P
W
A
M
A
L
GAP
P
A
A
L
A
A
A
G
MPLPEGVSEAGYVGALVG
E
P
V
E
V
V
R
T
QTNGLWVPANTE
I
V
L
E
G
E
ISLDETALEGPMGEYHGYSFP
I
G
K
P
Q
P
L
F
H
V
H
A
L
S
F
R
DQ
P
I
L
P
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C
VAGTPPE
E
N
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M
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A
Q
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L
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A
Q
N
A
GLPVDMVWCSYEAATC
W
A
V
L
S
I
D
V
Q
-----
R
L
A
A
---
L
G
T
D
-------------------
A
A
-----
A
F
A
A
R
V
A
E
T
V
-
F
G
S
H
A
-----
G
H
L
V
P
-
K
-------------
L
-
I
L
V
G
-
N
D
I
D
V
T
E
I
D
Q
V
--
V
W
A
L
A
T
---
R
-
A
H
-
P
L
H
D
H
F
-
A
F
P
Q
I
R
D
F
P
MVPYLD
A
E
D
K
A
R
GSGG
R
L
V
I
N
CLY
P
E
Q
F
A
GQMRAATASFRHAYP
T
A
L
R
R
R
V
E
E
R
W
SDYGF-------------------------------------------------------------------------------------------------------------------------------------------------------------
21
Fugue
71.71
20%
-144
- C4 -
1UFW
-
A:
[1-95]
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GS
S
G
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S
G
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S
F
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P
LDA
T
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Q
-----
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L
---
E
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N
-----------------
E
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-----
D
L
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Q
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L
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S
Y
G
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-----
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-------------
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M
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V
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T
F
A
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--
H
S
A
L
--
S
V
L
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V
D
---
G
-
M
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K
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P
S
S
G
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
12
Fugue
67.90
25%
-125
- C4 -
4MT1
-
?
A:
[2-1040]
A
K
F
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P
I
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A
W
V
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I
I
A
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LPVSQYPSVA
A
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L
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A
I
Y
PGAS
A
Q
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M
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SV
L
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N
MNGVEGLD
Y
M
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T
S
A
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SSG
S
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F
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PDTD
E
N
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LSTLP
A
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KARSNFL
M
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VPELQRIEGVG
Q
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F
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T
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Q
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A
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VILRANTDG
S
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V
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L
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M
EDYS
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T
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V
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NSGNA
M
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Y
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M
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QNIRYT
L
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L
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G
F
A
F
I
S
Y
M
GMSIN
V
L
T
M
F
A
M
I
L
V
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GIVV
D
D
A
I
V
V
V
E
N
V
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R
I
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A
G
EGLP
P
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E
A
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K
A
M
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I
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A
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I
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L
I
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F
VPLAMFSG
A
A
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N
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Q
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A
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T
M
A
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S
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A
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S
A
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L
A
L
TLT
P
A
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A
TG
F
F
G
W
F
N
K
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G
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V
A
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L
R
K
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F
R
M
M
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V
Y
I
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L
A
V
V
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L
F
M
R
L
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PTEDQG
F
V
M
V
S
V
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L
P
A
GAT
K
E
R
T
D
A
T
L
A
Q
V
T
Q
L
A
K
S
IPEIE
N
I
I
T
V
S
GFSFSGSGQN
M
A
M
G
F
AIFKDWNERTASGSD
A
V
A
V
A
G
K
L
T
G
M
M
M
G
TLKD
G
F
G
I
A
V
V
P
P
PILELG
S
G
L
S
I
N
L
Q
D
RNNTG
H
T
A
L
L
A
K
R
N
E
L
I
Q
K
M
R
A
SGLFDPST
V
R
A
G
G
L
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D
S
P
Q
L
K
I
D
I
N
----------
R
---
A
A
A
A
A
-
Q
--------
G
I
-
S
F
A
D
I
R
T
-----
A
L
-
A
S
A
L
S
S
S
-
Y
V
S
D
F
P
N
QG
R
L
Q
R
V
M
V
Q
A
D
EDARMQPADIL
N
-
L
T
V
P
-
N
K
S
G
V
A
V
P
L
S
T
---
I
A
T
V
S
---
W
-
E
N
-
G
T
E
Q
S
V
-
R
F
N
G
Y
P
S
M
K
L
S
A
S
PATGVST
G
Q
A
M
E
A
V
Q
K
M
V
D
E
L
GGG
Y
S
F
E
W
G
G
Q
S
S
E
E
A
K
GGSQ
T
L
I
L
Y
G
L
A
V
A
A
V
F
L
V
L
A
A
L
Y
ESW
S
I
P
L
A
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L
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L
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L
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A
A
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V
T
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R
N
L
F
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G
L
LGSVPSFAND
I
Y
F
Q
V
G
F
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T
V
M
G
L
S
A
K
N
A
I
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A
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D
L
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A
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GKS
A
V
E
A
A
L
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A
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R
L
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P
I
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A
F
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PLYIAAGAS
S
A
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Q
R
A
I
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T
T
V
F
W
G
M
L
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T
L
L
S
V
F
L
V
P
L
F
Y
V
V
V
R
K
F
F
15
Fugue
64.63
21%
-80
* C5 *
4NFU
3
EDS1L_ARATH
A:
[15-633]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AFEALTGIN
G
D
L
I
T
R
S
W
S
A
S
K
Q
A
Y
L
T
ER
Y
H
K
E
E
A
GA
V
V
I
F
A
F
Q
P
S
FSEKDFFDPDNKSSFG
E
I
K
L
NRVQFPCMRKIGKG
D
V
A
T
V
N
E
A
F
L
K
N
L
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A
V
I
DPRTS
F
Q
A
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M
A
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RKQ
I
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T
GH
S
S
G
G
A
T
A
I
L
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T
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W
Y
L
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E
P
R
C
V
T
F
GAPLVGD
S
I
F
S
H
A
L
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R
E
KWSRF
F
V
N
F
V
T
RFDIVPRITLARKASVEET
L
P
H
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A
Q
L
DPRNSSVQES
E
Q
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F
Y
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A
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GSA
E
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L
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S
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Y
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R
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A
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NN
S
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H
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Y
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L
V
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M
G
M
K
L
F
N
H
L
-----
D
G
E
N
S--
I
E
S
S
L
-
N
--------
D
L
-
G
V
S
T
R
G
R
-----
Q
Y
V
Q
A
A
L
E
E
E
-
K
K
R
V
E
-----
N
Q
K
K
I
I
Q
-------------
V
-
I
Q
------
Q
E
R
F
L
K
K
L
--
A
W
I
E
D
E
---------
Y
K
P
K
C
-
Q
A
H
K
N
G
Y
Y
D
S
F
K
V
SNE
E
N
D
F
K
A
N
V
K
R
A
E
L
A
G
V
F
D
E
V
L
G
L
L
K
K
CQLPDEFEGD
I
D
W
I
K
L
A
T
R
Y
R
R
L
V
E
P
L
D
I
A
N
Y
H
R
H
LKNEDTGPYMKRGRP
T
R
Y
I
Y
A
Q
R
G
Y
E
H
H
I
L
KPNGMI
A
E
D
V
F
W
N
K
V
N
G
L
NLGLQ
L
E
E
I
Q
E
T
L
KNSGSECGSC
F
W
A
E
V
E
E
L
KGKPYEEV
E
V
R
V
K
T
L
E
G
M
L
R
E
W
I
T
A
GEVDEKEIFLEGS
T
F
R
K
W
W
I
T
LP
K
N
H
K
S
H
SPLRDY
M
M
D
E
I-----
19
Fugue
62.58
22%
-99
- C1 -
2XC8
-
?
A:
[1-121]
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
G
I
E
I
V
-----
N
R
K
A
VWY
L
T
S
E
I
-
K
E
T
ET
G
I
E
V
S
A
-
G
E
L
H
K
G
D
-----
E
E
V
F
P
V
E
E
V
S
-
F
D
L
T
P
-----
D
D
T
Y
P
V
E
-------------
Y
M
L
Y
L
H
M
N
V
Q
T
K
K
V
S
W
S
L
C
K
A
Y
L
D
G
E
G
Y
C
D
-
Y
Q
-
G
N
E
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M
Y
P
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T
V
F
P
NG
T
R
E
G
T
I
F
L
Y
E
K
EDR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
8
HHSearch
58.38
37%
-175
- C6 -
3O2T
-
SYMPK_HUMAN
A:
[28-61]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
V
D
L
L
-----
N
Q
A
A
---
L
I
T
N
D
-
S
--------
K
I
-
T
V
L
K
Q
V
Q
-----
E
L
I
I
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
13
Fugue
51.23
11%
-108
* C4 *
4CU5
-
?
A:
[186-270]
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
M
Y
K
H
T
I
V
-----
Y
-------
D
G
E
V
-
D
--------
K
I
-
S
A
T
V
V
G
W
G
Y
ND
G
K
I
L
I
C
D
I
K
D
--
Y
V
P
G
Q
-----
T
Q
N
L
Y
V
V
-------------
G
-
G
G
A
C
-
E
K
I
S
S
I
T
K
E
K
F
--
I
M
I
K
G
N
---
D
R
F
D
-
T
L
Y
K
A
L
-
D
F
I
N
R
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
17
Fugue
50.93
20%
-78
- C2 -
1C01
-
AMP1_MACIN
A:
[1-76]
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------S
A
F
T
V
W
S
G
-----
P
G
C
N
---
N
R
A
E
R
-
Y
--------
S
K
C
G
C
S
A
I
H
Q
---------
K
G
G
Y
D
F
-
S
Y
T
G
------------
Q
-------------
T
-
A
A
L
Y
-
N
Q
A
G
C
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G
V
A
H
T
--
R
F
---
G
---
S
-
S
A
-
R
A
C
N
P
F
-
G
W
K
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I
F
I
Q
C
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
6
HHSearch
47.86
25%
-105
- C4 -
2PFC
-
Y098_MYCTU
A:
[20-84]
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
P
I
A
E
-----
E
L
L
A
R
V
L
E
---
P
Y
S
C
K
-----
G
C
R
Y
L
I
D
-------------
A
-
Q
Y
S
A
-
T
E
D
S
V
L
A
Y
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N
F
--
T
I
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A
Y--
I
-
R
S
T
G
H
F
N
A
V
-
E
L
I
L
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
5
HHSearch
47.44
24%
-178
- C6 -
4O6X
-
ANK3_HUMAN
A:
[66-92]
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
D
A
---
L
T
S
V
L
-
T
--------
K
I
-
N
R
I
D
I
V
T
-----
L
L
E
G
P
I
F
D
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
20
Fugue
44.64
14%
45
- C5 -
1RP3
-
?
B:
[2-88]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
V
N
R
I
-----
E
L
S
R
---
L
I
G
L
L
L
E
--------
T
S
-
G
T
N
K
I
E
D
-----
K
V
T
L
S
K
I
A
Q
E
-
L
S
K
N
D
-----
V
E
E
------------------------
K
D
L
E
K
K
V
K
E
L
K
--
E
K
I
E
K
G
---
E
-
Y
E
-
V
S
D
E
K
V
-
V
K
G
L
I
E
F
F
T
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
14
Fugue
44.11
14%
-66
- C4 -
4OLK
2
?
A:
[4-167]
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MAKT
Q
A
E
I
N
K
R
L
D
A
Y
A
K
GTVDSPYRIKKATSYDPSFGVMEAG
A
I
D
A
D
G
Y
-----
Y
H
A
Q
----------------------
C
Q
D
L
I
T
-----
D
Y
V
L
W
L
T
D
-------------------
N
-------------
K
-
V
R
T
W
-
G
N
A
K
D
Q
I
K
Q
S
Y
--
G
----------------------
T
G
F
K
I
H
E
N
K
PS
T
V
P
K
KG
W
I
A
V
F
TSGSYQQW
G
H
I
G
I
V
YDGGNTS
T
F
T
I
L
E
Q
NWNGYANK
K
P
T
K
R
V
D
NYYGLT
H
F
I
E
I
P
V
K-------------------------------------------------------------------------------------------------------------------------------------------------
1
PsiBlast_PDB
43.11
34%
35
- C6 -
3KT4
-
TPA1_YEAST
A:
[245-329]
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
V
R
-
N
N
T
S
T
L
A
Q
I
E
S
--
N
V
L
E
D
F
---
E
-
F
P
-
K
D
E
R
N
I
L
S
F
H
E
V
K
H
F
E
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
3
HHSearch
42.67
14%
-100
- C6 -
4IMI
-
SYMPK_DROME
A:
[19-46]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
V
D
W
C
-----
N
E
L
V
---
I
A
S
P
S
-
T
--------
K
C
-
E
L
L
A
K
V
Q
-----
E
T
V
L
G
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
11
HHSearch
42.06
19%
-128
* C6 *
2YQF
-
ANK1_HUMAN
A:
[1465-1491]
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
E
N
---
L
Y
T
A
L
-
Q
--------
S
I
-
D
R
G
E
I
V
N
-----
M
L
E
G
S
G
R
Q
S
R
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
10
HHSearch
40.10
26%
-70
- C6 -
1WXP
-
A:
[78-104]
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
E
N
---
L
I
N
A
L
-
N
--------
K
S
-
G
L
S
D
L
A
E
-----
S
L
T
N
D
N
E
T
N
S
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
9
HHSearch
35.47
22%
-102
- C6 -
1FAD
-
FADD_MOUSE
A:
[159-181]
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
A
G
---
L
V
K
A
L
-
R
--------
T
C
-
R
L
N
L
V
A
D
-----
L
V
E
E
A
Q
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2
HHSearch
33.18
13%
-56
- C6 -
3OQ9
-
TNR6_MOUSE
A:
[284-307]
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Q
D
---
L
I
K
G
L
-
K
--------
K
A
-
E
C
R
R
T
L
D
-----
K
F
Q
D
M
V
Q
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
7
HHSearch
32.80
21%
196
- C6 -
3EZQ
1
TNR6_HUMAN
A:
[70-97]
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
D
T
---
L
I
K
D
L
-
K
--------
K
A
-
N
L
C
T
L
A
E
-----
K
I
Q
T
I
I
L
K
D
I
-
T
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
18
Fugue
27.25
40%
198
- C3 -
3PLX
1
PAND_CAMJE
A:
[3-27]
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
A
M
N
I
T
L
L
K
S
K
I
H
R
A
S
V
---
T
E
A
R
L
-
D
--------
Y
I
-
G
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------