@TOME V2.3
(Nov 2016)

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3D Common Core:

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Modeller Result :

Complexes Modeling
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Values color: [ Good | Correct | Middling | Bad ]Sequence color : MutatedHelixStrandConserved

Query sequence : bsu35920: (2016-07-30 )
MRNICVIGSCSMDLVVTSDKRPKAGETVLGTSFQTVPGGKGANQAVAAARLGAQVFMVGKVGDDHYGTAILNNLKANGVRTDYMEPVTHTESGTAHIVLAEGDNSIVVVKGANDDITPAYALNALEQIEKVDMVLIQQEIPEETVDEVCKYCHSHDIPIILNPAPARPLKQETIDHATYLTPNEHEASILFPELTISEALALYPAKLFITEGKQGVRYSAGSKEVLIPSFPVEPVDTTGAGDTFNAAFAVALAEGKDIEAALRFANRAASLSVCSFGAQGGMPTRNEVEELLS

Atome Classification :

(30 SA) .........10........20.......-.30.-.......40........50........60........70........80........90......-..-100........110.......120.......130.......140.......150.......160....---.--..170.......180.......190---.....-----.---...---------200-.---.....----..---------.210.......220.--......230.......240.......250..--.----.----.-..260--.........270.......280.......290.--
(Atome) (Ident) (Tito) (Num) (Ligand) (Uniprot) MRNICVIGSCSMDLVVTSDKRPKAGETV-LGTS-FQTVPGGKGANQAVAAARLGAQVFMVGKVGDDHYGTAILNNLKANGVRTDYMEPVTHTESGTAHI-VL-AE-GDNSIVVVKGANDDITPAYALNALEQIEKVDMVLIQQEIPEETVDEVCKYCHSHDIPIILNPAPA---R--PLKQETIDHATYLTPNEHEASIL-----FPELT-----I---SEA---------L---A---LYPAK----LF---------ITEGKQGVRYSAGSK--EVLIPSFPVEPVDTTGAGDTFNAAFAVALAE--G----K----D-IEA----ALRFANRAASLSVCSFGAQGGMPTRNEVEELLS--
24 PsiBlast_CBE 79.5037% -35 - C1 -4XCK ? D:[4-306] MNKLVVLGSVNADHVLQVPSFPRPGETL-HGRN-YQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRESFKLDGINTAGVKLQPNCPTGIAMI-QV-SDSGENSICISAEANAKLTAAAIEPDLAAIRDARYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPA---R--ELPDELLKCVDLITPNETEAEVLTGITVYDDSS-----A---QQA---------A---D---ALHCKGIEIVI---------ITLGSKGVWLSQNGR--GQRIPGFVVKATDTTAAGDTFNGALVTGLLQ--E----M----P-LES----AIKFAHAAAAISVTRFGAQTSIPTRAEVEAFLA--
26 PsiBlast_CBE 79.1537% -37 - C1 -4XCK ? B:[4-306] MNKLVVLGSVNADHVLQVPSFPRPGETL-HGRN-YQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRESFKLDGINTAGVKLQPNCPTGIAMI-QV-SDSGENSICISAEANAKLTAAAIEPDLAAIRDARYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPA---R--ELPDELLKCVDLITPNETEAEVLTGITVYDDSS-----A---QQA---------A---D---ALHCKGIEIVI---------ITLGSKGVWLSQNGR--GQRIPGFVVKATDTTAAGDTFNGALVTGLLQ--E----M----P-LES----AIKFAHAAAAISVTRFGAQTSIPTRAEVEAFLA--
59 HHSearch 78.8438% -36 - C1 -4XDA ? A:[4-305] MNKLVVLGSVNADHVLQVPSFPRPGETL-HGRN-YQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRESFKLDGINTAGVKLQPNCPTGIAMI-QVSDS-GENSICISAEANAKLTAAAIEPDLAAIRDARYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPA---R--ELPDELLKCVDLITPNETEAEVL-----TGITVYDDSSA---QQA---------ADALHC--KGIEI----VI---------ITLGSKGVWLSQNGR--GQRIPGFVVKATDTTAAGDTFNGALVTGLLQ--E----M----P-LES----AIKFAHAAAAISVTRFGAQTSIPTRAEVEAFL---
25 PsiBlast_CBE 78.7737% -35 - C1 -4XCK ? C:[4-306] MNKLVVLGSVNADHVLQVPSFPRPGETL-HGRN-YQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRESFKLDGINTAGVKLQPNCPTGIAMI-QV-SDSGENSICISAEANAKLTAAAIEPDLAAIRDARYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPA---R--ELPDELLKCVDLITPNETEAEVLTGITVYDDSS-----A---QQA---------A---D---ALHCKGIEIVI---------ITLGSKGVWLSQNGR--GQRIPGFVVKATDTTAAGDTFNGALVTGLLQ--E----M----P-LES----AIKFAHAAAAISVTRFGAQTSIPTRAEVEAFLA--
21 PsiBlast_CBE 78.6737% -35 - C1 -4XDA ? D:[4-306] MNKLVVLGSVNADHVLQVPSFPRPGETL-HGRN-YQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRESFKLDGINTAGVKLQPNCPTGIAMI-QV-SDSGENSICISAEANAKLTAAAIEPDLAAIRDARYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPA---R--ELPDELLKCVDLITPNETEAEVLTGITVYDDSS-----A---QQA---------A---D---ALHCKGIEIVI---------ITLGSKGVWLSQNGR--GQRIPGFVVKATDTTAAGDTFNGALVTGLLQ--E----M----P-LES----AIKFAHAAAAISVTRFGAQTSIPTRAEVEAFLA--
3 PsiBlast_PDB 78.5437% -36 - C1 -4XDA ? A:[4-306] MNKLVVLGSVNADHVLQVPSFPRPGETL-HGRN-YQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRESFKLDGINTAGVKLQPNCPTGIAMI-QV-SDSGENSICISAEANAKLTAAAIEPDLAAIRDARYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPA---R--ELPDELLKCVDLITPNETEAEVLTGITVYDDSS-----A---QQA---------A---D---ALHCKGIEIVI---------ITLGSKGVWLSQNGR--GQRIPGFVVKATDTTAAGDTFNGALVTGLLQ--E----M----P-LES----AIKFAHAAAAISVTRFGAQTSIPTRAEVEAFLA--
22 PsiBlast_CBE 78.2937% -35 - C1 -4XDA ? C:[4-306] MNKLVVLGSVNADHVLQVPSFPRPGETL-HGRN-YQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRESFKLDGINTAGVKLQPNCPTGIAMI-QV-SDSGENSICISAEANAKLTAAAIEPDLAAIRDARYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPA---R--ELPDELLKCVDLITPNETEAEVLTGITVYDDSS-----A---QQA---------A---D---ALHCKGIEIVI---------ITLGSKGVWLSQNGR--GQRIPGFVVKATDTTAAGDTFNGALVTGLLQ--E----M----P-LES----AIKFAHAAAAISVTRFGAQTSIPTRAEVEAFLA--
2 PsiBlast_PDB 77.9637% -35 - C1 -4XCK ? A:[4-306] MNKLVVLGSVNADHVLQVPSFPRPGETL-HGRN-YQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRESFKLDGINTAGVKLQPNCPTGIAMI-QV-SDSGENSICISAEANAKLTAAAIEPDLAAIRDARYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPA---R--ELPDELLKCVDLITPNETEAEVLTGITVYDDSS-----A---QQA---------A---D---ALHCKGIEIVI---------ITLGSKGVWLSQNGR--GQRIPGFVVKATDTTAAGDTFNGALVTGLLQ--E----M----P-LES----AIKFAHAAAAISVTRFGAQTSIPTRAEVEAFLA--
1 PsiBlast_PDB 77.9137% -33 * C1 *4X8F - ? A:[1-303] MNKLVVLGSVNADHVLQVPSFPRPGETL-HGRN-YQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRESFKLDGINTAGVKLQPNCPTGIAMI-QV-SDSGENSICISAEANAKLTAAAIEPDLAAIRDARYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPA---R--ELPDELLKCVDLITPNETEAEVLTGITVYDDSS-----A---QQA---------A---D---ALHCKGIEIVI---------ITLGSKGVWLSQNGR--GQRIPGFVVKATDTTAAGDTFNGALVTGLLQ--E----M----P-LES----AIKFAHAAAAISVTRFGAQTSIPTRAEVEAFLA--
23 PsiBlast_CBE 77.8537% -36 - C1 -4XDA ? B:[4-306] MNKLVVLGSVNADHVLQVPSFPRPGETL-HGRN-YQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRESFKLDGINTAGVKLQPNCPTGIAMI-QV-SDSGENSICISAEANAKLTAAAIEPDLAAIRDARYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPA---R--ELPDELLKCVDLITPNETEAEVLTGITVYDDSS-----A---QQA---------A---D---ALHCKGIEIVI---------ITLGSKGVWLSQNGR--GQRIPGFVVKATDTTAAGDTFNGALVTGLLQ--E----M----P-LES----AIKFAHAAAAISVTRFGAQTSIPTRAEVEAFLA--
30 PsiBlast_CBE 75.0036% -35 - C1 -1GQT RBSK_ECOLI D:[6-305] --SLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVN-GE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRV-----E---NDE---------D---A---AKAAQ----VLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
31 PsiBlast_CBE 74.4836% -32 - C1 -1GQT RBSK_ECOLI C:[6-305] --SLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVN-GE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRV-----E---NDE---------D---A---AKAAQ----VLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
60 HHSearch 74.0936% -33 - C1 -1RKD RBSK_ECOLI A:[4-305] AGSLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALI-FVNGE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRVEN---DEDAAKA---------AQVLHE--KGIRT----VL---------ITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
7 PsiBlast_PDB 74.0936% -28 - C1 -1RKA - RBSK_ECOLI A:[6-305] --SLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVN-GE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRV-----E---NDE---------D---A---AKAAQ----VLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
32 PsiBlast_CBE 73.6036% -32 - C1 -1GQT RBSK_ECOLI B:[6-305] --SLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVN-GE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRV-----E---NDE---------D---A---AKAAQ----VLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
8 PsiBlast_PDB 73.5736% -32 - C1 -1RKS RBSK_ECOLI A:[6-305] --SLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVN-GE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRV-----E---NDE---------D---A---AKAAQ----VLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
62 HHSearch 73.2636% -33 - C1 -2FV7 RBSK_HUMAN A:[24-330] VAAVVVVGSCMTDLVSLTSRLPKTGETI-HGHK-FFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASI-IVNNE-GQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAI--A--DLDPQFYTLSDVFCCNESEAEIL-----TGLTVGSAADA---GEA---------AL--VLLKRGCQV----VI---------ITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPN----L----S-LED----MLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTL---
10 PsiBlast_PDB 73.0836% -31 - C1 -1RKD RBSK_ECOLI A:[6-305] --SLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVN-GE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRV-----E---NDE---------D---A---AKAAQ----VLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
28 PsiBlast_CBE 72.9936% -24 - C1 -1RK2 RBSK_ECOLI C:[6-305] --SLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVN-GE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRV-----E---NDE---------D---A---AKAAQ----VLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
6 PsiBlast_PDB 72.4736% -24 - C1 -1RK2 RBSK_ECOLI A:[6-305] --SLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVN-GE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRV-----E---NDE---------D---A---AKAAQ----VLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
9 PsiBlast_PDB 72.2736% -30 - C1 -1GQT RBSK_ECOLI A:[6-305] --SLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVN-GE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRV-----E---NDE---------D---A---AKAAQ----VLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
29 PsiBlast_CBE 72.2336% -23 - C1 -1RK2 RBSK_ECOLI B:[6-305] --SLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVN-GE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRV-----E---NDE---------D---A---AKAAQ----VLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
27 PsiBlast_CBE 72.1336% -24 - C1 -1RK2 RBSK_ECOLI D:[6-305] --SLVVLGSINADHILNLQSFPTPGETV-TGNH-YQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVN-GE-GENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPA---R--ELPDELLALVDIITPNETEAEKL-----TGIRV-----E---NDE---------D---A---AKAAQ----VLHEKGIRTVLITLGSRGVWASVNGE--GQRVPGFRVQAVDTIAAGDTFNGALITALLE--E----K----P-LPE----AIRFAHAAAAIAVTRKGAQPSVPWREEIDAFL---
64 HHSearch 71.9438% -20 - C1 -3GO6 ? A:[19-303] APRVCVVGSVNMDLTFVVDALPRPGETV-LAAS-LTRTPGGKGANQAVAAARAGAQVQFSGAFGDDPAAAQLRAHLRANAVGLDRTVTVP-GPSGTAII-VVDAS-AENTVLVAPGANAHLTPVP-----SAVANCDVLLTQLEIPVATALAAARAAQSADAVVMVNASPAG--QDRSSLQDLAAIADVVIANEHEANDW-----PS---------------------------------PPTH----FV---------ITLGVRGARYVGADG--VFEVPAPTVTPVDTAGAGDVFAGVLAANWPR--N----PGSPAE-RLR----ALRRACAAGALATLVSGVGDCAPAAAAIDAALR--
52 Fugue 70.3137% -21 - C1 -3GO6 ? A:[19-305] APRVCVVGSVNMDLTFVVDALPRPGETV-LAAS-LTRTPGGKGANQAVAAARAGAQVQFSGAFGDDPAAAQLRAHLRANAVGLDRTVTVPG-PSGTAII-VVDAS-AENTVLVAPGANAHLTPVP-----SAVANCDVLLTQLEIPVATALAAARAAQSADAVVMVNASPAGQDR--SSLQDLAAIADVVIANEHEANDW-----PSPPT---------------------------------H----FV---------ITLGVRGARYVGADG--VFEVPAPTVTPVDTAGAGDVFAGVLAANWPR--NPGSPA----E-RLR----ALRRACAAGALATLVSGVGDCAPAAAAIDAALRAN
35 PsiBlast_CBE 68.5537% -40 - C1 -3GO6 ? B:[22-287] ---VCVVGSVNMDLTFVVDALPRPGETV-LAAS-LTRTPGGKGANQAVAAARAGAQVQFSGAFGDDPAAAQLRAHLRANAVGLDRTVTVPG-PSGTAII-VVDAS-AENTVLVAPGANAHLTPVPS-----AVANCDVLLTQLEIPVATALAAARAAQSADAVVMVNASPAGQDR--SSLQDLAAIADVVIANEHEAND------WPS--------------------------------PPTH----FV---------ITLGVRGARYVGADG--VFEVPAPTVTPVDTAGAGDVFAGVLAANWPR--N----P----G-SPAERLRALRRACAAGALATLVSG------------------
12 PsiBlast_PDB 66.3537% -33 - C1 -3GO6 ? A:[22-287] ---VCVVGSVNMDLTFVVDALPRPGETV-LAAS-LTRTPGGKGANQAVAAARAGAQVQFSGAFGDDPAAAQLRAHLRANAVGLDRTVTVPG-PSGTAII-VVDAS-AENTVLVAPGANAHLTPVPS-----AVANCDVLLTQLEIPVATALAAARAAQSADAVVMVNASPAGQDR--SSLQDLAAIADVVIANEHEAND------WPS--------------------------------PPTH----FV---------ITLGVRGARYVGADG--VFEVPAPTVTPVDTAGAGDVFAGVLAANWPR--N----P----G-SPAERLRALRRACAAGALATLVSG------------------
13 PsiBlast_PDB 65.9437% -34 - C1 -3GO7 ? A:[22-287] ---VCVVGSVNMDLTFVVDALPRPGETV-LAAS-LTRTPGGKGANQAVAAARAGAQVQFSGAFGDDPAAAQLRAHLRANAVGLDRTVTVPG-PSGTAII-VVDAS-AENTVLVAPGANAHLTPVPS-----AVANCDVLLTQLEIPVATALAAARAAQSADAVVMVNASPAGQDR--SSLQDLAAIADVVIANEHEAND------WPS--------------------------------PPTH----FV---------ITLGVRGARYVGADG--VFEVPAPTVTPVDTAGAGDVFAGVLAANWPR--N----P----G-SPAERLRALRRACAAGALATLVSG------------------