Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRGNSTEVTEFHLLGFGVQHEFQHVLFIVLLLIYVTSLIGNIGMILLIKTDSRLQTPMYFFPQHLAFVDICYTSAITP--KMLQSFTEENNLITFRGCVIQFLVYATFATSDCYLLAIMAMDCYVAICKPLRYPMIMSQTVYIQLVAGSYIIGSINASVHTGFTFSLSFCKSNKINHFFCDGLPILALSCSNIDINIILDVVFVGFDLMFTELVIIFSYIYIMVTILKMSSTAGRKKSFSTCASHLTAVTIFYGTLSYMYLQPQSNNSQENMKVASIFYGTVIPMLNPLIYSLRNKEGK 2R4S Chain:A ((38-147)) ----------------------------IVMSLIVLAIVFGNVLVITAIA-FERLQTVTNYFITSLACADLVMGLAVVPFG--------------FWTSIDVLCVTASIET-----LCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVFCLKEHKALKTLGIIMGTFTLCWLPFFILNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLK-------------------------------------

General information: TITO was launched using: RESULT: Template: 2R4S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -73884 for 540 contacts (-136.8/contact) + 2D Compatibility (PS) -8826 + (NN) 1382 + (LL) 5804 1D Compatibility (HY) -10800 + (ID) 1500 Total energy: -87824.0 ( -162.64 by residue) QMean score : 0.160

(partial model without unconserved sides chains): PDB file : Tito_2R4S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R4S-query.scw PDB file : Tito_Scwrl_2R4S.pdb: