Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLRYLFILLIILAVTSVFIGVEDLS----PLDLFDLSKQEASTLFASRLPRLISIVIAGLSMSICGLIMQQISRNKFVSPTTAGTMDWARLGILISLLLFTSASPLIKMLVAFVFA-LAGNFLFMKILERIKFNDT--IFIPLVGLMLGNIVSSIATFIAYKYDLIQNVSS---WLQGDFSLVVKGRYE----LLYLSIPLVIIAYVYADKFTLAGMGESFSVNLGLKYKRVVNIGLIIVSLITSLVILTV---GMLPFLGLIIPNIVSIYRGDNLKSSLPHTVLLGAVFVLFCDILGRIIIFPYEISIGLMVGIIGSGIFLFMLLRRKAYA 2NQ2 Chain:A ((13-330)) -----LTLLLVITAVISLGIGRYSLSVPQI-------DPVQQQVIFQVRLPRILTALCVGAGLALSGVVLQGIFRNPLVNPHIIGVTSGSAFGGTLAIFFGFSLYGLFTSTILFGFGTLALVFLF-----SFK----SLLMLILIGMILSGLFSALVSLLQYISDTEEKLPSIVFWLMGSFA---TSNWEKLLFFFVPFLLCSSILLSLSWRLNLLSLDEKEAKALGVK---MAPLRWLVIFLSGSLVACQVAISGSIGWVGLIIPHLSRMLVGANHQSLLPCTMLVGATYMLLVDNVAR-SLSDAEIPISILTALIGAPLFGVLVYKL----

General information: TITO was launched using: RESULT: Template: 2NQ2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1294 -258997 -200.15 -911.96 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -200.15 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_2NQ2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NQ2-query.scw PDB file : Tito_Scwrl_2NQ2.pdb: