Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRISIEEVKHVAHLARLAITEEEAKMFTEQLDSIISFAEELNEVNTDNVEPTTHVLKMKNVMREDEAGKGLPVEDVMKNAPDHKDGYIRVPSILD 2DF4 Chain:C ((2-96)) -TKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDSADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMN

General information: TITO was launched using: RESULT: Template: 2DF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 110 -11313 -102.84 -119.08 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.86 3D Compatibility (PKB) : -102.84 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_2DF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DF4-query.scw PDB file : Tito_Scwrl_2DF4.pdb: