Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEAEVLKYEAFTSSPGKGNPAGVVLQGDDYTEDEMQIIAERAGYSETSFIRKSESADLELRYFTPGHEMNLCGHATVASLYALCEKGMLESGKTYSIQTKAGILPVKIS-EKDGRIHITLEQASPQFKPFTGDRKKLADALGITDEDFHEDLPIVFGSTGIWTAIVPLKSLEASKKMVPDNKQFPEVLVDLPKASVHPFTFETVHPDSDLHGRHFSSPYSGTIEDPVTGTASGVMGAYMKHYGNAE---QHKFIIEQGQEIGKDGKVEIEMNE--AGGHVKVNMTGTAVYSETRILKI 3EDN Chain:A ((2-291)) -KTINVFHYDAFTNKPNMGNPAGIVLDADGLTEEEMQRIAEKVGFNETSFVLSSEVADIRMRYFTPGYEMDLCGHGTVGTIYALRERGLLEEKASLTIETKAGILPIQIGVNENGETFIKMRQTAPQFKDFAGSKEELAHSIGLEVNDLDVSLPIVYGSTGNWTVIVPVKNLDVCERMKPNNEVFPSVLKEIPNASIHPICLETYDEKVHMHGRHFSSAYAGTIEDPVTGTASGVMGAYYATYVEKDFDHEMELIVEQGQEIHKDGRVTVYVTKDVESEKLQIDIAGTAVY--------

General information: TITO was launched using: RESULT: Template: 3EDN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1725 -127014 -73.63 -447.23 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -73.63 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.557

(partial model without unconserved sides chains): PDB file : Tito_3EDN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EDN-query.scw PDB file : Tito_Scwrl_3EDN.pdb: