Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIEIGQKAPDLELKGDHGETVKLSDYKGKYIVLYFYPKDMTPGCTTEACDFRDSHESFAELDAVIIGVSPDSQEKHGKFKEKHNLPFLLLVDDEHKLAEAFDVWKLKKNFGKEYMGIERSTFLIDKEGRLIKEWRKVKVKDHVAEALQTLKDMSEK 5ENU Chain:A ((1-153)) MSVEVDRQVPDFTAPATGGD-ISLSDLKGRKLVLYFYPKDNTPGCTTEGLQFRELYPKFKKAGAEIIGVSRDSLRSHDNFKAKLELPFPLISDADEALCALFDVIKMKKMYGKEVRGIERSTFLIDADGVLRQAWRGIKVPGHVDDVLSAVQAL---

General information: TITO was launched using: RESULT: Template: 5ENU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 739 -86170 -116.60 -563.20 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -116.60 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_5ENU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ENU-query.scw PDB file : Tito_Scwrl_5ENU.pdb: