Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIPVKKNLVSEAKYALKCPNAMSAEYITIHNTANDASAANEISYMIGNTSSTSFHFAVDDQEVIQGLPLNRNAWHTGDGTNGPGNRKSIGVEICYSKSGGPKYEAAEALAISFVAQLLKERGWGIDRVRKHQDWSGKYCPHRILSEGRWDQVKAAIEKELNGGVSAKKAAVSSSASEYHVKKGDTLSGIAASHGASVKTLQSINHITDPNHIKIGQVIKLPQTASASKSHAASSYPLPSGVIKVTSPLTQGTKVKQVQTALAALYFYPDKGAKNHGVDGVYGPKTANAVKRFQSVSGLTADGIYGPKTKAKMEEKL 2L47 Chain:A ((1-160)) --MEIQKKLVDPSKYGTKCPYTMKPKYITVHNTYNDAPAENEVSYMISNNNEVSFHIAVDDKKAIQGIPLERNAWACGDG-NGSGNRQSISVEICYSKSGGDRYYKAEDNAVDVVRQLMSMYNIPIENVRTHQSWSGKYCPHRMLAEGRWG---AFIQKVKNGNVA-------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2L47.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 954 -52035 -54.54 -325.22 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -54.54 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_2L47.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L47-query.scw PDB file : Tito_Scwrl_2L47.pdb: