Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFPKKAEIIAVGSELLLGQIANTNAQFISKQLAEIGVNVFYHTAVGDNPERLKQVIRIAEERSDFIIFSGGLGPTKDDLTKETIANTLGRPLVLNDEAFQSIEDYFKRTKRTMSPNNRKQALVIEGSDVLANHFGMAPGMLTEHESRYYMLLPGPPSELRPMFENEAKPLLLKKMGSNEKIVSTVLRFFGIGESQLEADL------EDIIDAQTNPTIAPLAADGEVTLR----LTAKHADEKETERLLKETEAVILERVGEFFYGYDDTSLVKELSIACKEKGITISAAESFTGGLFSEWLTDHSGASKLFAGGVVCYTNDVKQNVLGVKKETLDRFGAVSKECASELAKGVQKLTGSDIGISFTGVAGPDAQEGHEPGHVFIGISANGKEEVHEFHFAGSRTGIRKRGAKYGCHLILKLLEQK 4CT8 Chain:A ((2-393)) ----ERAEILGVGTELLYGETLDTNTAEIARSLKPYALKVERTLRVADEVAPLAREVEEAFARARLVVLSGGLGPTPDDVTREAVALALGEPLELDEAVLGEIEAFFRARGRAMPEANRKQAMRIPSATWLKNPRGTAPGWWVRKGGKDLVLLPGPPPEWRPMWQEVLPRLGLPRRPYAERVLKT----WGIGESEIVERLGPLFVREEEVEVGTYPKVHGV----EVVVRGREDRVAELA-ERIKKKLLKE------------VWGEGEMTLAEAVKRRMEREGATLSTMESLTGGLLGAEITRVPGASRFYLGGVVSYSVGAKAR-FGVPQDLLSR--TVSAETARAMAEAARSLFGSTYALATTGVAGPDPLEGEPPGTVYVALAGPTGAEVRRYRFPGDRETVRLRSVYAALALLV------

General information: TITO was launched using: RESULT: Template: 4CT8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2015 -168336 -83.54 -440.67 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -83.54 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_4CT8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CT8-query.scw PDB file : Tito_Scwrl_4CT8.pdb: