Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNSEHFNLIQRALDATANELKELGTDSSPSVISHAQ---TDLEKAVE----HIYSTDHPFLSSHVINRK 1NI4 Chain:A ((305-350)) ---------------ASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPF---------

General information: TITO was launched using: RESULT: Template: 1NI4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 8 481 60.06 12.32 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 60.06 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_1NI4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NI4-query.scw PDB file : Tito_Scwrl_1NI4.pdb: