Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYIKAGKWLTVFLTFLGILLFIDLFPKEEHDQKTKSKQKPDYRAAYHFTTPDKWKNDPQKPIYFDGKYHYFYLYNRDYPKGNGTEWRHAVSEDLVHWTDEGVAIPKYTNPDGDIWTGSVVVDKENTAGFGKNALVAIVTQPSAKDKK-QEQYLWYSTDKGKSFKFYSGNPVMPNPGTDD--------------FRDPKVIWDDQDNKWVMVMAEGSKIGFYESDNLKDWHYTSGF-FPEQA-GMVECPDLYMMRASDGTNKWVLGASANGKPWGKPNTYAYWTGSFDGKEFKADQTEAQWLDYGFDWYGGVTFEDSKSTDPLEKRYALAWMNNWDY-ANNTPT-MKNGFNGTDSVIRELRLKEQDGT-YSLVSQPIEALEQLTVSTDEIEDQDVNGSKTLSITGDTYQLDTDLSWSELKNAGVRLRESEDQKRHIDVGIFAEGGYAYVNRAATNQPDKS-NTYVESKAPYDVNKRKVHLKILVDKTTIEVFVGDGKTVFSNEVFPKPEDKGITLYSDGGTASFKNITVKHFDSIHE 4FFF Chain:A ((41-517)) --------------------------------------------AVYHMTPPSGWLCDPQRPVTTHGAYQLYYLHS-DQNNGPG-GWDHASTTDGVAFTHHGTVMP--LRPDFPVWSGSAVVDTANTAGFGAGAVVALATQPTDGVRKYQEQYLYWSTDGGFTFTALP-DPVIVN--TDGRAATTPAEIENAEWFRDPKIHWDTARGEWVCVIGRLRYAAFYTSPNLRDWTLRRNFDYPNHALGGIECPDLFEITADDGTRHWVLAASMDAYGIGLPMTYAYWTGTWDGEQFHADDLTPQWLDWGWDWYAAVTWPSIDA--PETKRLAIAWMNNWKYAARDVPTDASDGYNGQNSIVRELRLARQPGGWYTLLSTPVAALTNYVTATTTLPDRTVDGSAVLPWNGRAYEIELDIAWDTATNVGISVGRSPDGTRHTNIGKY--GADLYVDRGPSDLAGYSLAPYSRAAAPIDPGARSVHLRILVDTQSVEVFVNAGHTVLSQQVHFAEGDTGISLYTDGGPAHFTGIVVREI-----

General information: TITO was launched using: RESULT: Template: 4FFF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2713 56885 20.97 124.75 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 20.97 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_4FFF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FFF-query.scw PDB file : Tito_Scwrl_4FFF.pdb: