Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVRFLQISDLHFQFQNYDTIVMRDKLLDFIEVLRRESDFDFLLLTGDIAHKGD-VYNEDVKEYLNGIIKSMGLSKNNVHLVPGNHDISRDMTRTLLIDSIMKSPNPSEMLDKLDQKATNILVEGQRKFFDFYEDFM---GVKYPEEDLHFLY-QSEKYNVLSINTC-----LLSDKKGEEGTLLTAQMKLYKAIRKMNKEKNKGKVLNIAIGHHTL-------GCIESSERESIKAHFDDYFIDLYLAGHVHDSSFNITANTNENPFLELVSGAIIKDEYATPEFISVDVNLDNGETEVTYYIWNTEYKYWSKNNQGGRRLQEGKLNYKINRLESLIEKEIEDNDDKIDEDEFKSFIIDFHEYRESYKTFTSNFDNQIGLDKKFYDMKSGETFKRKFDSYSEYFGVINHIMDSTSYVSADKKELIAETIVDKYLEFHNQYNNGDEIFVKIVNEIYLECHSVLPYSKALTKKYIKILTCWCIYECEIFNDNKRSVKND 2XMO Chain:A ((94-306)) ----------------------------------------DVLIISGDLTNNGEKTSHEELAKKLTQVEKN----GTQVFVVPGNHDINNPWARKFEKDKQL----PTDTISPTD-------------FSKIYSDFGYEDAISSDEFSLSYLAAPSSKVWLLMLDTAIYKTNMQQGNPTTEGGLTAGTLDWIKESSALAK-KNGAKL--IPVLHHNLTDHNDVKGYTINYNQQVIDA-LTEGAMDFSLSGHIHTQNIRSAKSTDGKEITDIVTNAL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2XMO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 989 45193 45.70 232.95 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 45.70 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_2XMO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XMO-query.scw PDB file : Tito_Scwrl_2XMO.pdb: