Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGELANYKRLEKVGEGTYGVVYKALDLRPGQGQRVVALKKIRLESEDEGVPSTAIREISLLKELKDDNIVRLYDIVHSDAHKLYLVFEFLDLDLKRYME-----GIPKDQPLGADIVKKFMMQLCKGIAYCHSHRILHRDLKPQNLLINKDGNLKLGDFGLARAFGVPLRAYTHEIVTLWYRAPEVLLGGKQYSTGVDTWSIGCIFAEMCNRKPIFSGDSEIDQIFKIFRVLGTPNEAIWPDIVYLPDFKPSFPQWRRKDLSQVVPSLDPRGIDLLDKLLAYDPINRISARRAAIHPYFQES 3EZR Chain:A ((3-290)) ----MENFQKVEKIGEGTYGVVYKARNKLTGE---VVALKKIR-------VPSTAIREISLLKELNHPNIVKLLDVIHTE-NKLYLVFEFLHQDLKKFMDASALTGIP------LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADF------------------TLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQD-

General information: TITO was launched using: RESULT: Template: 3EZR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -169600 for 2000 contacts (-84.8/contact) + 2D Compatibility (PS) -27164 + (NN) -9623 + (LL) 1972 1D Compatibility (HY) -34800 + (ID) 8300 Total energy: -247515.0 ( -123.76 by residue) QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_3EZR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EZR-query.scw PDB file : Tito_Scwrl_3EZR.pdb: