Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLGIMGTNWITDSFIEGAINSGEWNLTAVYSRTEEKARAFGEKYGELTYFTDIEEMGKSDALDAVYIASPNALHYQHAVSLLKNKKHVIVEKPIFSTVAELEHAHQIARENNVFLFEAARHIQEPNFKRLQENIEKVGTIHGATLAYMKYSSRYDQVLNGEEPNIFSLKFSGGSIVDLGVYPLYSAITLFGEPVKATYFATKLPTGVDGLGPIILEYPTFNITIIQGKNSQSFLPSEIYGQKGTLIVDPLTGIEKITFYDNATKEETELAGPVVANDMQFEAAEFARIIEQSDRDTYE-YLADLSLKVLRVSNELRHQNDIWFDAEK 4NHE Chain:A ((5-328)) LKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFASRYQNIQLFDQLEVFFKS-SFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVSQPQEWFDLIQTAEKNNCFIFEAARNYHEKAFTTIK-NFLADKQVLGADFNYAKYSSKMPDLLAGQTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYPDYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDH-QGNQVQLPIQQAPHTMTEEVAAFAHMIQQPDLNLYQTWLYDAG-SVHELLYTMRQTAGIRFEAEK

General information: TITO was launched using: RESULT: Template: 4NHE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -170375 for 2785 contacts (-61.2/contact) + 2D Compatibility (PS) -34994 + (NN) -15664 + (LL) 348 1D Compatibility (HY) -25200 + (ID) 6650 Total energy: -252535.0 ( -90.68 by residue) QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_4NHE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NHE-query.scw PDB file : Tito_Scwrl_4NHE.pdb: