Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLVPRFRKQWFAYLTVLCLALAAAVSFGVPAKAAENPQTSVSNTGKEADATKNQTSKADQVSAPYEGTGKTSKSLYGGQTELEKNIQTLQPSSIIGTDERTRISSTTSFPYRATVQLSIKYPNTSSTYG---CTGFLVNPNTVVTAGHCVY-SQDHGWASTITAAPGRNGSSYPYGTYSGTMFYSVKGWTESKDTNYDYGAIKLNGSPGNTVGWYGYRTTNSSSPVGLSSSVTGFPCDKTFGT----MWSDTKPIRSAETYKLTYTTDTYGCQSGSPVYRNYSDTGQTAIAIHTNGGSSYNLGTRVTNDVFNNIQYWANQ 1P3C Chain:A ((2-189)) ----------------------------------------------------------------------------------------------VIGDDGRTKVANTRVAPYN-----SIAYI----TFGGSSCTGTLIAPNKILTNGHCVYNTASRSYSAKGSVYPGMNDSTAVNGSANMTEFYVPSGYINTGASQYDFAVIKTDTNIGNTVGYRSIRQV--TNLTGTTIKISGYPGDKMRSTGKVSQWEMSGSVTREDTNLAYYTIDTFSGNSGSAML----DQNQQIVGVHNAG------------------------

General information: TITO was launched using: RESULT: Template: 1P3C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 996 -55737 -55.96 -309.65 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -55.96 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.217

(partial model without unconserved sides chains): PDB file : Tito_1P3C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P3C-query.scw PDB file : Tito_Scwrl_1P3C.pdb: