Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYIPCMITIGNFICGLLAIHSLLYHNIHSAVLFIFTGMFLDFFDGMAARKLNAVSDMGR------ELDSF-ADLVTF-GVAPSMLAYSVALYTLPFIGILCALT-YSICGMLRLSKFNIEQSKLPTFIGMPIPFAGMCLVILSFTYNPILLAIGTCGLSYLMVSKIKFPHFKKHAAENLESGRWN 4H7N Chain:A ((75-170)) ------------------------------------------------------VNDTGRLSITVLEIDSFLASIDRWCGLAPELLQTSAKNTSIPFIALQQSLVPYPLVGVISPWNFPLTLSMIDT---IPALLAGCAVVVKPSEIAPRFVA---------------------------------

General information: TITO was launched using: RESULT: Template: 4H7N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 262 -28133 -107.38 -323.37 target 2D structure prediction score : 0.15 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -107.38 2D Compatibility (Sec. Struct. Predict.) : 0.15 1D Compatibility (Hydrophobicity) : 0.57 QMean score : -0.207

(partial model without unconserved sides chains): PDB file : Tito_4H7N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H7N-query.scw PDB file : Tito_Scwrl_4H7N.pdb: