Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKQQPISQRKLLGVAGLGWLFDAMDVGILSFIIAALHVEW----NLSPEEMKWIGSVNSIGMAAGAFLFGLLADRIGRKKVFIITLLCFSIGSGISAFVTS-------LSAFLILRFVIGMGLGGELPVASTLVSEAVVPEKRGRVIVLLESFWAVGWLAAALISYFVIPSF--------------GWQAALLLTALTAFYALYLRTSLPDSPKYESLSAKKRSMWENVKSVWARQYIRPTVMLSIV-----------------------WFCVVFSYYGMFLWLPSVMLLKGFSMIQSFEYVLLMTLAQ----------LPGYFSAAWLIEKAGRKWILVV-YLIGTAGSAYFFGTADSLS---LLLTAGVLLSFFNLGAWGVLYAYTPEQYPTAIRATGSGTTAAFGRIGGIFGPLLVGTLAARHISFSVIFSIFCIAILLAVACILI---MGKETKQTELE 4J05 Chain:A ((33-515)) --------QIKLVLLAGVGFFLDAYDLFIINQVAPMLAQVYFPKTGLPAQRQDLMKAAANIGCVVGQVMFGVLGDSFGRKFVYGKELILIIVATIFQMSAPSHWDGNRVLTWITICRVFLGIGIGGDYPMSATVVSDRANIHRRGTLLCFIFANQGWGSFVGSLVTIVTISGFKHRLKSGHTHDVDKAWRILIGLSLIPAFGTLYQR-----------------------------------------WQEFVAYFSTWNHFRNLLGSMLGWFLVDIAFYGINLNQSVVLAQIGFAGKTGDVYDKLFQLATGNIIVTALGFLPGYYFTLFLIDIVGRKKLQFMGFIMSGLFLAILAGEIDHIGKGPLLACFTFMQFFFNFGANTTTFIVAAELFPTRIRASAHGISAAAGKCGAILSSLVFNQLKAKIGTSAVLWIFFSTCILGFISTFLIDETMGVDPDEKDLE

General information: TITO was launched using: RESULT: Template: 4J05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1803 -281380 -156.06 -801.65 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -156.06 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_4J05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J05-query.scw PDB file : Tito_Scwrl_4J05.pdb: