Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWEQLYDPFGNEYVSALVALTPILFFLLALTVLKMKGILAAFLTLAVSFFVSVWAFHMPVEKAISSVLLGIGSGLWPIGYIVLMAVWLYKIAVKTGKFTIIRSSIAGISPDQRLQLLLIGFCFNAFLEGAAGFGVPIAISAALLVELGFKPLKAAALCLIANAASGAFGAIGIPVITGAQIGDLSALELSRTLMWTLPMISFLIPFLLVFLLDRMKGIKQTWPALLVVSGGYTAVQTLTMAVLGPELANILAALFSMGGLALFLRKWQPKEIYREEGAGDAGEKKAYRAADIAKAWSPFYILTAAITIWSLPAFKALFQEGGLLYQSTLLFKMPFLHQQIMKMPPIAPSAMPLDAVFKVDLLSATGTAILAAVIVTGLFSKKFSSRDAFASLKETGKELWVPIMTICFVMGFANLANFAGLSSSIGLALAKTGDLFPFVSPVLGWIGVFITGSVVSNNALFGHLQVVTGAQIGAGSDLLL---AANTAGGVMAKLV-SPQSIAIAAAAVGQTGKESKLFKRTVAYSLILLLIICIWTFILARLGV 3GVD Chain:A ((192-252)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGDRHPKIRE-----GVMIGAGA----KILGNIEVGRGAKIGAGSVVLQSVPAHTTAAGVPARIVGKPES--------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GVD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 235 -27824 -118.40 -488.13 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.41 3D Compatibility (PKB) : -118.40 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.41 QMean score : 0.266

(partial model without unconserved sides chains): PDB file : Tito_3GVD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GVD-query.scw PDB file : Tito_Scwrl_3GVD.pdb: