Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKSWKPQELSISYHQFTVFQKDSTPPVMDWTDEAIEKGYAAADGAISFEAQRNTKAFILFRLNSSETVNSYEKKVTVPFHVTENGIHIESIMSKRLSFDLPKGDYQLTCWTVPAEMSDLHADTYIIDAVSV 4MQD Chain:A ((2-132)) -IKSWKPQELSISYHQFTVFQKDSTPPVMDWTDEAIEKGYAAADGAISFEAQRNTKAFILFRLNSSETVNSYEKKVTVPFHVTENGIHIESIMSKRLSFDLPKGDYQLTCWTVPAEMSDLHADTYIIDAVSV

General information: TITO was launched using: RESULT: Template: 4MQD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 674 -77701 -115.28 -593.14 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -115.28 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.744

(partial model without unconserved sides chains): PDB file : Tito_4MQD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MQD-query.scw PDB file : Tito_Scwrl_4MQD.pdb: